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3nh8

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==Crystal structure of murine aminoacylase 3 in complex with N-acetyl-S-1,2-dichlorovinyl-L-cysteine==
==Crystal structure of murine aminoacylase 3 in complex with N-acetyl-S-1,2-dichlorovinyl-L-cysteine==
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<StructureSection load='3nh8' size='340' side='right' caption='[[3nh8]], [[Resolution|resolution]] 2.80&Aring;' scene=''>
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<StructureSection load='3nh8' size='340' side='right'caption='[[3nh8]], [[Resolution|resolution]] 2.80&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
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<table><tr><td colspan='2'>[[3nh8]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3NH8 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3NH8 FirstGlance]. <br>
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<table><tr><td colspan='2'>[[3nh8]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3NH8 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3NH8 FirstGlance]. <br>
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</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=DC2:N-ACETYL-S-[(1S)-1,2-DICHLOROETHYL]-L-CYSTEINE'>DC2</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.802&#8491;</td></tr>
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<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[3nfz|3nfz]], [[3nh4|3nh4]], [[3nh5|3nh5]]</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=DC2:N-ACETYL-S-[(1S)-1,2-DICHLOROETHYL]-L-CYSTEINE'>DC2</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
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<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">ACY-3, Acy3, Aspa2 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10090 Mus musculus])</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3nh8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3nh8 OCA], [https://pdbe.org/3nh8 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3nh8 RCSB], [https://www.ebi.ac.uk/pdbsum/3nh8 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3nh8 ProSAT]</span></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3nh8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3nh8 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3nh8 RCSB], [http://www.ebi.ac.uk/pdbsum/3nh8 PDBsum]</span></td></tr>
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</table>
</table>
== Function ==
== Function ==
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[[http://www.uniprot.org/uniprot/ACY3_MOUSE ACY3_MOUSE]] Plays an important role in deacetylating mercapturic acids in kidney proximal tubules. Also acts on N-acetyl-aromatic amino acids.<ref>PMID:14656720</ref> <ref>PMID:17012540</ref>
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[https://www.uniprot.org/uniprot/ACY3_MOUSE ACY3_MOUSE] Plays an important role in deacetylating mercapturic acids in kidney proximal tubules. Also acts on N-acetyl-aromatic amino acids.<ref>PMID:14656720</ref> <ref>PMID:17012540</ref>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Trichloroethylene (TCE) is one of the most widespread environmental contaminants, which is metabolized to N-acetyl-S-1,2-dichlorovinyl-l-cysteine (NA-DCVC) before being excreted in the urine. Alternatively, NA-DCVC can be deacetylated by aminoacylase 3 (AA3), an enzyme that is highly expressed in the kidney, liver, and brain. NA-DCVC deacetylation initiates the transformation into toxic products that ultimately causes acute renal failure. AA3 inhibition is therefore a target of interest to prevent TCE induced nephrotoxicity. Here we report the crystal structure of recombinant mouse AA3 (mAA3) in the presence of its acetate byproduct and two substrates: N(alpha)-acetyl-l-tyrosine and NA-DCVC. These structures, in conjunction with biochemical data, indicated that AA3 mediates substrate specificity through van der Waals interactions providing a dynamic interaction interface, which facilitates a diverse range of substrates.
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Structures of aminoacylase 3 in complex with acetylated substrates.,Hsieh JM, Tsirulnikov K, Sawaya MR, Magilnick N, Abuladze N, Kurtz I, Abramson J, Pushkin A Proc Natl Acad Sci U S A. 2010 Oct 4. PMID:20921362<ref>PMID:20921362</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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==See Also==
==See Also==
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*[[Aspartoacylase|Aspartoacylase]]
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*[[Aminoacylase 3D structures|Aminoacylase 3D structures]]
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*[[Aspartoacylase 3D structures|Aspartoacylase 3D structures]]
== References ==
== References ==
<references/>
<references/>
__TOC__
__TOC__
</StructureSection>
</StructureSection>
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[[Category: Large Structures]]
[[Category: Mus musculus]]
[[Category: Mus musculus]]
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[[Category: Abramson, J]]
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[[Category: Abramson J]]
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[[Category: Abuladze, N]]
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[[Category: Abuladze N]]
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[[Category: Hsieh, J M]]
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[[Category: Hsieh JM]]
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[[Category: Kurtz, I]]
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[[Category: Kurtz I]]
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[[Category: Magilnick, N]]
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[[Category: Magilnick N]]
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[[Category: Pushkin, A]]
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[[Category: Pushkin A]]
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[[Category: Sawaya, M R]]
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[[Category: Sawaya MR]]
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[[Category: Tsirulnikov, K]]
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[[Category: Tsirulnikov K]]
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[[Category: 2-dichlorovinyl-l-cysteine]]
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[[Category: Hydrolase]]
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[[Category: N-acetyl-s-1]]
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Current revision

Crystal structure of murine aminoacylase 3 in complex with N-acetyl-S-1,2-dichlorovinyl-L-cysteine

PDB ID 3nh8

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