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| | ==Structure of the parainfluenza virus 5 (PIV5) hemagglutinin-neuraminidase (HN) stalk domain== | | ==Structure of the parainfluenza virus 5 (PIV5) hemagglutinin-neuraminidase (HN) stalk domain== |
| - | <StructureSection load='3tsi' size='340' side='right' caption='[[3tsi]], [[Resolution|resolution]] 2.65Å' scene=''> | + | <StructureSection load='3tsi' size='340' side='right'caption='[[3tsi]], [[Resolution|resolution]] 2.65Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[3tsi]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Parainfluenza_virus_5 Parainfluenza virus 5]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3TSI OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3TSI FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[3tsi]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Simian_virus_5_(strain_W3) Simian virus 5 (strain W3)]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3TSI OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3TSI FirstGlance]. <br> |
| - | </td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">HN ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=11207 Parainfluenza virus 5])</td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.651Å</td></tr> |
| - | <tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Exo-alpha-sialidase Exo-alpha-sialidase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.18 3.2.1.18] </span></td></tr>
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3tsi FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3tsi OCA], [https://pdbe.org/3tsi PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3tsi RCSB], [https://www.ebi.ac.uk/pdbsum/3tsi PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3tsi ProSAT]</span></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3tsi FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3tsi OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3tsi RCSB], [http://www.ebi.ac.uk/pdbsum/3tsi PDBsum]</span></td></tr> | + | |
| | </table> | | </table> |
| | == Function == | | == Function == |
| - | [[http://www.uniprot.org/uniprot/HN_SV5 HN_SV5]] Attaches the virus to sialic acid-containing cell receptors and thereby initiating infection. Binding of HN protein to the receptor induces a conformational change that allows the F protein to trigger virion/cell membranes fusion (By similarity). Neuraminidase activity ensures the efficient spread of the virus by dissociating the mature virions from the neuraminic acid containing glycoproteins (By similarity). | + | [https://www.uniprot.org/uniprot/HN_PIV5 HN_PIV5] Attaches the virus to sialic acid-containing cell receptors and thereby initiating infection. Binding of HN protein to the receptor induces a conformational change that allows the F protein to trigger virion/cell membranes fusion (By similarity). Neuraminidase activity ensures the efficient spread of the virus by dissociating the mature virions from the neuraminic acid containing glycoproteins. |
| - | <div style="background-color:#fffaf0;">
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| - | == Publication Abstract from PubMed ==
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| - | Paramyxovirus entry into cells requires the fusion protein (F) and a receptor binding protein (HN, H or G). The multifunctional hemagglutinin-neuraminidase (HN) protein of some paramyxoviruses, besides functioning as the receptor (sialic acid) binding protein (hemagglutinin activity) and the receptor-destroying protein (neuraminidase activity), enhances fusion protein (F) activity, presumably by lowering the activation energy required for F to mediate fusion of viral and cellular membranes. Before or upon receptor binding by the HN globular head, F is believed to interact with the HN stalk. Unfortunately until recently none of the receptor binding protein crystal structures have shown electron density for the stalk domain. Parainfluenza virus 5 (PIV5) HN exists as a non-covalent dimer-of-dimers on the surface of cells, linked by a single disulfide bond in the stalk. Here we present the crystal structure of the PIV5-HN stalk domain at a resolution of 2.65 A revealing a four-helix bundle (4HB) with an upper (N-terminal) straight region and a lower (C-terminal) supercoiled part. The hydrophobic core residues are a mix of an 11-mer repeat and a 3-4 heptad repeat. To functionally characterize the role of the HN stalk in F-interactions and fusion, we designed mutants along the PIV5-HN stalk that are N-glycosylated to physically disrupt F-HN interactions. By extensive study of receptor binding, neuraminidase activity, oligomerization and fusion promoting functions of the mutant proteins we found a correlation between the position of the N-glycosylation mutants on the stalk structure and their neuraminidase activities as well as their abilities to promote fusion.
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| - | Structure and mutagenesis of the parainfluenza virus 5 hemagglutinin-neuraminidase stalk domain reveals a four-helix bundle and the role of the stalk in fusion promotion.,Bose S, Welch BD, Kors CA, Yuan P, Jardetzky TS, Lamb RA J Virol. 2011 Oct 12. PMID:21994464<ref>PMID:21994464</ref>
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| - | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
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| - | </div>
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| | ==See Also== | | ==See Also== |
| - | *[[Hemagglutinin|Hemagglutinin]] | + | *[[Hemagglutinin 3D structures|Hemagglutinin 3D structures]] |
| - | == References ==
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| - | <references/>
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| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Exo-alpha-sialidase]] | + | [[Category: Large Structures]] |
| - | [[Category: Parainfluenza virus 5]]
| + | [[Category: Bose S]] |
| - | [[Category: Bose, S]] | + | [[Category: Jardetzky TS]] |
| - | [[Category: Jardetzky, T S]] | + | [[Category: Kors CA]] |
| - | [[Category: Kors, C A]] | + | [[Category: Lamb RA]] |
| - | [[Category: Lamb, R A]] | + | [[Category: Welch BD]] |
| - | [[Category: Welch, B D]] | + | [[Category: Yuan P]] |
| - | [[Category: Yuan, P]] | + | |
| - | [[Category: 11-mer hydrophobic repeat]]
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| - | [[Category: Ectodomain]]
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| - | [[Category: Four stranded coiled coil]]
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| - | [[Category: Four-helix bundle]]
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| - | [[Category: Receptor binding protein]]
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| - | [[Category: Viral protein]]
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