4j7e

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
Current revision (12:01, 1 March 2024) (edit) (undo)
 
(2 intermediate revisions not shown.)
Line 1: Line 1:
 +
==The 1.63A crystal structure of humanized Xenopus MDM2 with a nutlin fragment, RO5524529==
==The 1.63A crystal structure of humanized Xenopus MDM2 with a nutlin fragment, RO5524529==
-
<StructureSection load='4j7e' size='340' side='right' caption='[[4j7e]], [[Resolution|resolution]] 1.63&Aring;' scene=''>
+
<StructureSection load='4j7e' size='340' side='right'caption='[[4j7e]], [[Resolution|resolution]] 1.63&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
-
<table><tr><td colspan='2'>[[4j7e]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/African_clawed_frog African clawed frog]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4J7E OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4J7E FirstGlance]. <br>
+
<table><tr><td colspan='2'>[[4j7e]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Xenopus_laevis Xenopus laevis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4J7E OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4J7E FirstGlance]. <br>
-
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=I29:[(4S,5R)-4,5-BIS(4-CHLOROPHENYL)-2,4,5-TRIMETHYL-4,5-DIHYDRO-1H-IMIDAZOL-1-YL]{4-[3-(METHYLSULFONYL)PROPYL]PIPERAZIN-1-YL}METHANONE'>I29</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
+
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.63&#8491;</td></tr>
-
<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[4j74|4j74]], [[4j7d|4j7d]], [[4ipf|4ipf]], [[4j3e|4j3e]]</td></tr>
+
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=I29:[(4S,5R)-4,5-BIS(4-CHLOROPHENYL)-2,4,5-TRIMETHYL-4,5-DIHYDRO-1H-IMIDAZOL-1-YL]{4-[3-(METHYLSULFONYL)PROPYL]PIPERAZIN-1-YL}METHANONE'>I29</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
-
<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">mdm2 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=8355 African clawed frog])</td></tr>
+
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4j7e FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4j7e OCA], [https://pdbe.org/4j7e PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4j7e RCSB], [https://www.ebi.ac.uk/pdbsum/4j7e PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4j7e ProSAT]</span></td></tr>
-
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4j7e FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4j7e OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4j7e RCSB], [http://www.ebi.ac.uk/pdbsum/4j7e PDBsum]</span></td></tr>
+
</table>
</table>
== Function ==
== Function ==
-
[[http://www.uniprot.org/uniprot/MDM2_XENLA MDM2_XENLA]] E3 ubiquitin-protein ligase that mediates ubiquitination of p53/TP53, leading to its degration by the proteasome (By similarity).
+
[https://www.uniprot.org/uniprot/MDM2_XENLA MDM2_XENLA] E3 ubiquitin-protein ligase that mediates ubiquitination of p53/TP53, leading to its degration by the proteasome (By similarity).
-
<div style="background-color:#fffaf0;">
+
-
== Publication Abstract from PubMed ==
+
-
Protein-protein interaction (PPI) systems represent a rich potential source of targets for drug discovery, but historically have proven to be difficult, particularly in the lead identification stage. Application of the fragment-based approach may help toward success with this target class. To provide an example toward understanding the potential issues associated with such an application, we have deconstructed one of the best established protein-protein inhibitors, the Nutlin series that inhibits the interaction between MDM2 and p53, into fragments, and surveyed the resulting binding properties using heteronuclear single quantum coherence nuclear magnetic resonance (HSQC NMR), surface plasmon resonance (SPR), and X-ray crystallography. We report the relative contributions toward binding affinity for each of the key substituents of the Nutlin molecule and show that this series could hypothetically have been discovered via a fragment approach. We find that the smallest fragment of Nutlin that retains binding accesses two subpockets of MDM2 and has a molecular weight at the high end of the range that normally defines fragments.
+
-
 
+
-
Deconstruction of a nutlin: dissecting the binding determinants of a potent protein-protein interaction inhibitor.,Fry DC, Wartchow C, Graves B, Janson C, Lukacs C, Kammlott U, Belunis C, Palme S, Klein C, Vu B ACS Med Chem Lett. 2013 May 24;4(7):660-5. doi: 10.1021/ml400062c. eCollection, 2013 Jul 11. PMID:24900726<ref>PMID:24900726</ref>
+
-
 
+
-
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
+
-
</div>
+
==See Also==
==See Also==
-
*[[MDM2|MDM2]]
+
*[[MDM2 3D structures|MDM2 3D structures]]
-
== References ==
+
-
<references/>
+
__TOC__
__TOC__
</StructureSection>
</StructureSection>
-
[[Category: African clawed frog]]
+
[[Category: Large Structures]]
-
[[Category: Graves, B]]
+
[[Category: Xenopus laevis]]
-
[[Category: Janson, C]]
+
[[Category: Graves B]]
-
[[Category: Lukacs, C]]
+
[[Category: Janson C]]
-
[[Category: E3 ubiquitin ligase]]
+
[[Category: Lukacs C]]
-
[[Category: Imidazoline]]
+
-
[[Category: Ligase-antagonist complex]]
+
-
[[Category: Mdm2]]
+
-
[[Category: Nucleus]]
+
-
[[Category: P53]]
+
-
[[Category: Protein-protein interaction]]
+

Current revision

The 1.63A crystal structure of humanized Xenopus MDM2 with a nutlin fragment, RO5524529

PDB ID 4j7e

Drag the structure with the mouse to rotate

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/4j7e"
Personal tools