|
|
| (2 intermediate revisions not shown.) |
| Line 1: |
Line 1: |
| | + | |
| | ==Structural characterization of the interaction of colicin A, colicin N, and TolB with the TolAIII translocon== | | ==Structural characterization of the interaction of colicin A, colicin N, and TolB with the TolAIII translocon== |
| - | <StructureSection load='3qdr' size='340' side='right' caption='[[3qdr]], [[Resolution|resolution]] 2.65Å' scene=''> | + | <StructureSection load='3qdr' size='340' side='right'caption='[[3qdr]], [[Resolution|resolution]] 2.65Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[3qdr]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Citrobacter_freundii Citrobacter freundii] and [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3QDR OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3QDR FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[3qdr]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Citrobacter_freundii Citrobacter freundii] and [https://en.wikipedia.org/wiki/Escherichia_coli_K-12 Escherichia coli K-12]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3QDR OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3QDR FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=TRS:2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL'>TRS</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.65Å</td></tr> |
| - | <tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MLY:N-DIMETHYL-LYSINE'>MLY</scene></td></tr> | + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MLY:N-DIMETHYL-LYSINE'>MLY</scene>, <scene name='pdbligand=TRS:2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL'>TRS</scene></td></tr> |
| - | <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[3qdp|3qdp]]</td></tr>
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3qdr FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3qdr OCA], [https://pdbe.org/3qdr PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3qdr RCSB], [https://www.ebi.ac.uk/pdbsum/3qdr PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3qdr ProSAT]</span></td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">tolA, cim, excC, lky, b0739, JW0729 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 Escherichia coli]), caa, ColA ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=546 Citrobacter freundii])</td></tr>
| + | |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3qdr FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3qdr OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3qdr RCSB], [http://www.ebi.ac.uk/pdbsum/3qdr PDBsum]</span></td></tr> | + | |
| | </table> | | </table> |
| | == Function == | | == Function == |
| - | [[http://www.uniprot.org/uniprot/TOLA_ECOLI TOLA_ECOLI]] Involved in the TonB-independent uptake of group A colicins (colicins A, E1, E2, E3, and K). Necessary for the colicins to reach their respective targets after initial binding to the bacteria. Also involved in the translocation of bacteriophage DNA. | + | [https://www.uniprot.org/uniprot/TOLA_ECOLI TOLA_ECOLI] Involved in the TonB-independent uptake of group A colicins (colicins A, E1, E2, E3, and K). Necessary for the colicins to reach their respective targets after initial binding to the bacteria. Also involved in the translocation of bacteriophage DNA. |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
| Line 19: |
Line 18: |
| | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> |
| | </div> | | </div> |
| | + | <div class="pdbe-citations 3qdr" style="background-color:#fffaf0;"></div> |
| | | | |
| | ==See Also== | | ==See Also== |
| - | *[[Colicin|Colicin]] | + | *[[Colicin 3D structures|Colicin 3D structures]] |
| | *[[TolA|TolA]] | | *[[TolA|TolA]] |
| | == References == | | == References == |
| Line 28: |
Line 28: |
| | </StructureSection> | | </StructureSection> |
| | [[Category: Citrobacter freundii]] | | [[Category: Citrobacter freundii]] |
| - | [[Category: Escherichia coli]] | + | [[Category: Escherichia coli K-12]] |
| - | [[Category: Li, C]]
| + | [[Category: Large Structures]] |
| - | [[Category: Cola]]
| + | [[Category: Li C]] |
| - | [[Category: Cola binds to tola]]
| + | |
| - | [[Category: Complex]]
| + | |
| - | [[Category: Methylation]]
| + | |
| - | [[Category: Protein transport-toxin complex]]
| + | |
| - | [[Category: Tola]] | + | |
| - | [[Category: Translocation]] | + | |
| Structural highlights
Function
TOLA_ECOLI Involved in the TonB-independent uptake of group A colicins (colicins A, E1, E2, E3, and K). Necessary for the colicins to reach their respective targets after initial binding to the bacteria. Also involved in the translocation of bacteriophage DNA.
Publication Abstract from PubMed
The Tol assembly of proteins is an interacting network of proteins located in the Eschericha coli cell envelope that transduces energy and contributes to cell integrity. TolA is central to this network linking the inner and outer membranes by interactions with TolQ, TolR, TolB and Pal. Group A colicins, such as ColA, parasitize the Tol network through interactions with TolA and/or TolB, to facilitate translocation through the cell envelope to reach their cytotoxic site of action. We have determined the first structure of the C-terminal domain of TolA (TolAIII) bound to an N-terminal ColA polypeptide (TA53-107). The interface region of the TA53-107-TolAIII complex consists of polar contacts linking residues R92 to R96 of ColA with residues L375-P380 of TolA which constitutes a beta-strand addition commonly seen in more promiscuous protein-protein contacts. The interface region also includes three cation-pi interactions (Y58-K368, Y90-K379, F94-K396) which have not been observed in any other colicin-Tol protein complex. Mutagenesis of the interface residues of ColA or TolA revealed that the effect on the interaction was cumulative; single mutations of either partner had no effect on ColA activity, whereas mutations of three or more residues significantly reduced ColA activity. Mutagenesis of the aromatic ring component of the cation-pi interacting residues showed Y58 of ColA to be essential for the stability of complex formation. TA53-107 binds on the opposite side of TolAIII to that used by g3p, ColN or TolB, illustrating the flexible nature of TolA as a periplasmic hub protein.
Structural evidence that colicin A binds to a novel binding site of TolA in the E.coli periplasm.,Li C, Zhang Y, Vankemmelbeke M, Hecht O, Aleanizy FS, Macdonald C, Moore GR, James R, Penfold CN J Biol Chem. 2012 Apr 9. PMID:22493500[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
See Also
References
- ↑ Li C, Zhang Y, Vankemmelbeke M, Hecht O, Aleanizy FS, Macdonald C, Moore GR, James R, Penfold CN. Structural evidence that colicin A binds to a novel binding site of TolA in the E.coli periplasm. J Biol Chem. 2012 Apr 9. PMID:22493500 doi:10.1074/jbc.M112.342246
|
