2ewv

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[[Image:2ewv.gif|left|200px]]
 
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{{Structure
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==Crystal Structure of the Pilus Retraction Motor PilT and Bound ADP==
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|PDB= 2ewv |SIZE=350|CAPTION= <scene name='initialview01'>2ewv</scene>, resolution 2.8&Aring;
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<StructureSection load='2ewv' size='340' side='right'caption='[[2ewv]], [[Resolution|resolution]] 2.80&Aring;' scene=''>
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|SITE=
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== Structural highlights ==
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|LIGAND= <scene name='pdbligand=ADP:ADENOSINE-5'-DIPHOSPHATE'>ADP</scene>
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<table><tr><td colspan='2'>[[2ewv]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Aquifex_aeolicus_VF5 Aquifex aeolicus VF5]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2EWV OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2EWV FirstGlance]. <br>
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|ACTIVITY=
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.8&#8491;</td></tr>
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|GENE=
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ADP:ADENOSINE-5-DIPHOSPHATE'>ADP</scene></td></tr>
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}}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2ewv FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2ewv OCA], [https://pdbe.org/2ewv PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2ewv RCSB], [https://www.ebi.ac.uk/pdbsum/2ewv PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2ewv ProSAT]</span></td></tr>
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</table>
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'''Crystal Structure of the Pilus Retraction Motor PilT and Bound ADP'''
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== Function ==
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[https://www.uniprot.org/uniprot/O66950_AQUAE O66950_AQUAE]
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== Evolutionary Conservation ==
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==Overview==
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[[Image:Consurf_key_small.gif|200px|right]]
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PilT is a hexameric ATPase required for bacterial type IV pilus retraction and surface motility. Crystal structures of ADP- and ATP-bound Aquifex aeolicus PilT at 2.8 and 3.2 A resolution show N-terminal PAS-like and C-terminal RecA-like ATPase domains followed by a set of short C-terminal helices. The hexamer is formed by extensive polar subunit interactions between the ATPase core of one monomer and the N-terminal domain of the next. An additional structure captures a nonsymmetric PilT hexamer in which approach of invariant arginines from two subunits to the bound nucleotide forms an enzymatically competent active site. A panel of pilT mutations highlights the importance of the arginines, the PAS-like domain, the polar subunit interface, and the C-terminal helices for retraction. We present a model for ATP binding leading to dramatic PilT domain motions, engagement of the arginine wire, and subunit communication in this hexameric motor. Our conclusions apply to the entire type II/IV secretion ATPase family.
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Check<jmol>
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<jmolCheckbox>
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==About this Structure==
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ew/2ewv_consurf.spt"</scriptWhenChecked>
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2EWV is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Aquifex_aeolicus_vf5 Aquifex aeolicus vf5]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2EWV OCA].
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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==Reference==
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</jmolCheckbox>
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Crystal structures of the pilus retraction motor PilT suggest large domain movements and subunit cooperation drive motility., Satyshur KA, Worzalla GA, Meyer LS, Heiniger EK, Aukema KG, Misic AM, Forest KT, Structure. 2007 Mar;15(3):363-76. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/17355871 17355871]
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2ewv ConSurf].
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[[Category: Aquifex aeolicus vf5]]
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<div style="clear:both"></div>
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[[Category: Single protein]]
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__TOC__
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[[Category: Forest, K T.]]
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</StructureSection>
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[[Category: Satyshur, K A.]]
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[[Category: Aquifex aeolicus VF5]]
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[[Category: ADP]]
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[[Category: Large Structures]]
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[[Category: atpase]]
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[[Category: Forest KT]]
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[[Category: hexameric pilt]]
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[[Category: Satyshur KA]]
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[[Category: pilus retraction motor]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 16:45:27 2008''
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Current revision

Crystal Structure of the Pilus Retraction Motor PilT and Bound ADP

PDB ID 2ewv

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