4qx0

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==Cry3A Toxin structure obtained by Serial Femtosecond Crystallography from in vivo grown crystals isolated from Bacillus thuringiensis and data processed with the cctbx.xfel software suite==
==Cry3A Toxin structure obtained by Serial Femtosecond Crystallography from in vivo grown crystals isolated from Bacillus thuringiensis and data processed with the cctbx.xfel software suite==
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<StructureSection load='4qx0' size='340' side='right' caption='[[4qx0]], [[Resolution|resolution]] 2.80&Aring;' scene=''>
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<StructureSection load='4qx0' size='340' side='right'caption='[[4qx0]], [[Resolution|resolution]] 2.80&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
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<table><tr><td colspan='2'>[[4qx0]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4QX0 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4QX0 FirstGlance]. <br>
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<table><tr><td colspan='2'>[[4qx0]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Bacillus_thuringiensis_serovar_tenebrionis Bacillus thuringiensis serovar tenebrionis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4QX0 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4QX0 FirstGlance]. <br>
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</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[4qx1|4qx1]], [[4qx2|4qx2]], [[4qx3|4qx3]]</td></tr>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.8&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4qx0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4qx0 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4qx0 RCSB], [http://www.ebi.ac.uk/pdbsum/4qx0 PDBsum]</span></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4qx0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4qx0 OCA], [https://pdbe.org/4qx0 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4qx0 RCSB], [https://www.ebi.ac.uk/pdbsum/4qx0 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4qx0 ProSAT]</span></td></tr>
</table>
</table>
== Function ==
== Function ==
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[[http://www.uniprot.org/uniprot/CR3AA_BACTT CR3AA_BACTT]] Promotes colloidosmotic lysis by binding to the midgut epithelial cells of Coleoptera.
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[https://www.uniprot.org/uniprot/CR3AA_BACTT CR3AA_BACTT] Promotes colloidosmotic lysis by binding to the midgut epithelial cells of Coleoptera.
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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It has long been known that toxins produced by Bacillus thuringiensis (Bt) are stored in the bacterial cells in crystalline form. Here we describe the structure determination of the Cry3A toxin found naturally crystallized within Bt cells. When whole Bt cells were streamed into an X-ray free-electron laser beam we found that scattering from other cell components did not obscure diffraction from the crystals. The resolution limits of the best diffraction images collected from cells were the same as from isolated crystals. The integrity of the cells at the moment of diffraction is unclear; however, given the short time ( approximately 5 micros) between exiting the injector to intersecting with the X-ray beam, our result is a 2.9-A-resolution structure of a crystalline protein as it exists in a living cell. The study suggests that authentic in vivo diffraction studies can produce atomic-level structural information.
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Protein crystal structure obtained at 2.9 A resolution from injecting bacterial cells into an X-ray free-electron laser beam.,Sawaya MR, Cascio D, Gingery M, Rodriguez J, Goldschmidt L, Colletier JP, Messerschmidt MM, Boutet S, Koglin JE, Williams GJ, Brewster AS, Nass K, Hattne J, Botha S, Doak RB, Shoeman RL, DePonte DP, Park HW, Federici BA, Sauter NK, Schlichting I, Eisenberg DS Proc Natl Acad Sci U S A. 2014 Aug 18. pii: 201413456. PMID:25136092<ref>PMID:25136092</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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==See Also==
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</div>
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*[[Delta-endotoxin|Delta-endotoxin]]
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== References ==
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*[[Pesticidal crystal protein|Pesticidal crystal protein]]
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<references/>
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__TOC__
__TOC__
</StructureSection>
</StructureSection>
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[[Category: Botha, S]]
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[[Category: Bacillus thuringiensis serovar tenebrionis]]
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[[Category: Boutet, S]]
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[[Category: Large Structures]]
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[[Category: Brewster, A S]]
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[[Category: Botha S]]
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[[Category: Cascio, D]]
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[[Category: Boutet S]]
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[[Category: Colletier, J P]]
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[[Category: Brewster AS]]
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[[Category: DePonte, D P]]
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[[Category: Cascio D]]
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[[Category: Doak, R B]]
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[[Category: Colletier J-P]]
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[[Category: Eisenberg, D]]
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[[Category: DePonte DP]]
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[[Category: Federici, B A]]
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[[Category: Doak RB]]
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[[Category: Gingery, M]]
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[[Category: Eisenberg D]]
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[[Category: Goldschmidt, L]]
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[[Category: Federici BA]]
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[[Category: Hattne, J]]
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[[Category: Gingery M]]
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[[Category: Koglin, J E]]
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[[Category: Goldschmidt L]]
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[[Category: Messerschmidt, M]]
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[[Category: Hattne J]]
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[[Category: Nass, K]]
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[[Category: Koglin JE]]
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[[Category: Park, H W]]
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[[Category: Messerschmidt M]]
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[[Category: Rodriguez, J]]
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[[Category: Nass K]]
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[[Category: Sauter, N K]]
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[[Category: Park H-W]]
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[[Category: Sawaya, M R]]
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[[Category: Rodriguez J]]
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[[Category: Schlichting, I]]
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[[Category: Sauter NK]]
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[[Category: Shoeman, R L]]
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[[Category: Sawaya MR]]
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[[Category: Williams, G J]]
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[[Category: Schlichting I]]
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[[Category: In vivo crystal]]
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[[Category: Shoeman RL]]
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[[Category: Insecticidal toxin]]
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[[Category: Williams GJ]]
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[[Category: Lcl]]
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[[Category: Microcrystal]]
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[[Category: Serial femtosecond crystallography]]
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[[Category: Sfx]]
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[[Category: Toxin]]
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[[Category: X-ray free-electron laser]]
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Current revision

Cry3A Toxin structure obtained by Serial Femtosecond Crystallography from in vivo grown crystals isolated from Bacillus thuringiensis and data processed with the cctbx.xfel software suite

PDB ID 4qx0

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