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| - | [[Image:2czr.gif|left|200px]] | |
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| - | {{Structure
| + | ==Crystal structure of TBP-interacting protein (Tk-TIP26) and implications for its inhibition mechanism of the interaction between TBP and TATA-DNA== |
| - | |PDB= 2czr |SIZE=350|CAPTION= <scene name='initialview01'>2czr</scene>, resolution 2.30Å
| + | <StructureSection load='2czr' size='340' side='right'caption='[[2czr]], [[Resolution|resolution]] 2.30Å' scene=''> |
| - | |SITE=
| + | == Structural highlights == |
| - | |LIGAND= <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene> and <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>
| + | <table><tr><td colspan='2'>[[2czr]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Thermococcus_kodakarensis_KOD1 Thermococcus kodakarensis KOD1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2CZR OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2CZR FirstGlance]. <br> |
| - | |ACTIVITY=
| + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.3Å</td></tr> |
| - | |GENE=
| + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr> |
| - | }}
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2czr FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2czr OCA], [https://pdbe.org/2czr PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2czr RCSB], [https://www.ebi.ac.uk/pdbsum/2czr PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2czr ProSAT]</span></td></tr> |
| - | | + | </table> |
| - | '''Crystal structure of TBP-interacting protein (Tk-TIP26) and implications for its inhibition mechanism of the interaction between TBP and TATA-DNA'''
| + | == Function == |
| - | | + | [https://www.uniprot.org/uniprot/Q9V2W6_THEKO Q9V2W6_THEKO] |
| - | | + | __TOC__ |
| - | ==Overview== | + | </StructureSection> |
| - | TATA-binding protein (TBP)-interacting protein from the hyperthermophilic archaeon Thermococcus kodakaraensis strain KOD1 (Tk-TIP26) is a possible transcription regulatory protein in Thermococcales. Here, we report the crystal structure of Tk-TIP26 determined at 2.3 A resolution with multiple-wavelength anomalous dispersion (MAD) method. The overall structure of Tk-TIP26 consists of two domains. The N-terminal domain forms an alpha/beta structure, in which three alpha-helices enclose the central beta-sheet. The topology of this domain is similar to that of holliday junction resolvase Hjc from Pyrococcus furiosus. The C-terminal domain comprises three alpha-helices, six beta-strands, and two 3(10)-helices. In the dimer structure of Tk-TIP26, two molecules are related with the crystallographic twofold axis, and these molecules rigidly interact with each other via hydrogen bonds. The complex of Tk-TIP26/Tk-TBP is isolated and analyzed by SDS-PAGE and gel filtration column chromatography, resulting in a stoichiometric ratio of the interaction between Tk-TIP26 and Tk-TBP of 4:2, i.e., two dimer molecules of Tk-TIP26 formed a complex with one dimeric TBP. The electrostatic surfaces of Tk-TIP26 and TBP from Pyrocuccus woesei (PwTBP) allowed us to build a model of the Tk-TIP26/TBP complex, and to propose the inhibition mechanism where two dimer molecules of Tk-TIP26 bind to a dimeric TBP, preventing its binding to TATA-DNA.
| + | [[Category: Large Structures]] |
| - | | + | [[Category: Thermococcus kodakarensis KOD1]] |
| - | ==About this Structure== | + | [[Category: Inoue T]] |
| - | 2CZR is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Thermococcus_kodakarensis Thermococcus kodakarensis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2CZR OCA].
| + | [[Category: Kai Y]] |
| - | | + | [[Category: Kanaya S]] |
| - | ==Reference== | + | [[Category: Matsuda T]] |
| - | Crystal structure of TBP-interacting protein (Tk-TIP26) and implications for its inhibition mechanism of the interaction between TBP and TATA-DNA., Yamamoto T, Matsuda T, Inoue T, Matsumura H, Morikawa M, Kanaya S, Kai Y, Protein Sci. 2006 Jan;15(1):152-61. Epub 2005 Dec 1. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/16322571 16322571]
| + | [[Category: Matsumura H]] |
| - | [[Category: Single protein]] | + | [[Category: Morikawa M]] |
| - | [[Category: Thermococcus kodakarensis]]
| + | [[Category: Yamamoto T]] |
| - | [[Category: Inoue, T.]] | + | |
| - | [[Category: Kai, Y.]]
| + | |
| - | [[Category: Kanaya, S.]] | + | |
| - | [[Category: Matsuda, T.]] | + | |
| - | [[Category: Matsumura, H.]] | + | |
| - | [[Category: Morikawa, M.]] | + | |
| - | [[Category: Yamamoto, T.]] | + | |
| - | [[Category: GOL]] | + | |
| - | [[Category: ZN]] | + | |
| - | [[Category: hyperthermophilic archaeon]] | + | |
| - | [[Category: tata-binding protein (tbp)]] | + | |
| - | [[Category: tbp-interacting protein (tip)]] | + | |
| - | [[Category: zn-finger motif]] | + | |
| - | | + | |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 16:21:36 2008''
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