2f4x
From Proteopedia
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| - | [[Image:2f4x.gif|left|200px]] | ||
| - | + | ==NMR Solution of HIV-1 Lai Kissing Complex== | |
| - | + | <StructureSection load='2f4x' size='340' side='right'caption='[[2f4x]]' scene=''> | |
| - | + | == Structural highlights == | |
| - | + | <table><tr><td colspan='2'>[[2f4x]] is a 2 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2F4X OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2F4X FirstGlance]. <br> | |
| - | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr> | |
| - | | | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2f4x FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2f4x OCA], [https://pdbe.org/2f4x PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2f4x RCSB], [https://www.ebi.ac.uk/pdbsum/2f4x PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2f4x ProSAT]</span></td></tr> |
| - | + | </table> | |
| - | + | <div style="background-color:#fffaf0;"> | |
| - | ''' | + | == Publication Abstract from PubMed == |
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Dimerization of genomic RNA is directly related with the event of encapsidation and maturation of the virion. The initiating sequence of the dimerization is a short autocomplementary region in the hairpin loop SL1. We describe here a new solution structure of the RNA dimerization initiation site (DIS) of HIV-1(Lai). NMR pulsed field-gradient spin-echo techniques and multidimensional heteronuclear NMR spectroscopy indicate that this structure is formed by two hairpins linked by six Watson-Crick GC base pairs. Hinges between the stems and the loops are stabilized by intra and intermolecular interactions involving the A8, A9 and A16 adenines. The coaxial alignment of the three A-type helices present in the structure is supported by previous crystallography analysis but the A8 and A9 adenines are found in a bulged in position. These data suggest the existence of an equilibrium between bulged in and bulged out conformations in solution. | Dimerization of genomic RNA is directly related with the event of encapsidation and maturation of the virion. The initiating sequence of the dimerization is a short autocomplementary region in the hairpin loop SL1. We describe here a new solution structure of the RNA dimerization initiation site (DIS) of HIV-1(Lai). NMR pulsed field-gradient spin-echo techniques and multidimensional heteronuclear NMR spectroscopy indicate that this structure is formed by two hairpins linked by six Watson-Crick GC base pairs. Hinges between the stems and the loops are stabilized by intra and intermolecular interactions involving the A8, A9 and A16 adenines. The coaxial alignment of the three A-type helices present in the structure is supported by previous crystallography analysis but the A8 and A9 adenines are found in a bulged in position. These data suggest the existence of an equilibrium between bulged in and bulged out conformations in solution. | ||
| - | + | A new NMR solution structure of the SL1 HIV-1Lai loop-loop dimer.,Kieken F, Paquet F, Brule F, Paoletti J, Lancelot G Nucleic Acids Res. 2006 Jan 12;34(1):343-52. Print 2006. PMID:16410614<ref>PMID:16410614</ref> | |
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| - | A new NMR solution structure of the SL1 HIV-1Lai loop-loop dimer., Kieken F, Paquet F, Brule F, Paoletti J, Lancelot G | + | |
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| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| + | </div> | ||
| + | <div class="pdbe-citations 2f4x" style="background-color:#fffaf0;"></div> | ||
| + | == References == | ||
| + | <references/> | ||
| + | __TOC__ | ||
| + | </StructureSection> | ||
| + | [[Category: Large Structures]] | ||
| + | [[Category: Brule F]] | ||
| + | [[Category: Kieken F]] | ||
| + | [[Category: Lancelot G]] | ||
| + | [[Category: Paoletti J]] | ||
| + | [[Category: Paquet F]] | ||
Current revision
NMR Solution of HIV-1 Lai Kissing Complex
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