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| | ==X-Ray Crystal Structure of the passenger domain of Plasmid encoded toxin, an Autrotansporter Enterotoxin from enteroaggregative Escherichia coli (EAEC)== | | ==X-Ray Crystal Structure of the passenger domain of Plasmid encoded toxin, an Autrotansporter Enterotoxin from enteroaggregative Escherichia coli (EAEC)== |
| - | <StructureSection load='4om9' size='340' side='right' caption='[[4om9]], [[Resolution|resolution]] 2.30Å' scene=''> | + | <StructureSection load='4om9' size='340' side='right'caption='[[4om9]], [[Resolution|resolution]] 2.30Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[4om9]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Eaec_042 Eaec 042]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4OM9 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4OM9 FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[4om9]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli_042 Escherichia coli 042]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4OM9 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4OM9 FirstGlance]. <br> |
| - | </td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[3sze|3sze]]</td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.3Å</td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">EC042_pAA035, pet ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=216592 EAEC 042])</td></tr>
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4om9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4om9 OCA], [https://pdbe.org/4om9 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4om9 RCSB], [https://www.ebi.ac.uk/pdbsum/4om9 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4om9 ProSAT]</span></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4om9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4om9 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4om9 RCSB], [http://www.ebi.ac.uk/pdbsum/4om9 PDBsum]</span></td></tr> | + | |
| | </table> | | </table> |
| | == Function == | | == Function == |
| - | [[http://www.uniprot.org/uniprot/PET_ECO44 PET_ECO44]] Serine protease with enterotoxic and cytotoxic activity. Internalization into the host cell is required for the induction of cytopathic effects. However, the serine activity is not necessary for secretion and internalization into the host cell.<ref>PMID:9632580</ref> <ref>PMID:10225873</ref> <ref>PMID:10496914</ref> <ref>PMID:11160002</ref> | + | [https://www.uniprot.org/uniprot/PET_ECO44 PET_ECO44] Serine protease with enterotoxic and cytotoxic activity. Internalization into the host cell is required for the induction of cytopathic effects. However, the serine activity is not necessary for secretion and internalization into the host cell.<ref>PMID:9632580</ref> <ref>PMID:10225873</ref> <ref>PMID:10496914</ref> <ref>PMID:11160002</ref> |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> |
| | </div> | | </div> |
| | + | <div class="pdbe-citations 4om9" style="background-color:#fffaf0;"></div> |
| | == References == | | == References == |
| | <references/> | | <references/> |
| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Eaec 042]] | + | [[Category: Escherichia coli 042]] |
| - | [[Category: Eslava-Campos, C A]] | + | [[Category: Large Structures]] |
| - | [[Category: Fromme, P]] | + | [[Category: Arreguin-Espinosa de Los Monteros RA]] |
| - | [[Category: Fromme, R]] | + | [[Category: Eslava-Campos CA]] |
| - | [[Category: Hernandez-Chinas, U]] | + | [[Category: Fromme P]] |
| - | [[Category: Mendoza-Hernandez, G]] | + | [[Category: Fromme R]] |
| - | [[Category: Meza-Aguilar, J D]] | + | [[Category: Hernandez-Chinas U]] |
| - | [[Category: Monteros, R A.Arreguin-Espinosa de Los]]
| + | [[Category: Mendoza-Hernandez G]] |
| - | [[Category: Torres-Larios, A]] | + | [[Category: Meza-Aguilar JD]] |
| - | [[Category: Alpha-fodrin]]
| + | [[Category: Torres-Larios A]] |
| - | [[Category: Beta-helix]]
| + | |
| - | [[Category: Eaec]]
| + | |
| - | [[Category: Hydrolase]]
| + | |
| - | [[Category: Peptidase]]
| + | |
| Structural highlights
Function
PET_ECO44 Serine protease with enterotoxic and cytotoxic activity. Internalization into the host cell is required for the induction of cytopathic effects. However, the serine activity is not necessary for secretion and internalization into the host cell.[1] [2] [3] [4]
Publication Abstract from PubMed
Autotransporters (ATs) represent a superfamily of proteins produced by a variety of pathogenic bacteria, which include the pathogenic groups of Escherichia coli (E. coli) associated with gastrointestinal and urinary tract infections. We present the first X-ray structure of the passenger domain from the Plasmid-encoded toxin (Pet) a 100kDa protein at 2.3A resolution which is a cause of acute diarrhea in both developing and industrialized countries. Pet is a cytoskeleton-altering toxin that induces loss of actin stress fibers. While Pet (pdb code: 4OM9) shows only a sequence identity of 50% compared to the closest related protein sequence, extracellular serine protease plasmid (EspP) the structural features of both proteins are conserved. A closer structural look reveals that Pet contains a beta-pleaded sheet at the sequence region of residues 181-190, the corresponding structural domain in EspP consists of a coiled loop. Secondary, the Pet passenger domain features a more pronounced beta sheet between residues 135 and 143 compared to the structure of EspP.
X-ray crystal structure of the passenger domain of plasmid encoded toxin(Pet), an autotransporter enterotoxin from enteroaggregative Escherichia coli (EAEC).,Domingo Meza-Aguilar J, Fromme P, Torres-Larios A, Mendoza-Hernandez G, Hernandez-Chinas U, Arreguin-Espinosa de Los Monteros RA, Eslava Campos CA, Fromme R Biochem Biophys Res Commun. 2014 Feb 12. pii: S0006-291X(14)00268-X. doi:, 10.1016/j.bbrc.2014.02.016. PMID:24530907[5]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Eslava C, Navarro-Garcia F, Czeczulin JR, Henderson IR, Cravioto A, Nataro JP. Pet, an autotransporter enterotoxin from enteroaggregative Escherichia coli. Infect Immun. 1998 Jul;66(7):3155-63. PMID:9632580
- ↑ Navarro-Garcia F, Sears C, Eslava C, Cravioto A, Nataro JP. Cytoskeletal effects induced by pet, the serine protease enterotoxin of enteroaggregative Escherichia coli. Infect Immun. 1999 May;67(5):2184-92. PMID:10225873
- ↑ Henderson IR, Hicks S, Navarro-Garcia F, Elias WP, Philips AD, Nataro JP. Involvement of the enteroaggregative Escherichia coli plasmid-encoded toxin in causing human intestinal damage. Infect Immun. 1999 Oct;67(10):5338-44. PMID:10496914
- ↑ Navarro-Garcia F, Canizalez-Roman A, Luna J, Sears C, Nataro JP. Plasmid-encoded toxin of enteroaggregative Escherichia coli is internalized by epithelial cells. Infect Immun. 2001 Feb;69(2):1053-60. PMID:11160002 doi:http://dx.doi.org/10.1128/IAI.69.2.1053-1060.2001
- ↑ Domingo Meza-Aguilar J, Fromme P, Torres-Larios A, Mendoza-Hernandez G, Hernandez-Chinas U, Arreguin-Espinosa de Los Monteros RA, Eslava Campos CA, Fromme R. X-ray crystal structure of the passenger domain of plasmid encoded toxin(Pet), an autotransporter enterotoxin from enteroaggregative Escherichia coli (EAEC). Biochem Biophys Res Commun. 2014 Feb 12. pii: S0006-291X(14)00268-X. doi:, 10.1016/j.bbrc.2014.02.016. PMID:24530907 doi:http://dx.doi.org/10.1016/j.bbrc.2014.02.016
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