2d7e

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[[Image:2d7e.jpg|left|200px]]
 
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{{Structure
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==Crystal structure of N-terminal domain of PriA from E.coli==
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|PDB= 2d7e |SIZE=350|CAPTION= <scene name='initialview01'>2d7e</scene>, resolution 2.5&Aring;
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<StructureSection load='2d7e' size='340' side='right'caption='[[2d7e]], [[Resolution|resolution]] 2.50&Aring;' scene=''>
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|SITE=
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== Structural highlights ==
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|LIGAND=
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<table><tr><td colspan='2'>[[2d7e]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2D7E OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2D7E FirstGlance]. <br>
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|ACTIVITY=
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.5&#8491;</td></tr>
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|GENE= priA ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 Escherichia coli])
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2d7e FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2d7e OCA], [https://pdbe.org/2d7e PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2d7e RCSB], [https://www.ebi.ac.uk/pdbsum/2d7e PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2d7e ProSAT]</span></td></tr>
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}}
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/PRIA_ECOLI PRIA_ECOLI] Involved in the restart of stalled replication forks. Recognizes and binds the arrested nascent DNA chain at stalled replication forks. It can open the DNA duplex, via its helicase activity, and promote assembly of the primosome and loading of the major replicative helicase DnaB onto DNA. Is also involved in initiation of normal DNA replication in various plasmids and phages. Binds to branched DNA structures that resemble D-loops or to the primosome assembly site (PAS). Binds to DNA in two distinct modes, either dependent on or independent of the 3' terminus recognition.<ref>PMID:2825188</ref> <ref>PMID:8366072</ref> <ref>PMID:10356325</ref> <ref>PMID:10956036</ref> <ref>PMID:11929519</ref> <ref>PMID:12622722</ref> <ref>PMID:12917421</ref> <ref>PMID:15576682</ref> <ref>PMID:17483094</ref> <ref>PMID:23264623</ref>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/d7/2d7e_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2d7e ConSurf].
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<div style="clear:both"></div>
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'''Crystal structure of N-terminal domain of PriA from E.coli'''
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==See Also==
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*[[Helicase 3D structures|Helicase 3D structures]]
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== References ==
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==Overview==
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<references/>
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PriA, a DEXH-type DNA helicase, binds specifically to the 3' end of DNA through its N-terminal domain, and is a candidate sensor protein that recognizes arrested DNA replication forks in bacteria. We crystallized an N-terminal fragment of PriA in the absence and the presence of oligonucleotides to elucidate the structural basis for the specific recognition of the 3' terminus of DNA.
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__TOC__
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</StructureSection>
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==About this Structure==
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2D7E is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2D7E OCA].
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==Reference==
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Crystallization and preliminary crystallographic analysis of the N-terminal domain of PriA from Escherichia coli., Sasaki K, Ose T, Tanaka T, Mizukoshi T, Ishigaki T, Maenaka K, Masai H, Kohda D, Biochim Biophys Acta. 2006 Jan;1764(1):157-60. Epub 2005 Oct 3. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/16226927 16226927]
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[[Category: Escherichia coli]]
[[Category: Escherichia coli]]
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[[Category: Single protein]]
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[[Category: Large Structures]]
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[[Category: Kohda, D.]]
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[[Category: Kohda D]]
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[[Category: Maenaka, K.]]
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[[Category: Maenaka K]]
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[[Category: Masai, H.]]
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[[Category: Masai H]]
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[[Category: Ose, T.]]
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[[Category: Ose T]]
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[[Category: Sasaki, K.]]
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[[Category: Sasaki K]]
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[[Category: inter-twined]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 16:23:57 2008''
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Current revision

Crystal structure of N-terminal domain of PriA from E.coli

PDB ID 2d7e

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