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2a65

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[[Image:2a65.gif|left|200px]]
 
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{{Structure
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==Crystal structure of LEUTAA, a bacterial homolog of Na+/Cl--dependent neurotransmitter transporters==
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|PDB= 2a65 |SIZE=350|CAPTION= <scene name='initialview01'>2a65</scene>, resolution 1.650&Aring;
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<StructureSection load='2a65' size='340' side='right'caption='[[2a65]], [[Resolution|resolution]] 1.65&Aring;' scene=''>
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|SITE=
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== Structural highlights ==
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|LIGAND= <scene name='pdbligand=BOG:B-OCTYLGLUCOSIDE'>BOG</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene> and <scene name='pdbligand=LEU:LEUCINE'>LEU</scene>
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<table><tr><td colspan='2'>[[2a65]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Aquifex_aeolicus_VF5 Aquifex aeolicus VF5]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2A65 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2A65 FirstGlance]. <br>
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|ACTIVITY=
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.65&#8491;</td></tr>
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|GENE=
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BOG:B-OCTYLGLUCOSIDE'>BOG</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=LEU:LEUCINE'>LEU</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene></td></tr>
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}}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2a65 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2a65 OCA], [https://pdbe.org/2a65 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2a65 RCSB], [https://www.ebi.ac.uk/pdbsum/2a65 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2a65 ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/O67854_AQUAE O67854_AQUAE]
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/a6/2a65_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2a65 ConSurf].
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<div style="clear:both"></div>
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'''Crystal structure of LEUTAA, a bacterial homolog of Na+/Cl--dependent neurotransmitter transporters'''
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==See Also==
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*[[Symporter 3D structures|Symporter 3D structures]]
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__TOC__
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==Overview==
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</StructureSection>
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Na+/Cl--dependent transporters terminate synaptic transmission by using electrochemical gradients to drive the uptake of neurotransmitters, including the biogenic amines, from the synapse to the cytoplasm of neurons and glia. These transporters are the targets of therapeutic and illicit compounds, and their dysfunction has been implicated in multiple diseases of the nervous system. Here we present the crystal structure of a bacterial homologue of these transporters from Aquifex aeolicus, in complex with its substrate, leucine, and two sodium ions. The protein core consists of the first ten of twelve transmembrane segments, with segments 1-5 related to 6-10 by a pseudo-two-fold axis in the membrane plane. Leucine and the sodium ions are bound within the protein core, halfway across the membrane bilayer, in an occluded site devoid of water. The leucine and ion binding sites are defined by partially unwound transmembrane helices, with main-chain atoms and helix dipoles having key roles in substrate and ion binding. The structure reveals the architecture of this important class of transporter, illuminates the determinants of substrate binding and ion selectivity, and defines the external and internal gates.
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[[Category: Aquifex aeolicus VF5]]
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[[Category: Large Structures]]
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==About this Structure==
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[[Category: Gouaux E]]
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2A65 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Aquifex_aeolicus_vf5 Aquifex aeolicus vf5]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2A65 OCA].
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[[Category: Jin Y]]
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[[Category: Kawate T]]
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==Reference==
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[[Category: Singh SK]]
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Crystal structure of a bacterial homologue of Na+/Cl--dependent neurotransmitter transporters., Yamashita A, Singh SK, Kawate T, Jin Y, Gouaux E, Nature. 2005 Sep 8;437(7056):215-23. Epub 2005 Jul 24. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/16041361 16041361]
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[[Category: Yamashita A]]
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[[Category: Aquifex aeolicus vf5]]
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[[Category: Single protein]]
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[[Category: Gouaux, E.]]
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[[Category: Jin, Y.]]
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[[Category: Kawate, T.]]
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[[Category: Singh, S K.]]
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[[Category: Yamashita, A.]]
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[[Category: BOG]]
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[[Category: CL]]
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[[Category: LEU]]
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[[Category: NA]]
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[[Category: membrane protein]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 15:45:49 2008''
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Current revision

Crystal structure of LEUTAA, a bacterial homolog of Na+/Cl--dependent neurotransmitter transporters

PDB ID 2a65

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