4bk7

From Proteopedia

(Difference between revisions)

Current revision

Crystal Structure of a variant of the Major Birch Pollen Allergen Bet v 1

Structural highlights

4bk7 is a 1 chain structure with sequence from Betula pendula. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:	X-ray diffraction, Resolution 1.14Å
Ligands:	, ,
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

BEV1A_BETPN May be a general steroid carrier protein (By similarity).

Publication Abstract from PubMed

Many allergens share several biophysical characteristics, including the capability to undergo oligomerisation. The dimerization mechanism in Bet v 1 and its allergenic properties are so far poorly understood. Here, we report crystal structures of dimeric Bet v 1, revealing a non-canonical incorporation of cysteine at position 5 instead of genetically encoded tyrosine. Cysteine polysulfide bridging stabilized different dimeric assemblies, depending on the polysulfide linker length. These dimers represent quaternary arrangements that are frequently observed in related proteins, reflecting their prevalence in unmodified Bet v 1. These conclusions were corroborated by characteristic immunologic properties of monomeric and dimeric allergen variants. Hereby, residue 5 could be identified as an allergenic hot spot in Bet v 1. The presented results refine fundamental principles in protein chemistry, and emphasize the importance of protein modifications in understanding the molecular basis of allergenicity.

Stabilization of the dimeric birch pollen allergen Bet v 1 impacts its immunological properties.,Kofler S, Ackaert C, Samonig M, Asam C, Briza P, Horejs-Hoeck J, Cabrele C, Ferreira F, Duschl A, Huber C, Brandstetter H J Biol Chem. 2013 Nov 19. PMID:24253036^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Kofler S, Ackaert C, Samonig M, Asam C, Briza P, Horejs-Hoeck J, Cabrele C, Ferreira F, Duschl A, Huber C, Brandstetter H. Stabilization of the dimeric birch pollen allergen Bet v 1 impacts its immunological properties. J Biol Chem. 2013 Nov 19. PMID:24253036 doi:http://dx.doi.org/10.1074/jbc.M113.518795

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/4bk7"

Categories: Betula pendula | Large Structures | Brandstetter H | Kofler S

@@ Line 1: / Line 1: @@
 ==Crystal Structure of a variant of the Major Birch Pollen Allergen Bet v 1==
-<StructureSection load='4bk7' size='340' side='right' caption='[[4bk7]], [[Resolution|resolution]] 1.14&Aring;' scene=''>
+<StructureSection load='4bk7' size='340' side='right'caption='[[4bk7]], [[Resolution|resolution]] 1.14&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[4bk7]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Betpn Betpn]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4BK7 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4BK7 FirstGlance]. <br>
+<table><tr><td colspan='2'>[[4bk7]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Betula_pendula Betula pendula]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4BK7 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4BK7 FirstGlance]. <br>
-</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=MPD:(4S)-2-METHYL-2,4-PENTANEDIOL'>MPD</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.14&#8491;</td></tr>
-<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[4bk6|4bk6]], [[4bkc|4bkc]], [[4bkd|4bkd]]</td></tr>
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MPD:(4S)-2-METHYL-2,4-PENTANEDIOL'>MPD</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4bk7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4bk7 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4bk7 RCSB], [http://www.ebi.ac.uk/pdbsum/4bk7 PDBsum]</span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4bk7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4bk7 OCA], [https://pdbe.org/4bk7 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4bk7 RCSB], [https://www.ebi.ac.uk/pdbsum/4bk7 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4bk7 ProSAT]</span></td></tr>
 </table>
 == Function ==
-[[http://www.uniprot.org/uniprot/BEV1A_BETPN BEV1A_BETPN]] May be a general steroid carrier protein (By similarity).
+[https://www.uniprot.org/uniprot/BEV1A_BETPN BEV1A_BETPN] May be a general steroid carrier protein (By similarity).
 <div style="background-color:#fffaf0;">
 == Publication Abstract from PubMed ==
@@ Line 17: / Line 18: @@
 From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
 </div>
+<div class="pdbe-citations 4bk7" style="background-color:#fffaf0;"></div>
 == References ==
 <references/>
 __TOC__
 </StructureSection>
-[[Category: Betpn]]
+[[Category: Betula pendula]]
-[[Category: Brandstetter, H]]
+[[Category: Large Structures]]
-[[Category: Kofler, S]]
+[[Category: Brandstetter H]]
-[[Category: Allergen]]
+[[Category: Kofler S]]
-[[Category: Pr-10]]

4bk7

From Proteopedia

Current revision

Crystal Structure of a variant of the Major Birch Pollen Allergen Bet v 1

Structural highlights

Function

Publication Abstract from PubMed

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

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