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| | ==Crystal structure of 2-Fluorohistine labeled Protective Antigen (pH 5.8)== | | ==Crystal structure of 2-Fluorohistine labeled Protective Antigen (pH 5.8)== |
| - | <StructureSection load='3q8f' size='340' side='right' caption='[[3q8f]], [[Resolution|resolution]] 2.10Å' scene=''> | + | <StructureSection load='3q8f' size='340' side='right'caption='[[3q8f]], [[Resolution|resolution]] 2.10Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[3q8f]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Bacillus_anthracis Bacillus anthracis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3Q8F OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3Q8F FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[3q8f]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Bacillus_anthracis Bacillus anthracis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3Q8F OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3Q8F FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=PG4:TETRAETHYLENE+GLYCOL'>PG4</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.1Å</td></tr> |
| - | <tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=2HF:2-FLUORO-L-HISTIDINE'>2HF</scene></td></tr> | + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=2HF:2-FLUORO-L-HISTIDINE'>2HF</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=PG4:TETRAETHYLENE+GLYCOL'>PG4</scene></td></tr> |
| - | <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[3q8a|3q8a]], [[3q8b|3q8b]], [[3q8c|3q8c]], [[3q8e|3q8e]]</td></tr>
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3q8f FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3q8f OCA], [https://pdbe.org/3q8f PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3q8f RCSB], [https://www.ebi.ac.uk/pdbsum/3q8f PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3q8f ProSAT]</span></td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">pagA, pag, pXO1-110, BXA0164, GBAA_pXO1_0164 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1392 Bacillus anthracis])</td></tr>
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| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3q8f FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3q8f OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3q8f RCSB], [http://www.ebi.ac.uk/pdbsum/3q8f PDBsum]</span></td></tr> | + | |
| | </table> | | </table> |
| | == Function == | | == Function == |
| - | [[http://www.uniprot.org/uniprot/PAG_BACAN PAG_BACAN]] One of the three proteins composing the anthrax toxin, the agent which infects many mammalian species and that may cause death. PA binds to a receptor (ATR) in sensitive eukaryotic cells, thereby facilitating the translocation of the enzymatic toxin components, edema factor and lethal factor, across the target cell membrane. PA associated with LF causes death when injected, PA associated with EF produces edema. PA induces immunity to infection with anthrax. | + | [https://www.uniprot.org/uniprot/PAG_BACAN PAG_BACAN] One of the three proteins composing the anthrax toxin, the agent which infects many mammalian species and that may cause death. PA binds to a receptor (ATR) in sensitive eukaryotic cells, thereby facilitating the translocation of the enzymatic toxin components, edema factor and lethal factor, across the target cell membrane. PA associated with LF causes death when injected, PA associated with EF produces edema. PA induces immunity to infection with anthrax. |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> |
| | </div> | | </div> |
| - | | + | <div class="pdbe-citations 3q8f" style="background-color:#fffaf0;"></div> |
| - | ==See Also== | + | |
| - | *[[Anthrax protective antigen|Anthrax protective antigen]]
| + | |
| | == References == | | == References == |
| | <references/> | | <references/> |
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| | </StructureSection> | | </StructureSection> |
| | [[Category: Bacillus anthracis]] | | [[Category: Bacillus anthracis]] |
| - | [[Category: Andra, K K]] | + | [[Category: Large Structures]] |
| - | [[Category: Bann, J G]] | + | [[Category: Andra KK]] |
| - | [[Category: Battaile, K P]] | + | [[Category: Bann JG]] |
| - | [[Category: Janowiak, B E]] | + | [[Category: Battaile KP]] |
| - | [[Category: Lovell, S]] | + | [[Category: Janowiak BE]] |
| - | [[Category: Rajapaksha, M]] | + | [[Category: Lovell S]] |
| - | [[Category: 2-fluorohistidine]]
| + | [[Category: Rajapaksha M]] |
| - | [[Category: Anthrax]]
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| - | [[Category: Ph stability]]
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| - | [[Category: Protective antigen]]
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| - | [[Category: Protein binding]]
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| Structural highlights
Function
PAG_BACAN One of the three proteins composing the anthrax toxin, the agent which infects many mammalian species and that may cause death. PA binds to a receptor (ATR) in sensitive eukaryotic cells, thereby facilitating the translocation of the enzymatic toxin components, edema factor and lethal factor, across the target cell membrane. PA associated with LF causes death when injected, PA associated with EF produces edema. PA induces immunity to infection with anthrax.
Publication Abstract from PubMed
The anthrax protective antigen (PA) binds to the host cellular receptor capillary morphogenesis protein 2 (CMG2) with high affinity. In order to gain a better understanding of how pH may affect binding to the receptor, we have investigated the kinetics of binding as a function of pH to the full-length monomeric PA and to two variants: a 2-fluorohistidine-labeled PA (2-FHisPA), which is approximately 1 pH unit more stable to variations in pH than WT, and an approximately 1 pH unit less stable variant in which Trp346 in the domain 2beta(3) -2beta(4) loop is substituted with a Phe (W346F). We show using stopped-flow fluorescence that the binding rate increases as the pH is lowered for all proteins, with little influence on the rate of dissociation. In addition, we have crystallized PA and the two variants and examine the influence of pH on structure. In contrast to previous X-ray studies, the domain 2beta(3) -2beta(4) loop undergoes little change in structure from pH approximately 8 to pH 5.5 for the WT protein, but for the 2-FHis labeled and W346F mutant there are changes in structure consistent with previous X-ray studies. In accord with pH stability studies, we find that the average B-factor values increase by approximately 20-30% for all three proteins at low pH. Our results suggest that for the full-length PA, low pH increases the binding affinity, likely through a change in structure that favors a more "bound-like" conformation.
pH effects on binding between the anthrax protective antigen and the host cellular receptor CMG2.,Rajapaksha M, Lovell S, Janowiak BE, Andra KK, Battaile KP, Bann JG Protein Sci. 2012 Jul 31. doi: 10.1002/pro.2136. PMID:22855243[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Rajapaksha M, Lovell S, Janowiak BE, Andra KK, Battaile KP, Bann JG. pH effects on binding between the anthrax protective antigen and the host cellular receptor CMG2. Protein Sci. 2012 Jul 31. doi: 10.1002/pro.2136. PMID:22855243 doi:10.1002/pro.2136
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