2a5x

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[[Image:2a5x.gif|left|200px]]
 
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{{Structure
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==Crystal Structure of a Cross-linked Actin Dimer==
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|PDB= 2a5x |SIZE=350|CAPTION= <scene name='initialview01'>2a5x</scene>, resolution 2.49&Aring;
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<StructureSection load='2a5x' size='340' side='right'caption='[[2a5x]], [[Resolution|resolution]] 2.49&Aring;' scene=''>
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|SITE=
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== Structural highlights ==
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|LIGAND= <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=ANP:PHOSPHOAMINOPHOSPHONIC+ACID-ADENYLATE+ESTER'>ANP</scene>, <scene name='pdbligand=LAR:LATRUNCULIN+A'>LAR</scene>, <scene name='pdbligand=NSB:N,N,N-TRIMETHYL-3-SULFOPROPAN-1-AMINIUM'>NSB</scene> and <scene name='pdbligand=MPD:(4S)-2-METHYL-2,4-PENTANEDIOL'>MPD</scene>
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<table><tr><td colspan='2'>[[2a5x]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Oryctolagus_cuniculus Oryctolagus cuniculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2A5X OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2A5X FirstGlance]. <br>
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|ACTIVITY=
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.49&#8491;</td></tr>
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|GENE=
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ANP:PHOSPHOAMINOPHOSPHONIC+ACID-ADENYLATE+ESTER'>ANP</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=LAR:LATRUNCULIN+A'>LAR</scene>, <scene name='pdbligand=MPD:(4S)-2-METHYL-2,4-PENTANEDIOL'>MPD</scene>, <scene name='pdbligand=NSB:N,N,N-TRIMETHYL-3-SULFOPROPAN-1-AMINIUM'>NSB</scene></td></tr>
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}}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2a5x FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2a5x OCA], [https://pdbe.org/2a5x PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2a5x RCSB], [https://www.ebi.ac.uk/pdbsum/2a5x PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2a5x ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/ACTS_RABIT ACTS_RABIT] Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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The 2.5-A resolution crystal structure is reported for an actin dimer, composed of two protomers cross-linked along the longitudinal (or vertical) direction of the F-actin filament. The crystal structure provides an atomic resolution view of a molecular interface between actin protomers, which we argue represents a near-native interaction in the F-actin filament. The interaction involves subdomains 3 and 4 from distinct protomers. The atomic positions in the interface visualized differ by 5-10 A from those suggested by previous models of F-actin. Such differences fall within the range of uncertainties allowed by the fiber diffraction and electron microscopy methods on which previous models have been based. In the crystal, the translational arrangement of protomers lacks the slow twist found in native filaments. A plausible model of F-actin can be constructed by reintroducing the known filament twist, without disturbing significantly the interface observed in the actin dimer crystal.
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'''Crystal Structure of a Cross-linked Actin Dimer'''
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The crystal structure of a cross-linked actin dimer suggests a detailed molecular interface in F-actin.,Kudryashov DS, Sawaya MR, Adisetiyo H, Norcross T, Hegyi G, Reisler E, Yeates TO Proc Natl Acad Sci U S A. 2005 Sep 13;102(37):13105-10. Epub 2005 Sep 1. PMID:16141336<ref>PMID:16141336</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 2a5x" style="background-color:#fffaf0;"></div>
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==Overview==
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==See Also==
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The 2.5-A resolution crystal structure is reported for an actin dimer, composed of two protomers cross-linked along the longitudinal (or vertical) direction of the F-actin filament. The crystal structure provides an atomic resolution view of a molecular interface between actin protomers, which we argue represents a near-native interaction in the F-actin filament. The interaction involves subdomains 3 and 4 from distinct protomers. The atomic positions in the interface visualized differ by 5-10 A from those suggested by previous models of F-actin. Such differences fall within the range of uncertainties allowed by the fiber diffraction and electron microscopy methods on which previous models have been based. In the crystal, the translational arrangement of protomers lacks the slow twist found in native filaments. A plausible model of F-actin can be constructed by reintroducing the known filament twist, without disturbing significantly the interface observed in the actin dimer crystal.
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*[[Actin 3D structures|Actin 3D structures]]
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== References ==
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==About this Structure==
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<references/>
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2A5X is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Oryctolagus_cuniculus Oryctolagus cuniculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2A5X OCA].
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__TOC__
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</StructureSection>
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==Reference==
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[[Category: Large Structures]]
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The crystal structure of a cross-linked actin dimer suggests a detailed molecular interface in F-actin., Kudryashov DS, Sawaya MR, Adisetiyo H, Norcross T, Hegyi G, Reisler E, Yeates TO, Proc Natl Acad Sci U S A. 2005 Sep 13;102(37):13105-10. Epub 2005 Sep 1. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/16141336 16141336]
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[[Category: Oryctolagus cuniculus]]
[[Category: Oryctolagus cuniculus]]
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[[Category: Single protein]]
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[[Category: Adisetiyo H]]
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[[Category: Adisetiyo, H.]]
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[[Category: Hegyi G]]
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[[Category: Hegyi, G.]]
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[[Category: Kudryashov DS]]
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[[Category: Kudryashov, D S.]]
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[[Category: Norcross T]]
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[[Category: Norcross, T.]]
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[[Category: Reisler E]]
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[[Category: Reisler, E.]]
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[[Category: Sawaya MR]]
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[[Category: Sawaya, M R.]]
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[[Category: Yeates TO]]
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[[Category: Yeates, T O.]]
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[[Category: ANP]]
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[[Category: CA]]
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[[Category: LAR]]
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[[Category: MPD]]
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[[Category: NSB]]
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[[Category: muscle protein]]
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[[Category: nucleotide-binding]]
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[[Category: structural protein]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 15:45:47 2008''
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Current revision

Crystal Structure of a Cross-linked Actin Dimer

PDB ID 2a5x

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