2w2h

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==STRUCTURAL BASIS OF TRANSCRIPTION ACTIVATION BY THE CYCLIN T1-TAT-TAR RNA COMPLEX FROM EIAV==
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<StructureSection load='2w2h' size='340' side='right' caption='[[2w2h]], [[Resolution|resolution]] 3.25&Aring;' scene=''>
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==Structural basis of transcription activation by the Cyclin T1-Tat-TAR RNA complex from EIAV==
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<StructureSection load='2w2h' size='340' side='right'caption='[[2w2h]], [[Resolution|resolution]] 3.25&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
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<table><tr><td colspan='2'>[[2w2h]] is a 6 chain structure with sequence from [http://en.wikipedia.org/wiki/Equine_infectious_anemia_virus Equine infectious anemia virus] and [http://en.wikipedia.org/wiki/Equus_caballus Equus caballus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2W2H OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2W2H FirstGlance]. <br>
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<table><tr><td colspan='2'>[[2w2h]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Equine_infectious_anemia_virus Equine infectious anemia virus] and [https://en.wikipedia.org/wiki/Equus_caballus Equus caballus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2W2H OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2W2H FirstGlance]. <br>
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</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene></td></tr>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3.25&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2w2h FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2w2h OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2w2h RCSB], [http://www.ebi.ac.uk/pdbsum/2w2h PDBsum]</span></td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2w2h FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2w2h OCA], [https://pdbe.org/2w2h PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2w2h RCSB], [https://www.ebi.ac.uk/pdbsum/2w2h PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2w2h ProSAT]</span></td></tr>
</table>
</table>
== Function ==
== Function ==
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[[http://www.uniprot.org/uniprot/TAT_EIAVY TAT_EIAVY]] Nuclear transcriptional activator of viral gene expression, that is essential for viral transcription from the LTR promoter and replication. Acts as a sequence-specific molecular adapter, directing components of the cellular transcription machinery to the viral RNA to enhance transcription by the RNA polymerase II (RNA pol II) complex, thereby increasing the level of full-length transcripts. Tat associates with the CCNT1/cyclin-T1 component of the P-TEFb complex (CDK9 and CCNT1), which promotes RNA chain elongation. This binding increases Tat's affinity for a branched hairpin structure at the 5'-end of all nascent viral mRNAs referred to as the transactivation responsive RNA element (TAR RNA). The CDK9 component of P-TEFb hyperphosphorylates the C-terminus of RNA Pol II that becomes stabilized and much more processive (Probable).
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[https://www.uniprot.org/uniprot/CCNT1_HORSE CCNT1_HORSE] Regulatory subunit of the cyclin-dependent kinase pair (CDK9/cyclin-T1) complex, also called positive transcription elongation factor B (P-TEFb), which facilitates the transition from abortive to productive elongation by phosphorylating the CTD (C-terminal domain) of the large subunit of RNA polymerase II (RNA Pol II). Required to activate the protein kinase activity of CDK9: acts by mediating formation of liquid-liquid phase separation (LLPS) that enhances binding of P-TEFb to the CTD of RNA Pol II.[UniProtKB:O60563]
== Evolutionary Conservation ==
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
Check<jmol>
<jmolCheckbox>
<jmolCheckbox>
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<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/w2/2w2h_consurf.spt"</scriptWhenChecked>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/w2/2w2h_consurf.spt"</scriptWhenChecked>
<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
<text>to colour the structure by Evolutionary Conservation</text>
<text>to colour the structure by Evolutionary Conservation</text>
</jmolCheckbox>
</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2w2h ConSurf].
<div style="clear:both"></div>
<div style="clear:both"></div>
<div style="background-color:#fffaf0;">
<div style="background-color:#fffaf0;">
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
</div>
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<div class="pdbe-citations 2w2h" style="background-color:#fffaf0;"></div>
==See Also==
==See Also==
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*[[Cyclin|Cyclin]]
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*[[Cyclin 3D structures|Cyclin 3D structures]]
*[[Tat protein|Tat protein]]
*[[Tat protein|Tat protein]]
== References ==
== References ==
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[[Category: Equine infectious anemia virus]]
[[Category: Equine infectious anemia virus]]
[[Category: Equus caballus]]
[[Category: Equus caballus]]
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[[Category: Anand, K]]
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[[Category: Large Structures]]
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[[Category: Geyer, M]]
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[[Category: Anand K]]
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[[Category: Activator]]
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[[Category: Geyer M]]
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[[Category: Cell cycle]]
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[[Category: Cell division]]
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[[Category: Crystallization]]
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[[Category: Cyclin]]
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[[Category: Cyclin t1]]
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[[Category: Host-virus interaction]]
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[[Category: Nucleus]]
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[[Category: Rna-binding]]
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[[Category: Rna-binding protein]]
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[[Category: Tar]]
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[[Category: Tat]]
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[[Category: Transcription]]
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[[Category: Transcription regulation]]
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Current revision

Structural basis of transcription activation by the Cyclin T1-Tat-TAR RNA complex from EIAV

PDB ID 2w2h

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