4kp4

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==Deciphering cis-trans directionality and visualizing autophosphorylation in histidine kinases.==
==Deciphering cis-trans directionality and visualizing autophosphorylation in histidine kinases.==
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<StructureSection load='4kp4' size='340' side='right' caption='[[4kp4]], [[Resolution|resolution]] 3.00&Aring;' scene=''>
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<StructureSection load='4kp4' size='340' side='right'caption='[[4kp4]], [[Resolution|resolution]] 3.00&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
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<table><tr><td colspan='2'>[[4kp4]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Ecoli Ecoli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4KP4 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4KP4 FirstGlance]. <br>
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<table><tr><td colspan='2'>[[4kp4]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli_K-12 Escherichia coli K-12] and [https://en.wikipedia.org/wiki/Thermotoga_maritima_MSB8 Thermotoga maritima MSB8]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4KP4 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4KP4 FirstGlance]. <br>
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</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ANP:PHOSPHOAMINOPHOSPHONIC+ACID-ADENYLATE+ESTER'>ANP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=PEG:DI(HYDROXYETHYL)ETHER'>PEG</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3&#8491;</td></tr>
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<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">envZ, ThemaDRAFT_0449, TM_0853, b3404, JW3367, ompB, perA, tpo ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=83333 ECOLI])</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ANP:PHOSPHOAMINOPHOSPHONIC+ACID-ADENYLATE+ESTER'>ANP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=PEG:DI(HYDROXYETHYL)ETHER'>PEG</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
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<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Histidine_kinase Histidine kinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.13.3 2.7.13.3] </span></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4kp4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4kp4 OCA], [https://pdbe.org/4kp4 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4kp4 RCSB], [https://www.ebi.ac.uk/pdbsum/4kp4 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4kp4 ProSAT]</span></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4kp4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4kp4 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4kp4 RCSB], [http://www.ebi.ac.uk/pdbsum/4kp4 PDBsum]</span></td></tr>
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</table>
</table>
== Function ==
== Function ==
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[[http://www.uniprot.org/uniprot/ENVZ_ECOLI ENVZ_ECOLI]] Member of the two-component regulatory system EnvZ/OmpR involved in the regulation of osmoregulation (genes ompF and ompC). EnvZ functions as a membrane-associated protein kinase that phosphorylates OmpR in response to environmental signals.
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[https://www.uniprot.org/uniprot/Q9WZV7_THEMA Q9WZV7_THEMA] [https://www.uniprot.org/uniprot/ENVZ_ECOLI ENVZ_ECOLI] Member of the two-component regulatory system EnvZ/OmpR involved in the regulation of osmoregulation (genes ompF and ompC). EnvZ functions as a membrane-associated protein kinase that phosphorylates OmpR in response to environmental signals.
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Reversible protein phosphorylation is the most widespread regulatory mechanism in signal transduction. Autophosphorylation in a dimeric sensor histidine kinase is the first step in two-component signalling, the predominant signal-transduction device in bacteria. Despite being the most abundant sensor kinases in nature, the molecular bases of the histidine kinase autophosphorylation mechanism are still unknown. Furthermore, it has been demonstrated that autophosphorylation can occur in two directions, cis (intrasubunit) or trans (intersubunit) within the dimeric histidine kinase. Here, we present the crystal structure of the complete catalytic machinery of a chimeric histidine kinase. The structure shows an asymmetric histidine kinase dimer where one subunit is caught performing the autophosphorylation reaction. A structure-guided functional analysis on HK853 and EnvZ, two prototypical cis- and trans-phosphorylating histidine kinases, has allowed us to decipher the catalytic mechanism of histidine kinase autophosphorylation, which seems to be common independently of the reaction directionality.
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Visualizing autophosphorylation in histidine kinases.,Casino P, Miguel-Romero L, Marina A Nat Commun. 2014 Feb 6;5:3258. doi: 10.1038/ncomms4258. PMID:24500224<ref>PMID:24500224</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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== References ==
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<references/>
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__TOC__
__TOC__
</StructureSection>
</StructureSection>
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[[Category: Ecoli]]
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[[Category: Escherichia coli K-12]]
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[[Category: Histidine kinase]]
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[[Category: Large Structures]]
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[[Category: Casino, P]]
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[[Category: Thermotoga maritima MSB8]]
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[[Category: Marina, A]]
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[[Category: Casino P]]
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[[Category: Miguel-Romero, L]]
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[[Category: Marina A]]
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[[Category: Atp binding]]
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[[Category: Miguel-Romero L]]
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[[Category: Bergerat fold]]
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[[Category: Four helix-bundle]]
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[[Category: Kinase and phosphotransferase]]
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[[Category: Transferase-signaling protein complex]]
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Current revision

Deciphering cis-trans directionality and visualizing autophosphorylation in histidine kinases.

PDB ID 4kp4

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