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2e3w

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[[Image:2e3w.gif|left|200px]]
 
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{{Structure
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==X-ray structure of native RNase A==
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|PDB= 2e3w |SIZE=350|CAPTION= <scene name='initialview01'>2e3w</scene>, resolution 1.05&Aring;
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<StructureSection load='2e3w' size='340' side='right'caption='[[2e3w]], [[Resolution|resolution]] 1.05&Aring;' scene=''>
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|SITE=
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== Structural highlights ==
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|LIGAND= <scene name='pdbligand=CL:CHLORIDE ION'>CL</scene>
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<table><tr><td colspan='2'>[[2e3w]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Bos_taurus Bos taurus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2E3W OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2E3W FirstGlance]. <br>
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|ACTIVITY= [http://en.wikipedia.org/wiki/Pancreatic_ribonuclease Pancreatic ribonuclease], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.27.5 3.1.27.5]
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.05&#8491;</td></tr>
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|GENE=
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene></td></tr>
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}}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2e3w FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2e3w OCA], [https://pdbe.org/2e3w PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2e3w RCSB], [https://www.ebi.ac.uk/pdbsum/2e3w PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2e3w ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/RNAS1_BOVIN RNAS1_BOVIN] Endonuclease that catalyzes the cleavage of RNA on the 3' side of pyrimidine nucleotides. Acts on single stranded and double stranded RNA.<ref>PMID:7479688</ref>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/e3/2e3w_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2e3w ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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The total chemical synthesis of RNase A using modern chemical ligation methods is described, illustrating the significant advances that have been made in chemical protein synthesis since Gutte and Merrifield's pioneering preparation of RNase A in 1969. The identity of the synthetic product was confirmed through rigorous characterization, including the determination of the X-ray crystal structure to 1.1 Angstrom resolution.
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'''X-ray structure of native RNase A'''
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Total synthesis by modern chemical ligation methods and high resolution (1.1 A) X-ray structure of ribonuclease A.,Boerema DJ, Tereshko VA, Kent SB Biopolymers. 2008;90(3):278-86. PMID:17610259<ref>PMID:17610259</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 2e3w" style="background-color:#fffaf0;"></div>
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==About this Structure==
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==See Also==
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2E3W is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Bos_taurus Bos taurus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2E3W OCA].
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*[[Ribonuclease 3D structures|Ribonuclease 3D structures]]
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== References ==
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==Reference==
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<references/>
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Total synthesis by modern chemical ligation methods &amp; high resolution (1.1Angstrom) X-ray structure of ribonuclease A., Boerema DJ, Tereshko VA, Kent SB, Biopolymers. 2007 Jul 3;. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/17610259 17610259]
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__TOC__
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</StructureSection>
[[Category: Bos taurus]]
[[Category: Bos taurus]]
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[[Category: Pancreatic ribonuclease]]
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[[Category: Large Structures]]
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[[Category: Single protein]]
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[[Category: Boerema DJ]]
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[[Category: Boerema, D J.]]
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[[Category: Kent SBH]]
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[[Category: Kent, S B.H.]]
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[[Category: Tereshko VA]]
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[[Category: Tereshko, V A.]]
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[[Category: CL]]
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[[Category: alpha-helix]]
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[[Category: beta-sheet]]
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[[Category: hydrolase]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 16:35:03 2008''
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Current revision

X-ray structure of native RNase A

PDB ID 2e3w

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