This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.

Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.


2ye1

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
Current revision (08:13, 23 August 2023) (edit) (undo)
 
(5 intermediate revisions not shown.)
Line 1: Line 1:
 +
==X-ray structure of the cyan fluorescent proteinmTurquoise-GL (K206A mutant)==
==X-ray structure of the cyan fluorescent proteinmTurquoise-GL (K206A mutant)==
-
<StructureSection load='2ye1' size='340' side='right' caption='[[2ye1]], [[Resolution|resolution]] 1.63&Aring;' scene=''>
+
<StructureSection load='2ye1' size='340' side='right'caption='[[2ye1]], [[Resolution|resolution]] 1.63&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
-
<table><tr><td colspan='2'>[[2ye1]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Aequorea_victoria Aequorea victoria]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2YE1 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2YE1 FirstGlance]. <br>
+
<table><tr><td colspan='2'>[[2ye1]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Aequorea_victoria Aequorea victoria]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2YE1 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2YE1 FirstGlance]. <br>
-
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
+
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.63&#8491;</td></tr>
-
<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=SWG:2-[(4Z)-2-[(1R)-1-AMINO-2-HYDROXY-ETHYL]-4-(1H-INDOL-3-YLMETHYLIDENE)-5-OXO-IMIDAZOL-1-YL]ETHANOIC+ACID'>SWG</scene></td></tr>
+
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=SWG:2-[(4Z)-2-[(1R)-1-AMINO-2-HYDROXY-ETHYL]-4-(1H-INDOL-3-YLMETHYLIDENE)-5-OXO-IMIDAZOL-1-YL]ETHANOIC+ACID'>SWG</scene></td></tr>
-
<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[2emn|2emn]], [[1jc0|1jc0]], [[2fzu|2fzu]], [[1rm9|1rm9]], [[1ema|1ema]], [[1rmo|1rmo]], [[1f09|1f09]], [[1yhg|1yhg]], [[1kp5|1kp5]], [[1q73|1q73]], [[1z1p|1z1p]], [[2b3q|2b3q]], [[1yhh|1yhh]], [[1rmm|1rmm]], [[1emm|1emm]], [[2b3p|2b3p]], [[2fwq|2fwq]], [[1emg|1emg]], [[1rmp|1rmp]], [[1emb|1emb]], [[2emd|2emd]], [[1q4e|1q4e]], [[1q4d|1q4d]], [[1kys|1kys]], [[2wur|2wur]], [[1c4f|1c4f]], [[1q4a|1q4a]], [[1h6r|1h6r]], [[1eml|1eml]], [[1w7t|1w7t]], [[1emk|1emk]], [[2emo|2emo]], [[2ydz|2ydz]], [[2wsn|2wsn]], [[1q4b|1q4b]], [[1w7u|1w7u]], [[1yjf|1yjf]], [[1q4c|1q4c]], [[1yfp|1yfp]], [[1emf|1emf]], [[1yj2|1yj2]], [[2ah8|2ah8]], [[2wso|2wso]], [[1yhi|1yhi]], [[2y0g|2y0g]], [[1eme|1eme]], [[1cv7|1cv7]], [[1gfl|1gfl]], [[1qyq|1qyq]], [[2ye0|2ye0]], [[1jbz|1jbz]], [[1kyr|1kyr]], [[2aha|2aha]], [[1rrx|1rrx]], [[1qxt|1qxt]], [[1myw|1myw]], [[1qy3|1qy3]], [[2yfp|2yfp]], [[1b9c|1b9c]], [[1w7s|1w7s]], [[1jby|1jby]], [[3ztf|3ztf]], [[1f0b|1f0b]], [[1jc1|1jc1]], [[1qyo|1qyo]], [[1bfp|1bfp]], [[1huy|1huy]], [[1hcj|1hcj]], [[1emc|1emc]], [[1z1q|1z1q]], [[1kyp|1kyp]], [[1qyf|1qyf]]</td></tr>
+
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2ye1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2ye1 OCA], [https://pdbe.org/2ye1 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2ye1 RCSB], [https://www.ebi.ac.uk/pdbsum/2ye1 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2ye1 ProSAT]</span></td></tr>
-
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2ye1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2ye1 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2ye1 RCSB], [http://www.ebi.ac.uk/pdbsum/2ye1 PDBsum]</span></td></tr>
+
</table>
</table>
== Function ==
== Function ==
-
[[http://www.uniprot.org/uniprot/GFP_AEQVI GFP_AEQVI]] Energy-transfer acceptor. Its role is to transduce the blue chemiluminescence of the protein aequorin into green fluorescent light by energy transfer. Fluoresces in vivo upon receiving energy from the Ca(2+)-activated photoprotein aequorin.
+
[https://www.uniprot.org/uniprot/GFP_AEQVI GFP_AEQVI] Energy-transfer acceptor. Its role is to transduce the blue chemiluminescence of the protein aequorin into green fluorescent light by energy transfer. Fluoresces in vivo upon receiving energy from the Ca(2+)-activated photoprotein aequorin.
-
<div style="background-color:#fffaf0;">
+
-
== Publication Abstract from PubMed ==
+
-
Optimization of autofluorescent proteins by intensity-based screening of bacteria does not necessarily identify the brightest variant for eukaryotes. We report a strategy to screen excited state lifetimes, which identified cyan fluorescent proteins with long fluorescence lifetimes (&gt;3.7 ns) and high quantum yields (&gt;0.8). One variant, mTurquoise, was 1.5-fold brighter than mCerulean in mammalian cells and decayed mono-exponentially, making it an excellent fluorescence resonance energy transfer (FRET) donor.
+
-
 
+
-
Bright cyan fluorescent protein variants identified by fluorescence lifetime screening.,Goedhart J, van Weeren L, Hink MA, Vischer NO, Jalink K, Gadella TW Jr Nat Methods. 2010 Feb;7(2):137-9. Epub 2010 Jan 17. PMID:20081836<ref>PMID:20081836</ref>
+
-
 
+
-
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
+
-
</div>
+
==See Also==
==See Also==
-
*[[Green Fluorescent Protein|Green Fluorescent Protein]]
+
*[[Green Fluorescent Protein 3D structures|Green Fluorescent Protein 3D structures]]
-
== References ==
+
-
<references/>
+
__TOC__
__TOC__
</StructureSection>
</StructureSection>
[[Category: Aequorea victoria]]
[[Category: Aequorea victoria]]
-
[[Category: Gadella, T W.J]]
+
[[Category: Large Structures]]
-
[[Category: Goedhart, J]]
+
[[Category: Gadella TWJ]]
-
[[Category: Noirclerc-Savoye, M]]
+
[[Category: Goedhart J]]
-
[[Category: Royant, A]]
+
[[Category: Noirclerc-Savoye M]]
-
[[Category: Stetten, D Von]]
+
[[Category: Royant A]]
-
[[Category: Fluorescent protein]]
+
[[Category: Von Stetten D]]
-
[[Category: Fret donor]]
+

Current revision

X-ray structure of the cyan fluorescent proteinmTurquoise-GL (K206A mutant)

PDB ID 2ye1

Drag the structure with the mouse to rotate

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/2ye1"
Personal tools