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| | ==Crystal structure of the MukE-MukF complex== | | ==Crystal structure of the MukE-MukF complex== |
| - | <StructureSection load='3rpu' size='340' side='right' caption='[[3rpu]], [[Resolution|resolution]] 3.60Å' scene=''> | + | <StructureSection load='3rpu' size='340' side='right'caption='[[3rpu]], [[Resolution|resolution]] 3.60Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[3rpu]] is a 9 chain structure with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3RPU OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3RPU FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[3rpu]] is a 9 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli_K-12 Escherichia coli K-12]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3RPU OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3RPU FirstGlance]. <br> |
| - | </td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[3euh|3euh]], [[3euj|3euj]], [[3euk|3euk]], [[1t98|1t98]]</td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3.6Å</td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">b0922, JW0905, kicB, mukF, mukF (kicB) ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 Escherichia coli]), b0923, JW0906, kicA, mukE, MukE (kicA, ycbA, ycbA) ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 Escherichia coli])</td></tr>
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3rpu FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3rpu OCA], [https://pdbe.org/3rpu PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3rpu RCSB], [https://www.ebi.ac.uk/pdbsum/3rpu PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3rpu ProSAT]</span></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3rpu FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3rpu OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3rpu RCSB], [http://www.ebi.ac.uk/pdbsum/3rpu PDBsum]</span></td></tr> | + | |
| | </table> | | </table> |
| | == Function == | | == Function == |
| - | [[http://www.uniprot.org/uniprot/MUKF_ECOLI MUKF_ECOLI]] Involved in chromosome condensation, segregation and cell cycle progression. May participate in facilitating chromosome segregation by condensation DNA from both sides of a centrally located replisome during cell division. Not required for mini-F plasmid partitioning. Probably acts via its interaction with MukB and MukE. Overexpression results in anucleate cells. It has a calcium binding activity.<ref>PMID:8602138</ref> [[http://www.uniprot.org/uniprot/MUKE_ECOLI MUKE_ECOLI]] Involved in chromosome condensation, segregation and cell cycle progression. May participate in facilitating chromosome segregation by condensation DNA from both sides of a centrally located replisome during cell division. Probably acts via its interaction with MukB and MukF.<ref>PMID:8602138</ref> | + | [https://www.uniprot.org/uniprot/MUKF_ECOLI MUKF_ECOLI] Involved in chromosome condensation, segregation and cell cycle progression. May participate in facilitating chromosome segregation by condensation DNA from both sides of a centrally located replisome during cell division. Not required for mini-F plasmid partitioning. Probably acts via its interaction with MukB and MukE. Overexpression results in anucleate cells. It has a calcium binding activity.<ref>PMID:8602138</ref> |
| - | <div style="background-color:#fffaf0;">
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| - | == Publication Abstract from PubMed ==
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| - | The MukB-MukE-MukF protein complex is essential for chromosome condensation and segregation in Escherichia coli. The central component of this complex, the MukB protein, is related functionally and structurally to the ubiquitous SMC (structural maintenance of chromosomes) proteins. In a manner similar to SMC, MukB requires the association of two accessory proteins (MukE and MukF) for its function. MukF is a constitutive dimer that bridges the interaction between MukB and MukE. While MukB can condense DNA on its own, it requires MukF and MukE to ensure proper chromosome segregation. Here, we present a novel structure of the E. coli MukE-MukF complex, in which the intricate crystal packing interactions reveal an alternative MukE dimerization interface spanning both N- and C-terminal winged-helix domains of the protein. The structure also unveils additional cross-linking interactions between adjacent MukE-MukF complexes mediated by MukE. A variant of MukE encompassing point mutations on one of these surfaces does not affect assembly of the MukB-MukE-MukF complex and yet cannot restore the temperature sensitivity of the mukEColon, two colonskan strain, suggesting that this surface may mediate critical protein-protein interactions between MukB-MukE-MukF complexes. Since the dimerization interface of MukE overlaps with the region of the protein that interacts with MukB in the MukB-MukE-MukF complex, we suggest that competing MukB-MukE and MukE-MukE interactions may regulate the formation of higher-order structures of bacterial condensin.
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| - | The role of MukE in assembling a functional MukBEF complex.,Gloyd M, Ghirlando R, Guarne A J Mol Biol. 2011 Sep 30;412(4):578-90. Epub 2011 Aug 10. PMID:21855551<ref>PMID:21855551</ref>
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| - | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
| + | ==See Also== |
| - | </div>
| + | *[[Condensin|Condensin]] |
| | == References == | | == References == |
| | <references/> | | <references/> |
| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Escherichia coli]] | + | [[Category: Escherichia coli K-12]] |
| - | [[Category: Ghirlando, R]] | + | [[Category: Large Structures]] |
| - | [[Category: Gloyd, M]] | + | [[Category: Ghirlando R]] |
| - | [[Category: Guarne, A]] | + | [[Category: Gloyd M]] |
| - | [[Category: Cell cycle]] | + | [[Category: Guarne A]] |
| - | [[Category: Chromosome condensation and segregation]]
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| - | [[Category: Kleisin]]
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| - | [[Category: Mukb]]
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| - | [[Category: Smc-associated protein]]
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