3i59

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==Crystal structure of MtbCRP in complex with N6-cAMP==
==Crystal structure of MtbCRP in complex with N6-cAMP==
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<StructureSection load='3i59' size='340' side='right' caption='[[3i59]], [[Resolution|resolution]] 2.29&Aring;' scene=''>
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<StructureSection load='3i59' size='340' side='right'caption='[[3i59]], [[Resolution|resolution]] 2.29&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
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<table><tr><td colspan='2'>[[3i59]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Mycobacterium_tuberculosis Mycobacterium tuberculosis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3I59 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3I59 FirstGlance]. <br>
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<table><tr><td colspan='2'>[[3i59]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Mycobacterium_tuberculosis Mycobacterium tuberculosis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3I59 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3I59 FirstGlance]. <br>
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</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=N6R:(2R)-N6-(1-METHYL-2-PHENYLETHYL)ADENOSINE-3,5-CYCLIC+MONOPHOSPHATE'>N6R</scene>, <scene name='pdbligand=N6S:(2S)-N6-(1-METHYL-2-PHENYLETHYL)ADENOSINE-3,5-CYCLIC+MONOPHOSPHATE'>N6S</scene></td></tr>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.29&#8491;</td></tr>
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<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene>, <scene name='pdbligand=N6R:(2R)-N6-(1-METHYL-2-PHENYLETHYL)ADENOSINE-3,5-CYCLIC+MONOPHOSPHATE'>N6R</scene>, <scene name='pdbligand=N6S:(2S)-N6-(1-METHYL-2-PHENYLETHYL)ADENOSINE-3,5-CYCLIC+MONOPHOSPHATE'>N6S</scene></td></tr>
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<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[3i54|3i54]]</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3i59 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3i59 OCA], [https://pdbe.org/3i59 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3i59 RCSB], [https://www.ebi.ac.uk/pdbsum/3i59 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3i59 ProSAT]</span></td></tr>
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<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">MT3777, Rv3676 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1773 Mycobacterium tuberculosis])</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3i59 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3i59 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3i59 RCSB], [http://www.ebi.ac.uk/pdbsum/3i59 PDBsum]</span></td></tr>
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</table>
</table>
== Function ==
== Function ==
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[[http://www.uniprot.org/uniprot/O69644_MYCTU O69644_MYCTU]] Global transcriptional regulator that complexes with cAMP and binds to specific DNA promoter sites, causing DNA-bending, to regulate transcription. cAMP improves binding to specific DNA sequences, probably by altering protein conformation. Activates expression of whiB1.<ref>PMID:16946269</ref>
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[https://www.uniprot.org/uniprot/CRPL_MYCTU CRPL_MYCTU] Global transcriptional regulator that complexes with cAMP and binds to specific DNA promoter sites, causing DNA-bending, to regulate transcription. cAMP improves binding to specific DNA sequences, probably by altering protein conformation. The CRP regulon is predicted to contain about 115 genes. Some genes are activated by CRP (rpfA, whiB1) while others are repressed (fadD10). There are 2 CRP-binding sites in the promoter of whiB1, at low concentrations of CRP with or without cAMP transcription of whiB1 is enhanced via site CRP1, then repressed as site CRP2 is filled.<ref>PMID:15882420</ref> <ref>PMID:20028978</ref>
== Evolutionary Conservation ==
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
Check<jmol>
<jmolCheckbox>
<jmolCheckbox>
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<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/i5/3i59_consurf.spt"</scriptWhenChecked>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/i5/3i59_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
<text>to colour the structure by Evolutionary Conservation</text>
<text>to colour the structure by Evolutionary Conservation</text>
</jmolCheckbox>
</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3i59 ConSurf].
<div style="clear:both"></div>
<div style="clear:both"></div>
<div style="background-color:#fffaf0;">
<div style="background-color:#fffaf0;">
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
</div>
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<div class="pdbe-citations 3i59" style="background-color:#fffaf0;"></div>
==See Also==
==See Also==
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*[[Catabolite gene activator protein|Catabolite gene activator protein]]
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*[[Catabolite gene activator protein 3D structures|Catabolite gene activator protein 3D structures]]
== References ==
== References ==
<references/>
<references/>
__TOC__
__TOC__
</StructureSection>
</StructureSection>
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[[Category: Large Structures]]
[[Category: Mycobacterium tuberculosis]]
[[Category: Mycobacterium tuberculosis]]
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[[Category: Bruning, J B]]
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[[Category: Bruning JB]]
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[[Category: Palaninathan, S K]]
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[[Category: Palaninathan SK]]
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[[Category: Reddy, M C]]
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[[Category: Reddy MC]]
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[[Category: Sacchettini, J C]]
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[[Category: Sacchettini JC]]
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[[Category: Smith, D]]
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[[Category: Smith D]]
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[[Category: Structural genomic]]
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[[Category: Thurman C]]
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[[Category: Thurman, C]]
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[[Category: Allosteric mechanism]]
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[[Category: Camp receptor protein]]
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[[Category: Crp]]
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[[Category: Dna binding]]
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[[Category: Dna binding protein]]
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[[Category: Dna-binding]]
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[[Category: Inhibition]]
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[[Category: N6-camp]]
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[[Category: Tbsgc]]
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[[Category: Transcription]]
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[[Category: Transcription regulation]]
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Current revision

Crystal structure of MtbCRP in complex with N6-cAMP

PDB ID 3i59

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