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299d

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[[Image:299d.jpg|left|200px]]
 
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{{Structure
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==CAPTURING THE STRUCTURE OF A CATALYTIC RNA INTERMEDIATE: THE HAMMERHEAD RIBOZYME==
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|PDB= 299d |SIZE=350|CAPTION= <scene name='initialview01'>299d</scene>, resolution 3.000&Aring;
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<StructureSection load='299d' size='340' side='right'caption='[[299d]], [[Resolution|resolution]] 3.00&Aring;' scene=''>
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|SITE=
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== Structural highlights ==
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|LIGAND=
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<table><tr><td colspan='2'>[[299d]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=299D OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=299D FirstGlance]. <br>
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|ACTIVITY=
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3&#8491;</td></tr>
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|GENE=
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=299d FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=299d OCA], [https://pdbe.org/299d PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=299d RCSB], [https://www.ebi.ac.uk/pdbsum/299d PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=299d ProSAT]</span></td></tr>
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}}
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</table>
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'''CAPTURING THE STRUCTURE OF A CATALYTIC RNA INTERMEDIATE: THE HAMMERHEAD RIBOZYME'''
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==See Also==
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*[[Ribozyme 3D structures|Ribozyme 3D structures]]
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__TOC__
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==Overview==
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</StructureSection>
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The crystal structure of an unmodified hammerhead RNA in the absence of divalent metal ions has been solved, and it was shown that this ribozyme can cleave itself in the crystal when divalent metal ions are added. This biologically active RNA fold is the same as that found previously for two modified hammerhead ribozymes. Addition of divalent cations at low pH makes it possible to capture the uncleaved RNA in metal-bound form. A conformational intermediate, having an additional Mg(II) bound to the cleavage-site phosphate, was captured by freeze-trapping the RNA at an active pH prior to cleavage. The most significant conformational changes were limited to the active site of the ribozyme, and the changed conformation requires only small additional movements to reach a proposed transition-state.
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[[Category: Large Structures]]
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[[Category: Arnold JRP]]
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==About this Structure==
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[[Category: Klug A]]
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299D is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/ ]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=299D OCA].
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[[Category: Murray JB]]
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[[Category: Scott WG]]
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==Reference==
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[[Category: Stoddard BL]]
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Capturing the structure of a catalytic RNA intermediate: the hammerhead ribozyme., Scott WG, Murray JB, Arnold JR, Stoddard BL, Klug A, Science. 1996 Dec 20;274(5295):2065-9. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/8953035 8953035]
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[[Category: Protein complex]]
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[[Category: Arnold, J R.P.]]
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[[Category: Klug, A.]]
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[[Category: Murray, J B.]]
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[[Category: Scott, W G.]]
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[[Category: Stoddard, B L.]]
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[[Category: catalytic rna]]
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[[Category: loop]]
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[[Category: rna hammerhead ribozyme]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 15:43:51 2008''
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Current revision

CAPTURING THE STRUCTURE OF A CATALYTIC RNA INTERMEDIATE: THE HAMMERHEAD RIBOZYME

PDB ID 299d

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