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2e3r

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[[Image:2e3r.jpg|left|200px]]
 
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{{Structure
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==Crystal structure of CERT START domain in complex with C18-ceramide (P1)==
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|PDB= 2e3r |SIZE=350|CAPTION= <scene name='initialview01'>2e3r</scene>, resolution 1.65&Aring;
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<StructureSection load='2e3r' size='340' side='right'caption='[[2e3r]], [[Resolution|resolution]] 1.65&Aring;' scene=''>
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|SITE= <scene name='pdbsite=AC1:18c+Binding+Site+For+Residue+A+1100'>AC1</scene> and <scene name='pdbsite=AC2:18c+Binding+Site+For+Residue+B+1200'>AC2</scene>
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== Structural highlights ==
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|LIGAND= <scene name='pdbligand=18C:'>18C</scene>
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<table><tr><td colspan='2'>[[2e3r]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2E3R OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2E3R FirstGlance]. <br>
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|ACTIVITY=
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.65&#8491;</td></tr>
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|GENE= CERT ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 Homo sapiens])
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=18C:N-((E,2S,3R)-1,3-DIHYDROXYOCTADEC-4-EN-2-YL)STEARAMIDE'>18C</scene></td></tr>
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}}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2e3r FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2e3r OCA], [https://pdbe.org/2e3r PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2e3r RCSB], [https://www.ebi.ac.uk/pdbsum/2e3r PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2e3r ProSAT]</span></td></tr>
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</table>
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'''Crystal structure of CERT START domain in complex with C18-ceramide (P1)'''
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== Disease ==
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[https://www.uniprot.org/uniprot/CERT_HUMAN CERT_HUMAN] The disease is caused by variants affecting the gene represented in this entry.
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== Function ==
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==Overview==
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[https://www.uniprot.org/uniprot/CERT_HUMAN CERT_HUMAN] Shelters ceramides and diacylglycerol lipids inside its START domain and mediates the intracellular trafficking of ceramides and diacylglycerol lipids in a non-vesicular manner.<ref>PMID:14685229</ref> <ref>PMID:17591919</ref> <ref>PMID:18184806</ref> <ref>PMID:20036255</ref>
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In mammalian cells, ceramide is synthesized in the endoplasmic reticulum and transferred to the Golgi apparatus for conversion to sphingomyelin. Ceramide transport occurs in a nonvesicular manner and is mediated by CERT, a cytosolic 68-kDa protein with a C-terminal steroidogenic acute regulatory protein-related lipid transfer (START) domain. The CERT START domain efficiently transfers natural D-erythro-C16-ceramide, but not lipids with longer (C20) amide-acyl chains. The molecular mechanisms of ceramide specificity, both stereo-specific recognition and length limit, are not well understood. Here we report the crystal structures of the CERT START domain in its apo-form and in complex with ceramides having different acyl chain lengths. In these complex structures, one ceramide molecule is buried in a long amphiphilic cavity. At the far end of the cavity, the amide and hydroxyl groups of ceramide form a hydrogen bond network with specific amino acid residues that play key roles in stereo-specific ceramide recognition. At the head of the ceramide molecule, there is no extra space to accommodate additional bulky groups. The two aliphatic chains of ceramide are surrounded by the hydrophobic wall of the cavity, whose size and shape dictate the length limit for cognate ceramides. Furthermore, local high-crystallographic B-factors suggest that the alpha-3 and the Omega1 loop might work as a gate to incorporate the ceramide into the cavity. Thus, the structures demonstrate the structural basis for the mechanism by which CERT can distinguish ceramide from other lipid types yet still recognize multiple species of ceramides.
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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==About this Structure==
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Check<jmol>
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2E3R is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2E3R OCA].
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/e3/2e3r_consurf.spt"</scriptWhenChecked>
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==Reference==
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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Structural basis for specific lipid recognition by CERT responsible for nonvesicular trafficking of ceramide., Kudo N, Kumagai K, Tomishige N, Yamaji T, Wakatsuki S, Nishijima M, Hanada K, Kato R, Proc Natl Acad Sci U S A. 2008 Jan 15;105(2):488-93. Epub 2008 Jan 9. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/18184806 18184806]
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2e3r ConSurf].
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<div style="clear:both"></div>
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== References ==
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<references/>
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__TOC__
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</StructureSection>
[[Category: Homo sapiens]]
[[Category: Homo sapiens]]
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[[Category: Single protein]]
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[[Category: Large Structures]]
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[[Category: Hanada, K.]]
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[[Category: Hanada K]]
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[[Category: Kato, R.]]
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[[Category: Kato R]]
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[[Category: Kudo, N.]]
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[[Category: Kudo N]]
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[[Category: Kumagai, K.]]
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[[Category: Kumagai K]]
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[[Category: Nishijima, M.]]
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[[Category: Nishijima M]]
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[[Category: Wakatsuki, S.]]
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[[Category: Wakatsuki S]]
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[[Category: 18C]]
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[[Category: ceramide transfer]]
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[[Category: cert]]
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[[Category: lipid transfer protein]]
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[[Category: lipid transport]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 16:34:59 2008''
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Current revision

Crystal structure of CERT START domain in complex with C18-ceramide (P1)

PDB ID 2e3r

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