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3ryg

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==128 hours neutron structure of perdeuterated rubredoxin==
==128 hours neutron structure of perdeuterated rubredoxin==
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<StructureSection load='3ryg' size='340' side='right' caption='[[3ryg]], [[Resolution|resolution]] 1.75&Aring;' scene=''>
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<StructureSection load='3ryg' size='340' side='right'caption='[[3ryg]], [[Resolution|resolution]] 1.75&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
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<table><tr><td colspan='2'>[[3ryg]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Pyrococcus_furiosus Pyrococcus furiosus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3RYG OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3RYG FirstGlance]. <br>
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<table><tr><td colspan='2'>[[3ryg]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Pyrococcus_furiosus_DSM_3638 Pyrococcus furiosus DSM 3638]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3RYG OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3RYG FirstGlance]. <br>
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</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=DOD:DEUTERATED+WATER'>DOD</scene>, <scene name='pdbligand=FE:FE+(III)+ION'>FE</scene></td></tr>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Neutron Diffraction, [[Resolution|Resolution]] 1.75&#8491;</td></tr>
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<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[3kyx|3kyx]], [[3rz6|3rz6]], [[3rzr|3rzr]], [[3rzt|3rzt]], [[3ss2|3ss2]]</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=DOD:DEUTERATED+WATER'>DOD</scene>, <scene name='pdbligand=FE:FE+(III)+ION'>FE</scene></td></tr>
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<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">PF1282, rub ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=2261 Pyrococcus furiosus])</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3ryg FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3ryg OCA], [https://pdbe.org/3ryg PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3ryg RCSB], [https://www.ebi.ac.uk/pdbsum/3ryg PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3ryg ProSAT]</span></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3ryg FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3ryg OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3ryg RCSB], [http://www.ebi.ac.uk/pdbsum/3ryg PDBsum]</span></td></tr>
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</table>
</table>
== Function ==
== Function ==
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[[http://www.uniprot.org/uniprot/RUBR_PYRFU RUBR_PYRFU]] Rubredoxin is a small nonheme, iron protein lacking acid-labile sulfide. Its single Fe, chelated to 4 Cys, functions as an electron acceptor and may also stabilize the conformation of the molecule.
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[https://www.uniprot.org/uniprot/RUBR_PYRFU RUBR_PYRFU] Rubredoxin is a small nonheme, iron protein lacking acid-labile sulfide. Its single Fe, chelated to 4 Cys, functions as an electron acceptor and may also stabilize the conformation of the molecule.
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Neutron crystallography is a powerful technique for experimental visualization of the positions of light atoms, including hydrogen and its isotope deuterium. In recent years, structural biologists have shown increasing interest in the technique as it uniquely complements X-ray crystallographic data by revealing the positions of D atoms in macromolecules. With this regained interest, access to macromolecular neutron crystallography beamlines is becoming a limiting step. In this report, it is shown that a rapid data-collection strategy can be a valuable alternative to longer data-collection times in appropriate cases. Comparison of perdeuterated rubredoxin structures refined against neutron data sets collected over hours and up to 5 d shows that rapid neutron data collection in just 14 h is sufficient to provide the positions of 269 D atoms without ambiguity.
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Rapid visualization of hydrogen positions in protein neutron crystallographic structures.,Munshi P, Chung SL, Blakeley MP, Weiss KL, Myles DA, Meilleur F Acta Crystallogr D Biol Crystallogr. 2012 Jan;68(Pt 1):35-41. Epub 2011, Dec 9. PMID:22194331<ref>PMID:22194331</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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==See Also==
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</div>
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*[[Rubredoxin 3D structures|Rubredoxin 3D structures]]
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== References ==
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*[[Rubredoxin PDB structures|Rubredoxin PDB structures]]
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<references/>
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__TOC__
__TOC__
</StructureSection>
</StructureSection>
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[[Category: Pyrococcus furiosus]]
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[[Category: Large Structures]]
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[[Category: Blakeley, M P]]
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[[Category: Pyrococcus furiosus DSM 3638]]
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[[Category: Chung, C-L]]
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[[Category: Blakeley MP]]
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[[Category: Meilleur, F]]
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[[Category: Chung C-L]]
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[[Category: Munshi, P]]
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[[Category: Meilleur F]]
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[[Category: Myles, D A.A]]
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[[Category: Munshi P]]
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[[Category: Weiss, K L]]
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[[Category: Myles DAA]]
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[[Category: Electron transport]]
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[[Category: Weiss KL]]
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[[Category: Iron]]
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[[Category: Metal-binding]]
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[[Category: Transport]]
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Current revision

128 hours neutron structure of perdeuterated rubredoxin

PDB ID 3ryg

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