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1tcm

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[[Image:1tcm.gif|left|200px]]<br />
 
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<applet load="1tcm" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1tcm, resolution 2.2&Aring;" />
 
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'''CYCLODEXTRIN GLYCOSYLTRANSFERASE W616A MUTANT FROM BACILLUS CIRCULANS STRAIN 251'''<br />
 
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==Overview==
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==CYCLODEXTRIN GLYCOSYLTRANSFERASE W616A MUTANT FROM BACILLUS CIRCULANS STRAIN 251==
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The E-domain of cyclodextrin glycosyltransferase (CGTase) (EC 2.4.1.19), from Bacillus circulans strain 251 is a putative raw starch binding, domain. Analysis of the maltose-dependent CGTase crystal structure, revealed that each enzyme molecule contained three maltose molecules, situated at contact points between protein molecules. Two of these, maltoses were bound to specific sites in the E-domain, the third maltose, was bound at the C-domain. To delineate the roles in raw starch binding, and cyclization reaction kinetics of the two maltose binding sites in the, E-domain, we replaced Trp-616 and Trp-662 of maltose binding site 1 and, Tyr-633 of maltose binding site 2 by alanines using site-directed, mutagenesis. Purified mutant CGTases were characterized with respect to, raw starch binding and cyclization reaction kinetics on both soluble and, raw starch. The results show that maltose binding site 1 is most important, for raw starch binding, whereas maltose binding site 2 is involved in, guiding linear starch chains into the active site. beta-Cyclodextrin, causes product inhibition by interfering with catalysis in the active site, and the function of maltose binding site 2 in the E-domain. CGTase mutants, in the E-domain maltose binding site 1 could no longer be crystallized as, maltose-dependent monomers. Instead, the W616A mutant CGTase protein was, successfully crystallized as a carbohydrate-independent dimer; its, structure has been refined to 2.2 A resolution. The three-dimensional, structure shows that, within the error limits, neither the absence of, carbohydrates nor the W616A mutation caused significant further, conformational changes. The modified starch binding and cyclization, kinetic properties observed with the mutant CGTase proteins thus can be, directly related to the amino acid replacements.
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<StructureSection load='1tcm' size='340' side='right'caption='[[1tcm]], [[Resolution|resolution]] 2.20&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[1tcm]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Niallia_circulans Niallia circulans]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1TCM OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1TCM FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.2&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1tcm FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1tcm OCA], [https://pdbe.org/1tcm PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1tcm RCSB], [https://www.ebi.ac.uk/pdbsum/1tcm PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1tcm ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/CDGT2_NIACI CDGT2_NIACI]
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/tc/1tcm_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1tcm ConSurf].
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<div style="clear:both"></div>
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==About this Structure==
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==See Also==
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1TCM is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Bacillus_circulans Bacillus circulans] with CA as [http://en.wikipedia.org/wiki/ligand ligand]. Active as [http://en.wikipedia.org/wiki/Cyclomaltodextrin_glucanotransferase Cyclomaltodextrin glucanotransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.4.1.19 2.4.1.19] Structure known Active Sites: CA1, CA2, CA3, CA4, CT1, CT2, MB1, MB2, MB3, MB4, MB5 and MB6. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1TCM OCA].
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*[[Glycosyltransferase 3D structures|Glycosyltransferase 3D structures]]
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__TOC__
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==Reference==
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</StructureSection>
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The raw starch binding domain of cyclodextrin glycosyltransferase from Bacillus circulans strain 251., Penninga D, van der Veen BA, Knegtel RM, van Hijum SA, Rozeboom HJ, Kalk KH, Dijkstra BW, Dijkhuizen L, J Biol Chem. 1996 Dec 20;271(51):32777-84. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=8955113 8955113]
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[[Category: Large Structures]]
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[[Category: Bacillus circulans]]
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[[Category: Niallia circulans]]
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[[Category: Cyclomaltodextrin glucanotransferase]]
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[[Category: Dijkstra BW]]
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[[Category: Single protein]]
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[[Category: Knegtel RMA]]
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[[Category: Dijkstra, B.W.]]
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[[Category: Knegtel, R.M.A.]]
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[[Category: CA]]
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[[Category: calcium]]
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[[Category: glycosyltransferase]]
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[[Category: signal]]
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[[Category: transferase]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Mon Nov 5 13:30:12 2007''
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Current revision

CYCLODEXTRIN GLYCOSYLTRANSFERASE W616A MUTANT FROM BACILLUS CIRCULANS STRAIN 251

PDB ID 1tcm

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