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4f1e

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==The Crystal Structure of a Human MitoNEET mutant with Asp 67 replaced by a Gly==
==The Crystal Structure of a Human MitoNEET mutant with Asp 67 replaced by a Gly==
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<StructureSection load='4f1e' size='340' side='right' caption='[[4f1e]], [[Resolution|resolution]] 2.40&Aring;' scene=''>
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<StructureSection load='4f1e' size='340' side='right'caption='[[4f1e]], [[Resolution|resolution]] 2.40&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
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<table><tr><td colspan='2'>[[4f1e]] is a 18 chain structure with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4F1E OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4F1E FirstGlance]. <br>
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<table><tr><td colspan='2'>[[4f1e]] is a 18 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4F1E OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4F1E FirstGlance]. <br>
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</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=FES:FE2/S2+(INORGANIC)+CLUSTER'>FES</scene></td></tr>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.4&#8491;</td></tr>
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<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[2qh7|2qh7]], [[3lpq|3lpq]], [[3ew0|3ew0]], [[4f28|4f28]], [[4f2c|4f2c]], [[4ezf|4ezf]]</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=FES:FE2/S2+(INORGANIC)+CLUSTER'>FES</scene></td></tr>
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<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">C10orf70, CISD1, MDS029, ZCD1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 Homo sapiens])</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4f1e FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4f1e OCA], [https://pdbe.org/4f1e PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4f1e RCSB], [https://www.ebi.ac.uk/pdbsum/4f1e PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4f1e ProSAT]</span></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4f1e FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4f1e OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4f1e RCSB], [http://www.ebi.ac.uk/pdbsum/4f1e PDBsum]</span></td></tr>
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</table>
</table>
== Function ==
== Function ==
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[[http://www.uniprot.org/uniprot/CISD1_HUMAN CISD1_HUMAN]] Plays a key role in regulating maximal capacity for electron transport and oxidative phosphorylation (By similarity). May be involved in Fe-S cluster shuttling and/or in redox reactions.<ref>PMID:17584744</ref> <ref>PMID:17766440</ref>
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[https://www.uniprot.org/uniprot/CISD1_HUMAN CISD1_HUMAN] Plays a key role in regulating maximal capacity for electron transport and oxidative phosphorylation (By similarity). May be involved in Fe-S cluster shuttling and/or in redox reactions.<ref>PMID:17584744</ref> <ref>PMID:17766440</ref>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Metalloproteins (MPs) comprise one-third of all known protein structures. This diverse set of proteins contain a plethora of unique inorganic moieties capable of performing chemistry that would otherwise be impossible using only the amino acids found in nature. Most of the well-studied MPs are generally viewed as being very rigid in structure, and it is widely thought that the properties of the metal centers are primarily determined by the small fraction of amino acids that make up the local environment. Here we examine both theoretically and experimentally whether distal regions can influence the metal center in the diabetes drug target mitoNEET. We demonstrate that a loop (L2) 20 A away from the metal center exerts allosteric control over the cluster binding domain and regulates multiple properties of the metal center. Mutagenesis of L2 results in significant shifts in the redox potential of the [2Fe-2S] cluster and orders of magnitude effects on the rate of [2Fe-2S] cluster transfer to an apo-acceptor protein. These surprising effects occur in the absence of any structural changes. An examination of the native basin dynamics of the protein using all-atom simulations shows that twisting in L2 controls scissoring in the cluster binding domain and results in perturbations to one of the cluster-coordinating histidines. These allosteric effects are in agreement with previous folding simulations that predicted L2 could communicate with residues surrounding the metal center. Our findings suggest that long-range dynamical changes in the protein backbone can have a significant effect on the functional properties of MPs.
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Allosteric control in a metalloprotein dramatically alters function.,Baxter EL, Zuris JA, Wang C, Vo PL, Axelrod HL, Cohen AE, Paddock ML, Nechushtai R, Onuchic JN, Jennings PA Proc Natl Acad Sci U S A. 2013 Jan 15;110(3):948-53. doi:, 10.1073/pnas.1208286110. Epub 2012 Dec 27. PMID:23271805<ref>PMID:23271805</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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== References ==
== References ==
<references/>
<references/>
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</StructureSection>
</StructureSection>
[[Category: Homo sapiens]]
[[Category: Homo sapiens]]
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[[Category: Axelrod, H L]]
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[[Category: Large Structures]]
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[[Category: Baxter, E I]]
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[[Category: Axelrod HL]]
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[[Category: Cohen, A E]]
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[[Category: Baxter EI]]
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[[Category: Jennings, P A]]
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[[Category: Cohen AE]]
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[[Category: Nechushtai, R]]
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[[Category: Jennings PA]]
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[[Category: Onuchic, J N]]
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[[Category: Nechushtai R]]
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[[Category: Paddock, M L]]
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[[Category: Onuchic JN]]
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[[Category: Wang, C]]
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[[Category: Paddock ML]]
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[[Category: Zuris, J A]]
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[[Category: Wang C]]
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[[Category: Metal binding protein]]
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[[Category: Zuris JA]]
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[[Category: Mitochondrial outer membrane]]
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[[Category: Zinc finger cdgsh-type domain 1]]
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Current revision

The Crystal Structure of a Human MitoNEET mutant with Asp 67 replaced by a Gly

PDB ID 4f1e

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