4m8n

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==Crystal Structure of PlexinC1/Rap1B Complex==
==Crystal Structure of PlexinC1/Rap1B Complex==
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<StructureSection load='4m8n' size='340' side='right' caption='[[4m8n]], [[Resolution|resolution]] 3.29&Aring;' scene=''>
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<StructureSection load='4m8n' size='340' side='right'caption='[[4m8n]], [[Resolution|resolution]] 3.29&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
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<table><tr><td colspan='2'>[[4m8n]] is a 8 chain structure with sequence from [http://en.wikipedia.org/wiki/Brachidanio_rerio Brachidanio rerio] and [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4M8N OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4M8N FirstGlance]. <br>
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<table><tr><td colspan='2'>[[4m8n]] is a 8 chain structure with sequence from [https://en.wikipedia.org/wiki/Danio_rerio Danio rerio] and [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4M8N OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4M8N FirstGlance]. <br>
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</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=AF3:ALUMINUM+FLUORIDE'>AF3</scene>, <scene name='pdbligand=GDP:GUANOSINE-5-DIPHOSPHATE'>GDP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3.294&#8491;</td></tr>
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<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">Plexin C1, plxnc1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=7955 Brachidanio rerio]), OK/SW-cl.11, Rap 1B, RAP1B ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=AF3:ALUMINUM+FLUORIDE'>AF3</scene>, <scene name='pdbligand=GDP:GUANOSINE-5-DIPHOSPHATE'>GDP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4m8n FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4m8n OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4m8n RCSB], [http://www.ebi.ac.uk/pdbsum/4m8n PDBsum]</span></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4m8n FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4m8n OCA], [https://pdbe.org/4m8n PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4m8n RCSB], [https://www.ebi.ac.uk/pdbsum/4m8n PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4m8n ProSAT]</span></td></tr>
</table>
</table>
== Function ==
== Function ==
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[[http://www.uniprot.org/uniprot/RAP1B_HUMAN RAP1B_HUMAN]] GTP-binding protein that possesses intrinsic GTPase activity. Contributes to the polarizing activity of KRIT1 and CDH5 in the establishment and maintenance of correct endothelial cell polarity and vascular lumen. Required for the localization of phosphorylated PRKCZ, PARD3 and TIAM1 to the cell junction.<ref>PMID:20332120</ref> <ref>PMID:18660803</ref>
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[https://www.uniprot.org/uniprot/Q5RGW1_DANRE Q5RGW1_DANRE]
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Plexins are cell surface receptors that bind semaphorins and transduce signals for regulating neuronal axon guidance and other processes. Plexin signaling depends on their cytoplasmic GTPase activating protein (GAP) domain, which specifically inactivates the Ras homolog Rap through an ill-defined non-canonical catalytic mechanism. The plexin GAP is activated by semaphorin-induced dimerization, the structural basis for which remained unknown. Here we present the crystal structures of the active dimer of zebrafish PlexinC1 cytoplasmic region in the apo state and in complex with Rap. The structures show that the dimerization induces a large-scale conformational change in plexin, which opens the GAP active site to allow Rap binding. Plexin stabilizes the switch II region of Rap in an unprecedented conformation, bringing Gln63 in Rap into the active site for catalyzing GTP hydrolysis. The structures also explain the unique Rap-specificity of plexins. Mutational analyses support that these mechanisms underlie plexin activation and signaling. DOI:http://dx.doi.org/10.7554/eLife.01279.001.
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Structural basis for activation and non-canonical catalysis of the Rap GTPase activating protein domain of plexin.,Wang Y, Pascoe HG, Brautigam CA, He H, Zhang X Elife. 2013 Oct 1;2:e01279. doi: 10.7554/eLife.01279. PMID:24137545<ref>PMID:24137545</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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==See Also==
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</div>
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*[[GTP-binding protein 3D structures|GTP-binding protein 3D structures]]
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== References ==
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*[[Plexin 3D structures|Plexin 3D structures]]
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<references/>
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__TOC__
__TOC__
</StructureSection>
</StructureSection>
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[[Category: Brachidanio rerio]]
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[[Category: Danio rerio]]
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[[Category: Human]]
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[[Category: Homo sapiens]]
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[[Category: Brautigam, C A]]
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[[Category: Large Structures]]
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[[Category: He, H]]
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[[Category: Brautigam CA]]
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[[Category: Pascoe, H G]]
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[[Category: He H]]
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[[Category: Wang, Y]]
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[[Category: Pascoe HG]]
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[[Category: Zhang, X]]
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[[Category: Wang Y]]
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[[Category: Gtp binding]]
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[[Category: Zhang X]]
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[[Category: Gtpase]]
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[[Category: Gtpase activating protein]]
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[[Category: Magnesium binding]]
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[[Category: Membrane]]
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[[Category: Rap]]
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[[Category: Signaling protein]]
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Current revision

Crystal Structure of PlexinC1/Rap1B Complex

PDB ID 4m8n

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