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| | ==Crystal structure of the Get5 ubiquitin-like domain== | | ==Crystal structure of the Get5 ubiquitin-like domain== |
| - | <StructureSection load='4goc' size='340' side='right' caption='[[4goc]], [[Resolution|resolution]] 2.40Å' scene=''> | + | <StructureSection load='4goc' size='340' side='right'caption='[[4goc]], [[Resolution|resolution]] 2.40Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[4goc]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae_s288c Saccharomyces cerevisiae s288c]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4GOC OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4GOC FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[4goc]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Saccharomyces_cerevisiae_S288C Saccharomyces cerevisiae S288C]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4GOC OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4GOC FirstGlance]. <br> |
| - | </td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[4god|4god]], [[4goe|4goe]], [[4gof|4gof]]</td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.4Å</td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">GET5, MDY2, TMA24, YOL111C ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=559292 Saccharomyces cerevisiae S288c])</td></tr>
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4goc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4goc OCA], [https://pdbe.org/4goc PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4goc RCSB], [https://www.ebi.ac.uk/pdbsum/4goc PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4goc ProSAT]</span></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4goc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4goc OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4goc RCSB], [http://www.ebi.ac.uk/pdbsum/4goc PDBsum]</span></td></tr> | + | |
| | </table> | | </table> |
| | == Function == | | == Function == |
| - | [[http://www.uniprot.org/uniprot/MDY2_YEAST MDY2_YEAST]] Required for efficient mating. Involved in the production of alpha-factor, the KAR9 and TUB1 location to the shmoo tip and nuclear migration into pheromone-induced shmoos.<ref>PMID:10514570</ref> <ref>PMID:16390866</ref> | + | [https://www.uniprot.org/uniprot/MDY2_YEAST MDY2_YEAST] Required for efficient mating. Involved in the production of alpha-factor, the KAR9 and TUB1 location to the shmoo tip and nuclear migration into pheromone-induced shmoos.<ref>PMID:10514570</ref> <ref>PMID:16390866</ref> |
| - | <div style="background-color:#fffaf0;">
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| - | == Publication Abstract from PubMed ==
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| - | In the cytoplasm, the correct delivery of membrane proteins is an essential and highly regulated process. The posttranslational targeting of the important tail-anchor membrane (TA) proteins has recently been under intense investigation. A specialized pathway, called the guided entry of TA proteins (GET) pathway in yeast and the transmembrane domain recognition complex (TRC) pathway in vertebrates, recognizes endoplasmic-reticulum-targeted TA proteins and delivers them through a complex series of handoffs. An early step is the formation of a complex between Sgt2/SGTA, a cochaperone with a presumed ubiquitin-like-binding domain (UBD), and Get5/UBL4A, a ubiquitin-like domain (UBL)-containing protein. We structurally characterize this UBD/UBL interaction for both yeast and human proteins. This characterization is supported by biophysical studies that demonstrate that complex formation is mediated by electrostatics, generating an interface that has high-affinity with rapid kinetics. In total, this work provides a refined model of the interplay of Sgt2 homologs in TA targeting.
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| - | Structures of the Sgt2/SGTA Dimerization Domain with the Get5/UBL4A UBL Domain Reveal an Interaction that Forms a Conserved Dynamic Interface.,Chartron JW, Vandervelde DG, Clemons WM Jr Cell Rep. 2012 Nov 7. pii: S2211-1247(12)00346-4. doi:, 10.1016/j.celrep.2012.10.010. PMID:23142665<ref>PMID:23142665</ref>
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| - | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
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| - | </div>
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| | == References == | | == References == |
| | <references/> | | <references/> |
| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Saccharomyces cerevisiae s288c]] | + | [[Category: Large Structures]] |
| - | [[Category: Chartron, J W]] | + | [[Category: Saccharomyces cerevisiae S288C]] |
| - | [[Category: Clemons, W M]] | + | [[Category: Chartron JW]] |
| - | [[Category: VanderVelde, D G]] | + | [[Category: Clemons Jr WM]] |
| - | [[Category: Protein binding]] | + | [[Category: VanderVelde DG]] |
| - | [[Category: Protein-protein interaction]]
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| - | [[Category: Sgt2]]
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| - | [[Category: Ubiquitin-like domain]]
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