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1w50

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[[Image:1w50.gif|left|200px]]<br />
 
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<applet load="1w50" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1w50, resolution 1.75&Aring;" />
 
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'''APO STRUCTURE OF BACE (BETA SECRETASE)'''<br />
 
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==Overview==
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==Apo Structure of BACE (Beta Secretase)==
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Human BACE, also known as beta-secretase, shows promise as a potential, therapeutic target for Alzheimer's disease. We determined the apo, structure of BACE to 1.75 A, and a structure of a hydroxyethylamine, inhibitor complex derived by soaking. These show significant active-site, movements compared to previously described BACE structures. Additionally, the structures reveal two pockets that could be targeted by, structure-based drug design.
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<StructureSection load='1w50' size='340' side='right'caption='[[1w50]], [[Resolution|resolution]] 1.75&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[1w50]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1W50 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1W50 FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.75&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=IOD:IODIDE+ION'>IOD</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1w50 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1w50 OCA], [https://pdbe.org/1w50 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1w50 RCSB], [https://www.ebi.ac.uk/pdbsum/1w50 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1w50 ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/BACE1_HUMAN BACE1_HUMAN] Responsible for the proteolytic processing of the amyloid precursor protein (APP). Cleaves at the N-terminus of the A-beta peptide sequence, between residues 671 and 672 of APP, leads to the generation and extracellular release of beta-cleaved soluble APP, and a corresponding cell-associated C-terminal fragment which is later released by gamma-secretase.<ref>PMID:10677483</ref> <ref>PMID:20354142</ref>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/w5/1w50_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1w50 ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Human BACE, also known as beta-secretase, shows promise as a potential therapeutic target for Alzheimer's disease. We determined the apo structure of BACE to 1.75 A, and a structure of a hydroxyethylamine inhibitor complex derived by soaking. These show significant active-site movements compared to previously described BACE structures. Additionally, the structures reveal two pockets that could be targeted by structure-based drug design.
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==About this Structure==
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Apo and inhibitor complex structures of BACE (beta-secretase).,Patel S, Vuillard L, Cleasby A, Murray CW, Yon J J Mol Biol. 2004 Oct 15;343(2):407-16. PMID:15451669<ref>PMID:15451669</ref>
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1W50 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] with IOD as [http://en.wikipedia.org/wiki/ligand ligand]. Active as [http://en.wikipedia.org/wiki/Memapsin_2 Memapsin 2], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.23.46 3.4.23.46] Structure known Active Site: AC2. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1W50 OCA].
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==Reference==
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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Apo and inhibitor complex structures of BACE (beta-secretase)., Patel S, Vuillard L, Cleasby A, Murray CW, Yon J, J Mol Biol. 2004 Oct 15;343(2):407-16. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=15451669 15451669]
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</div>
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[[Category: Homo sapiens]]
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<div class="pdbe-citations 1w50" style="background-color:#fffaf0;"></div>
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[[Category: Memapsin 2]]
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[[Category: Single protein]]
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[[Category: Cleasby, A.]]
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[[Category: Murray, C.W.]]
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[[Category: Patel, S.]]
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[[Category: Vuillard, L.]]
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[[Category: Yon, J.]]
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[[Category: IOD]]
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[[Category: alternative splicing]]
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[[Category: alzheimer's disease]]
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[[Category: aspartic protease]]
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[[Category: aspartyl protease]]
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[[Category: base]]
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[[Category: beta-secretase]]
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[[Category: glycoprotein]]
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[[Category: hydrolase]]
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[[Category: memapsin 2]]
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[[Category: signal]]
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[[Category: transmembrane]]
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[[Category: zymogen]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Mon Nov 5 13:29:39 2007''
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==See Also==
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*[[Beta secretase|Beta secretase]]
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*[[Beta secretase 3D structures|Beta secretase 3D structures]]
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== References ==
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<references/>
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__TOC__
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</StructureSection>
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[[Category: Homo sapiens]]
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[[Category: Large Structures]]
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[[Category: Cleasby A]]
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[[Category: Murray CW]]
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[[Category: Patel S]]
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[[Category: Vuillard L]]
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[[Category: Yon J]]

Current revision

Apo Structure of BACE (Beta Secretase)

PDB ID 1w50

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