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| - | [[Image:232d.gif|left|200px]] | |
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| - | {{Structure
| + | ==THE HIGH RESOLUTION CRYSTAL STRUCTURE OF THE DNA DECAMER D(AGGCATGCCT)== |
| - | |PDB= 232d |SIZE=350|CAPTION= <scene name='initialview01'>232d</scene>, resolution 1.300Å
| + | <StructureSection load='232d' size='340' side='right'caption='[[232d]], [[Resolution|resolution]] 1.30Å' scene=''> |
| - | |SITE=
| + | == Structural highlights == |
| - | |LIGAND= <scene name='pdbligand=NCO:COBALT HEXAMMINE ION'>NCO</scene>
| + | <table><tr><td colspan='2'>[[232d]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=232D OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=232D FirstGlance]. <br> |
| - | |ACTIVITY=
| + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.3Å</td></tr> |
| - | |GENE=
| + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=NCO:COBALT+HEXAMMINE(III)'>NCO</scene></td></tr> |
| - | }}
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=232d FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=232d OCA], [https://pdbe.org/232d PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=232d RCSB], [https://www.ebi.ac.uk/pdbsum/232d PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=232d ProSAT]</span></td></tr> |
| - | | + | </table> |
| - | '''THE HIGH RESOLUTION CRYSTAL STRUCTURE OF THE DNA DECAMER D(AGGCATGCCT)'''
| + | __TOC__ |
| - | | + | </StructureSection> |
| - | | + | [[Category: Large Structures]] |
| - | ==Overview== | + | [[Category: Neidle S]] |
| - | The crystal structure of the DNA decamer d(AGGCATGCCT) has been determined to a resolution of 1.3 A and R factor of 13.9%. The structure has a unique conformation with each of the decamer single strands forming base-pairing interactions with two symmetry-related strands. The central eight bases of the decamer form an A-DNA octamer duplex with one symmetry-related strand whilst the terminal 5'-A and T-3' bases are flipped out and away from the octamer helix axis to form base-pairing interactions with a second symmetry-related strand. These A.T base-pairs lie perpendicular to the crystallographic c axis and pack within the unit cell in conjunction with a symmetry-related A.T base-pair displaced by 3.4 A degrees along the c axis. A novel base triplet interaction of the type A*(G.C) is present in the structure with interaction from the major groove side of the terminal 5'-A base to the minor groove of the central A-DNA octamer. This structure reports the first example of cobalt hexammine binding to a right-handed DNA duplex. The crystallographic asymmetric unit contains two cobalt hexammine ligands with one site in the major groove coordinating via hydrogen bonds to the 5'-AGG bases, and the second site located between DNA molecules and interacting with the oxygen atoms of phosphate groups.
| + | [[Category: Nunn CM]] |
| - | | + | |
| - | ==About this Structure== | + | |
| - | 232D is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/ ]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=232D OCA].
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| - | | + | |
| - | ==Reference== | + | |
| - | The high resolution crystal structure of the DNA decamer d(AGGCATGCCT)., Nunn CM, Neidle S, J Mol Biol. 1996 Feb 23;256(2):340-51. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/8594201 8594201]
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| - | [[Category: Protein complex]] | + | |
| - | [[Category: Neidle, S.]] | + | |
| - | [[Category: Nunn, C M.]]
| + | |
| - | [[Category: NCO]]
| + | |
| - | [[Category: a-dna]] | + | |
| - | [[Category: double helix]] | + | |
| - | [[Category: flipped-out base]] | + | |
| - | | + | |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 15:42:37 2008''
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