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2egb

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[[Image:2egb.jpg|left|200px]]
 
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{{Structure
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==Crystal structure of Glu140 to Asn mutant of Diphthine synthase==
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|PDB= 2egb |SIZE=350|CAPTION= <scene name='initialview01'>2egb</scene>, resolution 1.90&Aring;
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<StructureSection load='2egb' size='340' side='right'caption='[[2egb]], [[Resolution|resolution]] 1.90&Aring;' scene=''>
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|SITE=
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== Structural highlights ==
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|LIGAND= <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>, <scene name='pdbligand=SAH:S-ADENOSYL-L-HOMOCYSTEINE'>SAH</scene>, <scene name='pdbligand=MES:2-(N-MORPHOLINO)-ETHANESULFONIC+ACID'>MES</scene> and <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>
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<table><tr><td colspan='2'>[[2egb]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Pyrococcus_horikoshii_OT3 Pyrococcus horikoshii OT3]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2EGB OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2EGB FirstGlance]. <br>
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|ACTIVITY= [http://en.wikipedia.org/wiki/Diphthine_synthase Diphthine synthase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.1.1.98 2.1.1.98]
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.9&#8491;</td></tr>
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|GENE= dphB ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=53953 Pyrococcus horikoshii])
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=MES:2-(N-MORPHOLINO)-ETHANESULFONIC+ACID'>MES</scene>, <scene name='pdbligand=SAH:S-ADENOSYL-L-HOMOCYSTEINE'>SAH</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
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}}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2egb FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2egb OCA], [https://pdbe.org/2egb PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2egb RCSB], [https://www.ebi.ac.uk/pdbsum/2egb PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2egb ProSAT], [https://www.topsan.org/Proteins/RSGI/2egb TOPSAN]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/DPHB_PYRHO DPHB_PYRHO] S-adenosyl-L-methionine-dependent methyltransferase that catalyzes the trimethylation of the amino group of the modified target histidine residue in translation elongation factor 2 (EF-2), to form an intermediate called diphthine. The three successive methylation reactions represent the second step of diphthamide biosynthesis.<ref>PMID:20873788</ref>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/eg/2egb_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2egb ConSurf].
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<div style="clear:both"></div>
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'''Crystal structure of Glu140 to Asn mutant of Diphthine synthase'''
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==See Also==
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*[[Diphthine synthase|Diphthine synthase]]
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== References ==
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==About this Structure==
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<references/>
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2EGB is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Pyrococcus_horikoshii Pyrococcus horikoshii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2EGB OCA].
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__TOC__
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[[Category: Diphthine synthase]]
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</StructureSection>
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[[Category: Pyrococcus horikoshii]]
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[[Category: Large Structures]]
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[[Category: Single protein]]
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[[Category: Pyrococcus horikoshii OT3]]
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[[Category: Kunishima, N.]]
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[[Category: Krishna Swamy BS]]
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[[Category: Matsuura, Y.]]
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[[Category: Kunishima N]]
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[[Category: Mizutani, H.]]
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[[Category: Matsuura Y]]
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[[Category: Murthy, M R.N.]]
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[[Category: Mizutani H]]
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[[Category: RSGI, RIKEN Structural Genomics/Proteomics Initiative.]]
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[[Category: Murthy MRN]]
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[[Category: Simanshu, D K.]]
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[[Category: Simanshu DK]]
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[[Category: Swamy, B S.Krishna.]]
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[[Category: GOL]]
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[[Category: MES]]
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[[Category: SAH]]
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[[Category: SO4]]
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[[Category: national project on protein structural and functional analyse]]
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[[Category: nppsfa]]
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[[Category: riken structural genomics/proteomics initiative]]
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[[Category: rsgi]]
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[[Category: structural genomic]]
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[[Category: transferase]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 16:39:44 2008''
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Current revision

Crystal structure of Glu140 to Asn mutant of Diphthine synthase

PDB ID 2egb

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