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1bhm

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==RESTRICTION ENDONUCLEASE BAMHI COMPLEX WITH DNA==
==RESTRICTION ENDONUCLEASE BAMHI COMPLEX WITH DNA==
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<StructureSection load='1bhm' size='340' side='right' caption='[[1bhm]], [[Resolution|resolution]] 2.20&Aring;' scene=''>
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<StructureSection load='1bhm' size='340' side='right'caption='[[1bhm]], [[Resolution|resolution]] 2.20&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
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<table><tr><td colspan='2'>[[1bhm]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Bacillus_amyloliquefaciens Bacillus amyloliquefaciens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1BHM OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1BHM FirstGlance]. <br>
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<table><tr><td colspan='2'>[[1bhm]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Bacillus_amyloliquefaciens Bacillus amyloliquefaciens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1BHM OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1BHM FirstGlance]. <br>
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</td></tr><tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Type_II_site-specific_deoxyribonuclease Type II site-specific deoxyribonuclease], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.21.4 3.1.21.4] </span></td></tr>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.2&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1bhm FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1bhm OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1bhm RCSB], [http://www.ebi.ac.uk/pdbsum/1bhm PDBsum]</span></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1bhm FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1bhm OCA], [https://pdbe.org/1bhm PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1bhm RCSB], [https://www.ebi.ac.uk/pdbsum/1bhm PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1bhm ProSAT]</span></td></tr>
</table>
</table>
== Function ==
== Function ==
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[[http://www.uniprot.org/uniprot/T2BA_BACAM T2BA_BACAM]] Recognizes the double-stranded sequence GGATCC and cleaves after G-1.
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[https://www.uniprot.org/uniprot/T2BA_BACAM T2BA_BACAM] Recognizes the double-stranded sequence GGATCC and cleaves after G-1.
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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The crystal structure of restriction endonuclease Bam HI complexed to DNA has been determined at 2.2 angstrom resolution. The DNA binds in the cleft and retains a B-DNA type of conformation. The enzyme, however, undergoes a series of conformational changes, including rotation of subunits and folding of disordered regions. The most striking conformational change is the unraveling of carboxyl-terminal alpha helices to form partially disordered "arms." The arm from one subunit fits into the minor groove while the arm from the symmetry related subunit follows the DNA sugar-phosphate backbone. Recognition of DNA base pairs occurs primarily in the major groove, with a few interactions occurring in the minor groove. Tightly bound water molecules play an equally important role as side chain and main chain atoms in the recognition of base pairs. The complex also provides new insights into the mechanism by which the enzyme catalyzes the hydrolysis of DNA phosphodiester groups.
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Structure of Bam HI endonuclease bound to DNA: partial folding and unfolding on DNA binding.,Newman M, Strzelecka T, Dorner LF, Schildkraut I, Aggarwal AK Science. 1995 Aug 4;269(5224):656-63. PMID:7624794<ref>PMID:7624794</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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==See Also==
==See Also==
*[[BamHI|BamHI]]
*[[BamHI|BamHI]]
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*[[Endonuclease|Endonuclease]]
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*[[Endonuclease 3D structures|Endonuclease 3D structures]]
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== References ==
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<references/>
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__TOC__
__TOC__
</StructureSection>
</StructureSection>
[[Category: Bacillus amyloliquefaciens]]
[[Category: Bacillus amyloliquefaciens]]
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[[Category: Type II site-specific deoxyribonuclease]]
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[[Category: Large Structures]]
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[[Category: Aggarwal, A K]]
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[[Category: Aggarwal AK]]
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[[Category: Newman, M]]
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[[Category: Newman M]]
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[[Category: Hydrolase]]
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[[Category: Hydrolase-dna complex]]
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[[Category: Nuclease]]
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Current revision

RESTRICTION ENDONUCLEASE BAMHI COMPLEX WITH DNA

PDB ID 1bhm

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