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| - | ==Crystal structure of carboxypeptidase 1 from Thermus thermophilus==
| + | #REDIRECT [[5wvu]] This PDB entry is obsolete and replaced by 5wvu |
| - | <StructureSection load='1wgz' size='340' side='right' caption='[[1wgz]], [[Resolution|resolution]] 2.60Å' scene=''>
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| - | == Structural highlights ==
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| - | <table><tr><td colspan='2'>[[1wgz]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Thermus_thermophilus Thermus thermophilus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1WGZ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1WGZ FirstGlance]. <br>
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| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
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| - | <tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Choriolysin_L Choriolysin L], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.24.66 3.4.24.66] </span></td></tr>
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| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1wgz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1wgz OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1wgz RCSB], [http://www.ebi.ac.uk/pdbsum/1wgz PDBsum], [http://www.topsan.org/Proteins/RSGI/1wgz TOPSAN]</span></td></tr>
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| - | </table>
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| - | == Function ==
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| - | [[http://www.uniprot.org/uniprot/Q5SLM3_THET8 Q5SLM3_THET8]] Broad specificity carboxypetidase that releases amino acids sequentially from the C-terminus, including neutral, aromatic, polar and basic residues, but not Pro. Has lower activity with substrates ending with Gly or Glu.
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| - | == Evolutionary Conservation ==
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| - | [[Image:Consurf_key_small.gif|200px|right]]
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| - | Check<jmol>
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| - | <jmolCheckbox>
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| - | <scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/wg/1wgz_consurf.spt"</scriptWhenChecked>
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| - | <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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| - | <text>to colour the structure by Evolutionary Conservation</text>
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| - | </jmolCheckbox>
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| - | </jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
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| - | <div style="clear:both"></div>
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| - | <div style="background-color:#fffaf0;">
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| - | == Publication Abstract from PubMed ==
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| - | Carboxypeptidase 1 from the thermophilic eubacterium Thermus thermophilus (TthCP1, 58 kDa), a member of the M32 family of metallocarboxypeptidases, was crystallized by the sitting-drop vapour-diffusion method using PEG 8000 as the precipitant. The crystals diffracted X-rays to beyond 2.6 A resolution using a synchrotron-radiation source. The crystals belonged to the orthorhombic space group C222(1), with unit-cell parameters a = 171.0, b = 231.6, c = 124.9 A. The crystal contains three molecules in an asymmetric unit (VM = 2.11 A3 Da(-1)) and has a solvent content of 61.5%.
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| - | Crystallization and preliminary X-ray analysis of carboxypeptidase 1 from Thermus thermophilus.,Nagata K, Tsutsui S, Lee WC, Ito K, Kamo M, Inoue Y, Tanokura M Acta Crystallogr D Biol Crystallogr. 2004 Aug;60(Pt 8):1445-6. Epub 2004, Jul 21. PMID:15272172<ref>PMID:15272172</ref>
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| - | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
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| - | </div>
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| - | ==See Also==
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| - | *[[Carboxypeptidase|Carboxypeptidase]]
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| - | == References ==
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| - | <references/>
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| - | __TOC__
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| - | </StructureSection>
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| - | [[Category: Choriolysin L]]
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| - | [[Category: Thermus thermophilus]]
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| - | [[Category: Inoue, Y]]
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| - | [[Category: Ito, K]]
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| - | [[Category: Kuramitsu, S]]
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| - | [[Category: Lee, W C]]
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| - | [[Category: Miyazono, K]]
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| - | [[Category: Nagata, K]]
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| - | [[Category: Structural genomic]]
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| - | [[Category: Tanokura, M]]
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| - | [[Category: Tsutsui, S]]
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| - | [[Category: Hydrolase]]
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| - | [[Category: Neurolysin-like carboxypeptidase]]
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| - | [[Category: Rsgi]]
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