3qui

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==Crystal structure of Pseudomonas aeruginosa Hfq in complex with ADPNP==
==Crystal structure of Pseudomonas aeruginosa Hfq in complex with ADPNP==
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<StructureSection load='3qui' size='340' side='right' caption='[[3qui]], [[Resolution|resolution]] 1.93&Aring;' scene=''>
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<StructureSection load='3qui' size='340' side='right'caption='[[3qui]], [[Resolution|resolution]] 1.93&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
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<table><tr><td colspan='2'>[[3qui]] is a 6 chain structure with sequence from [http://en.wikipedia.org/wiki/Pseudomonas_aeruginosa Pseudomonas aeruginosa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3QUI OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3QUI FirstGlance]. <br>
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<table><tr><td colspan='2'>[[3qui]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Pseudomonas_aeruginosa Pseudomonas aeruginosa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3QUI OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3QUI FirstGlance]. <br>
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</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ADE:ADENINE'>ADE</scene>, <scene name='pdbligand=ADP:ADENOSINE-5-DIPHOSPHATE'>ADP</scene>, <scene name='pdbligand=ANP:PHOSPHOAMINOPHOSPHONIC+ACID-ADENYLATE+ESTER'>ANP</scene>, <scene name='pdbligand=PEG:DI(HYDROXYETHYL)ETHER'>PEG</scene></td></tr>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.933&#8491;</td></tr>
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<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[1u1t|1u1t]], [[1u1s|1u1s]], [[3m4g|3m4g]], [[3inz|3inz]], [[1hk9|1hk9]], [[3gib|3gib]]</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ADE:ADENINE'>ADE</scene>, <scene name='pdbligand=ADP:ADENOSINE-5-DIPHOSPHATE'>ADP</scene>, <scene name='pdbligand=ANP:PHOSPHOAMINOPHOSPHONIC+ACID-ADENYLATE+ESTER'>ANP</scene>, <scene name='pdbligand=PEG:DI(HYDROXYETHYL)ETHER'>PEG</scene></td></tr>
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<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">hfq, PA4944 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=287 Pseudomonas aeruginosa])</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3qui FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3qui OCA], [https://pdbe.org/3qui PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3qui RCSB], [https://www.ebi.ac.uk/pdbsum/3qui PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3qui ProSAT]</span></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3qui FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3qui OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3qui RCSB], [http://www.ebi.ac.uk/pdbsum/3qui PDBsum]</span></td></tr>
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</table>
</table>
== Function ==
== Function ==
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[[http://www.uniprot.org/uniprot/HFQ_PSEAE HFQ_PSEAE]] RNA chaperone that binds small regulatory RNA (sRNAs) and mRNAs to facilitate mRNA translational regulation in response to envelope stress, environmental stress and changes in metabolite concentrations. Also binds with high specificity to tRNAs (By similarity).
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[https://www.uniprot.org/uniprot/HFQ_PSEAE HFQ_PSEAE] RNA chaperone that binds small regulatory RNA (sRNAs) and mRNAs to facilitate mRNA translational regulation in response to envelope stress, environmental stress and changes in metabolite concentrations. Also binds with high specificity to tRNAs (By similarity).
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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The Hfq protein forms a doughnut-shaped homohexamer that possesses RNA-binding activity. There are two distinct RNA-binding surfaces located on the proximal and the distal sides of the hexamer. The proximal side is involved in the binding of mRNA and small noncoding RNAs (sRNAs), while the distal side has an affinity for A-rich RNA sequences. In this work, the ability of various ribonucleotides to form complexes with Hfq from Pseudomonas aeruginosa has been tested using X-ray crystallography. ATP and ADPNP have been located in the distal R-site, which is a site for poly(A) RNA binding. UTP has been found in the so-called lateral RNA-binding site at the proximal surface. CTP has been found in both the distal R-site and the proximal U-binding site. GTP did not form a complex with Hfq under the conditions tested. The results have demonstrated the power of the crystallographic method for locating ribonucleotides and predicting single-stranded RNA-binding sites on the protein surface.
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Hfq binds ribonucleotides in three different RNA-binding sites.,Murina V, Lekontseva N, Nikulin A Acta Crystallogr D Biol Crystallogr. 2013 Aug;69(Pt 8):1504-13. doi:, 10.1107/S090744491301010X. Epub 2013 Jul 18. PMID:23897473<ref>PMID:23897473</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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==See Also==
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</div>
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*[[Protein Hfq 3D structures|Protein Hfq 3D structures]]
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== References ==
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<references/>
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__TOC__
__TOC__
</StructureSection>
</StructureSection>
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[[Category: Large Structures]]
[[Category: Pseudomonas aeruginosa]]
[[Category: Pseudomonas aeruginosa]]
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[[Category: Gabdulkhakov, A G]]
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[[Category: Gabdulkhakov AG]]
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[[Category: Murina, V N]]
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[[Category: Murina VN]]
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[[Category: Nikulin, A D]]
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[[Category: Nikulin AD]]
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[[Category: Lsm]]
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[[Category: Mrna]]
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[[Category: Rna binding protein]]
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[[Category: Rna chaperone]]
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[[Category: Rna-binding protein]]
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[[Category: Srna]]
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Current revision

Crystal structure of Pseudomonas aeruginosa Hfq in complex with ADPNP

PDB ID 3qui

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