This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.

Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.


4f7z

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
Current revision (15:18, 14 March 2024) (edit) (undo)
 
(2 intermediate revisions not shown.)
Line 1: Line 1:
 +
==Conformational dynamics of exchange protein directly activated by cAMP==
==Conformational dynamics of exchange protein directly activated by cAMP==
-
<StructureSection load='4f7z' size='340' side='right' caption='[[4f7z]], [[Resolution|resolution]] 2.60&Aring;' scene=''>
+
<StructureSection load='4f7z' size='340' side='right'caption='[[4f7z]], [[Resolution|resolution]] 2.60&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
-
<table><tr><td colspan='2'>[[4f7z]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4F7Z OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4F7Z FirstGlance]. <br>
+
<table><tr><td colspan='2'>[[4f7z]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4F7Z OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4F7Z FirstGlance]. <br>
-
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr>
+
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.6&#8491;</td></tr>
-
<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">Rapgef4, Cgef2, Epac2 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10090 Mus musculus])</td></tr>
+
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr>
-
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4f7z FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4f7z OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4f7z RCSB], [http://www.ebi.ac.uk/pdbsum/4f7z PDBsum]</span></td></tr>
+
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4f7z FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4f7z OCA], [https://pdbe.org/4f7z PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4f7z RCSB], [https://www.ebi.ac.uk/pdbsum/4f7z PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4f7z ProSAT]</span></td></tr>
</table>
</table>
== Function ==
== Function ==
-
[[http://www.uniprot.org/uniprot/RPGF4_MOUSE RPGF4_MOUSE]] Guanine nucleotide exchange factor (GEF) for RAP1A, RAP1B and RAP2A small GTPases that is activated by binding cAMP. Seems not to activate RAB3A. Involved in cAMP-dependent, PKA-independent exocytosis through interaction with RIMS2.<ref>PMID:11056535</ref>
+
[https://www.uniprot.org/uniprot/RPGF4_MOUSE RPGF4_MOUSE] Guanine nucleotide exchange factor (GEF) for RAP1A, RAP1B and RAP2A small GTPases that is activated by binding cAMP. Seems not to activate RAB3A. Involved in cAMP-dependent, PKA-independent exocytosis through interaction with RIMS2.<ref>PMID:11056535</ref>
-
<div style="background-color:#fffaf0;">
+
-
== Publication Abstract from PubMed ==
+
-
Exchange proteins directly activated by cAMP (EPACs) are important allosteric regulators of cAMP-mediated signal transduction pathways. To understand the molecular mechanism of EPAC activation, we have combined site-directed mutagenesis, X-ray crystallography, and peptide amide hydrogen/deuterium exchange mass spectrometry (DXMS) to probe the structural and conformational dynamics of EPAC2-F435G, a constitutively active EPAC2 mutant. Our study demonstrates that conformational dynamics plays a critical role in cAMP-induced EPAC activation. A glycine mutation at 435 position shifts the equilibrium of conformational dynamics towards the extended active conformation.
+
-
 
+
-
Structural analyses of a constitutively active mutant of exchange protein directly activated by cAMP.,White MA, Li S, Tsalkova T, Mei FC, Liu T, Woods VL Jr, Cheng X PLoS One. 2012;7(11):e49932. doi: 10.1371/journal.pone.0049932. Epub 2012 Nov 26. PMID:23189173<ref>PMID:23189173</ref>
+
-
 
+
-
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
+
-
</div>
+
== References ==
== References ==
<references/>
<references/>
__TOC__
__TOC__
</StructureSection>
</StructureSection>
 +
[[Category: Large Structures]]
[[Category: Mus musculus]]
[[Category: Mus musculus]]
-
[[Category: Cheng, X]]
+
[[Category: Cheng X]]
-
[[Category: Liu, T]]
+
[[Category: Liu T]]
-
[[Category: Mei, F C]]
+
[[Category: Mei FC]]
-
[[Category: Tsalkova, T N]]
+
[[Category: Tsalkova TN]]
-
[[Category: White, M A]]
+
[[Category: White MA]]
-
[[Category: Woods, V L]]
+
[[Category: Woods VL]]
-
[[Category: Auto-inhibition]]
+
-
[[Category: Cdc25 homology domain]]
+
-
[[Category: Cyclic nucleotide]]
+
-
[[Category: Exchange factor]]
+
-
[[Category: Exocytosis]]
+
-
[[Category: Gef]]
+
-
[[Category: Regulation]]
+

Current revision

Conformational dynamics of exchange protein directly activated by cAMP

PDB ID 4f7z

Drag the structure with the mouse to rotate

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/4f7z"
Personal tools