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| - | [[Image:2ana.jpg|left|200px]] | |
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| - | {{Structure
| + | ==THE CRYSTAL STRUCTURE OF D(G-G-G-G-C-C-C-C). A MODEL FOR POLY(DG).POLY(DC)== |
| - | |PDB= 2ana |SIZE=350|CAPTION= <scene name='initialview01'>2ana</scene>, resolution 2.500Å
| + | <StructureSection load='2ana' size='340' side='right'caption='[[2ana]], [[Resolution|resolution]] 2.50Å' scene=''> |
| - | |SITE=
| + | == Structural highlights == |
| - | |LIGAND=
| + | <table><tr><td colspan='2'>[[2ana]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2ANA OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2ANA FirstGlance]. <br> |
| - | |ACTIVITY=
| + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.5Å</td></tr> |
| - | |GENE=
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2ana FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2ana OCA], [https://pdbe.org/2ana PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2ana RCSB], [https://www.ebi.ac.uk/pdbsum/2ana PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2ana ProSAT]</span></td></tr> |
| - | }}
| + | </table> |
| - | | + | __TOC__ |
| - | '''THE CRYSTAL STRUCTURE OF D(G-G-G-G-C-C-C-C). A MODEL FOR POLY(DG).POLY(DC)'''
| + | </StructureSection> |
| - | | + | [[Category: Large Structures]] |
| - | | + | [[Category: Brown T]] |
| - | ==Overview== | + | [[Category: Kennard O]] |
| - | The structure of the DNA oligomer d(G-G-G-G-C-C-C-C) has been determined at a resolution of 2.5 A by single-crystal X-ray methods. There are two strands in the asymmetric unit, and these coil about each other to form a right-handed double-helix of the A-type with Watson-Crick hydrogen bonds between base-pairs. The helix has a shallow minor groove and a deep, water-filled major groove; almost all exposed functional groups on the DNA are hydrated, and 106 ordered solvent molecules have been found. The two d(G-G-G-G).d(C-C-C-C) segments in the octamer exhibit similar and uniform structures, but there is a slight discontinuity at the GpC step between them. A recurring feature of the structure is the overlap of adjacent guanine bases in each GpG step, with the five-membered ring of one guanine stacking on the six-membered ring of its neighbour. There is little or no overlap between adjacent cytosine rings. Conformational parameters for these GpG steps are compared with those from other single-crystal X-ray analyses. In general, GpG steps exhibit high slide, low roll and variable twist. Models for poly(dG).poly(dC) were generated by applying a simple rotation and translation to each of the unmodified d(G-G-G-G).d(C-C-C-C) units. Detailed features of these models are shown to be compatible with various assays of poly(dG).poly(dC) in solution, and are useful in understanding the polymorphic behaviour of this sequence under a variety of experimental conditions.
| + | [[Category: McCall M]] |
| - | | + | |
| - | ==About this Structure== | + | |
| - | 2ANA is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/ ]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2ANA OCA].
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| - | ==Reference==
| + | |
| - | The crystal structure of d(G-G-G-G-C-C-C-C). A model for poly(dG).poly(dC)., McCall M, Brown T, Kennard O, J Mol Biol. 1985 Jun 5;183(3):385-96. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/4020865 4020865]
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| - | [[Category: Protein complex]] | + | |
| - | [[Category: Brown, T.]] | + | |
| - | [[Category: Kennard, O.]] | + | |
| - | [[Category: McCall, M.]] | + | |
| - | [[Category: a-dna]]
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| - | [[Category: double helix]]
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| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 15:51:38 2008''
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