3syn

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==Crystal structure of FlhF in complex with its activator==
==Crystal structure of FlhF in complex with its activator==
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<StructureSection load='3syn' size='340' side='right' caption='[[3syn]], [[Resolution|resolution]] 3.06&Aring;' scene=''>
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<StructureSection load='3syn' size='340' side='right'caption='[[3syn]], [[Resolution|resolution]] 3.06&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
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<table><tr><td colspan='2'>[[3syn]] is a 8 chain structure with sequence from [http://en.wikipedia.org/wiki/Bacillus_subtilis Bacillus subtilis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3SYN OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3SYN FirstGlance]. <br>
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<table><tr><td colspan='2'>[[3syn]] is a 8 chain structure with sequence from [https://en.wikipedia.org/wiki/Bacillus_subtilis Bacillus subtilis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3SYN OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3SYN FirstGlance]. <br>
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</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=AF3:ALUMINUM+FLUORIDE'>AF3</scene>, <scene name='pdbligand=GDP:GUANOSINE-5-DIPHOSPHATE'>GDP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3.063&#8491;</td></tr>
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<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[2px0|2px0]], [[2px3|2px3]]</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=AF3:ALUMINUM+FLUORIDE'>AF3</scene>, <scene name='pdbligand=GDP:GUANOSINE-5-DIPHOSPHATE'>GDP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
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<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">flhF, BSU16400 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1423 Bacillus subtilis])</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3syn FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3syn OCA], [https://pdbe.org/3syn PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3syn RCSB], [https://www.ebi.ac.uk/pdbsum/3syn PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3syn ProSAT]</span></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3syn FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3syn OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3syn RCSB], [http://www.ebi.ac.uk/pdbsum/3syn PDBsum]</span></td></tr>
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</table>
</table>
== Function ==
== Function ==
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[[http://www.uniprot.org/uniprot/FLHF_BACSU FLHF_BACSU]] Necessary for flagellar biosynthesis. May be involved in translocation of the flagellum. [[http://www.uniprot.org/uniprot/YLXH_BACSU YLXH_BACSU]] Involved in the placement and assembly of flagella (By similarity). Activates the SRP-GTPase activity of FlhF.<ref>PMID:22056770</ref>
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[https://www.uniprot.org/uniprot/FLHF_BACSU FLHF_BACSU] Necessary for flagellar biosynthesis. May be involved in translocation of the flagellum.
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Small G proteins have key roles in signal transduction pathways. They are switched from the signaling 'on' to the non-signaling 'off' state when GTPase-activating proteins (GAPs) provide a catalytic residue. The ancient signal recognition particle (SRP)-type GTPases form GTP-dependent homo- and heterodimers and deviate from the canonical switch paradigm in that no GAPs have been identified. Here we show that the YlxH protein activates the SRP-GTPase FlhF. The crystal structure of the Bacillus subtilis FlhF-effector complex revealed that the effector does not contribute a catalytic residue but positions the catalytic machinery already present in SRP-GTPases. We provide a general concept that might also apply to the RNA-driven activation of the universally conserved, co-translational protein-targeting machinery comprising the SRP-GTPases Ffh and FtsY. Our study exemplifies the evolutionary transition from RNA- to protein-driven activation in SRP-GTPases and suggests that the current view on SRP-mediated protein targeting is incomplete.
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Structural basis for the molecular evolution of SRP-GTPase activation by protein.,Bange G, Kummerer N, Grudnik P, Lindner R, Petzold G, Kressler D, Hurt E, Wild K, Sinning I Nat Struct Mol Biol. 2011 Nov 6;18(12):1376-80. doi: 10.1038/nsmb.2141. PMID:22056770<ref>PMID:22056770</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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==See Also==
==See Also==
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*[[GTP-binding protein|GTP-binding protein]]
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*[[Flagellar biosynthetic protein|Flagellar biosynthetic protein]]
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== References ==
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*[[Flagellar protein 3D structures|Flagellar protein 3D structures]]
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<references/>
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*[[GTP-binding protein 3D structures|GTP-binding protein 3D structures]]
__TOC__
__TOC__
</StructureSection>
</StructureSection>
[[Category: Bacillus subtilis]]
[[Category: Bacillus subtilis]]
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[[Category: Bange, G]]
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[[Category: Large Structures]]
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[[Category: Kuemmerer, N]]
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[[Category: Bange G]]
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[[Category: Sinning, I]]
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[[Category: Kuemmerer N]]
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[[Category: Wild, K]]
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[[Category: Sinning I]]
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[[Category: Biosynthetic protein]]
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[[Category: Wild K]]
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[[Category: Flagellum]]
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[[Category: Gtp-binding protein]]
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[[Category: Gtpase activating protein]]
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[[Category: Protein transport]]
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[[Category: Srp gtpase]]
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[[Category: Type 3 secretion system]]
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Current revision

Crystal structure of FlhF in complex with its activator

PDB ID 3syn

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OCA

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