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4gex

From Proteopedia

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Structure of a stabilised ceSAS-6 dimer, second crystal form

Structural highlights

4gex is a 4 chain structure with sequence from Caenorhabditis elegans. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Ligands:
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

SAS6_CAEEL Required for centrosome duplication. Participates in centriole assembly.^[1] ^[2]

Publication Abstract from PubMed

Centrioles are evolutionary conserved organelles that give rise to cilia and flagella as well as centrosomes. Centrioles display a characteristic ninefold symmetry imposed by the spindle assembly abnormal protein 6 (SAS-6) family. SAS-6 from Chlamydomonas reinhardtii and Danio rerio was shown to form ninefold symmetric, ring-shaped oligomers in vitro that were similar to the cartwheels observed in vivo during early steps of centriole assembly in most species. Here, we report crystallographic and EM analyses showing that, instead, Caenorhabotis elegans SAS-6 self-assembles into a spiral arrangement. Remarkably, we find that this spiral arrangement is also consistent with ninefold symmetry, suggesting that two distinct SAS-6 oligomerization architectures can direct the same output symmetry. Sequence analysis suggests that SAS-6 spirals are restricted to specific nematodes. This oligomeric arrangement may provide a structural basis for the presence of a central tube instead of a cartwheel during centriole assembly in these species.

Caenorhabditis elegans centriolar protein SAS-6 forms a spiral that is consistent with imparting a ninefold symmetry.,Hilbert M, Erat MC, Hachet V, Guichard P, Blank ID, Fluckiger I, Slater L, Lowe ED, Hatzopoulos GN, Steinmetz MO, Gonczy P, Vakonakis I Proc Natl Acad Sci U S A. 2013 Jul 9;110(28):11373-8. doi:, 10.1073/pnas.1302721110. Epub 2013 Jun 24. PMID:23798409^[3]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Dammermann A, Muller-Reichert T, Pelletier L, Habermann B, Desai A, Oegema K. Centriole assembly requires both centriolar and pericentriolar material proteins. Dev Cell. 2004 Dec;7(6):815-29. PMID:15572125 doi:S1534580704003806
↑ Leidel S, Delattre M, Cerutti L, Baumer K, Gonczy P. SAS-6 defines a protein family required for centrosome duplication in C. elegans and in human cells. Nat Cell Biol. 2005 Feb;7(2):115-25. PMID:15665853 doi:ncb1220
↑ Hilbert M, Erat MC, Hachet V, Guichard P, Blank ID, Fluckiger I, Slater L, Lowe ED, Hatzopoulos GN, Steinmetz MO, Gonczy P, Vakonakis I. Caenorhabditis elegans centriolar protein SAS-6 forms a spiral that is consistent with imparting a ninefold symmetry. Proc Natl Acad Sci U S A. 2013 Jul 9;110(28):11373-8. doi:, 10.1073/pnas.1302721110. Epub 2013 Jun 24. PMID:23798409 doi:10.1073/pnas.1302721110

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/4gex"

Categories: Caenorhabditis elegans | Large Structures | Erat MC | Vakonakis I

@@ Line 1: / Line 1: @@
 ==Structure of a stabilised ceSAS-6 dimer, second crystal form==
-<StructureSection load='4gex' size='340' side='right' caption='[[4gex]], [[Resolution|resolution]] 2.80&Aring;' scene=''>
+<StructureSection load='4gex' size='340' side='right'caption='[[4gex]], [[Resolution|resolution]] 2.80&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[4gex]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Caenorhabditis_elegans Caenorhabditis elegans]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4GEX OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4GEX FirstGlance]. <br>
+<table><tr><td colspan='2'>[[4gex]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Caenorhabditis_elegans Caenorhabditis elegans]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4GEX OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4GEX FirstGlance]. <br>
-</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
+</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
-<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[2y3v|2y3v]], [[2y3w|2y3w]], [[3pyi|3pyi]], [[3q0y|3q0y]], [[3q0x|3q0x]], [[4g79|4g79]], [[4geu|4geu]], [[4gfa|4gfa]], [[4gfc|4gfc]]</td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4gex FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4gex OCA], [https://pdbe.org/4gex PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4gex RCSB], [https://www.ebi.ac.uk/pdbsum/4gex PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4gex ProSAT]</span></td></tr>
-<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">sas-6, Y45F10D.9 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=6239 Caenorhabditis elegans])</td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4gex FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4gex OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4gex RCSB], [http://www.ebi.ac.uk/pdbsum/4gex PDBsum]</span></td></tr>
 </table>
 == Function ==
-[[http://www.uniprot.org/uniprot/SAS6_CAEEL SAS6_CAEEL]] Required for centrosome duplication. Participates in centriole assembly.<ref>PMID:15572125</ref> <ref>PMID:15665853</ref>
+[https://www.uniprot.org/uniprot/SAS6_CAEEL SAS6_CAEEL] Required for centrosome duplication. Participates in centriole assembly.<ref>PMID:15572125</ref> <ref>PMID:15665853</ref>
 <div style="background-color:#fffaf0;">
 == Publication Abstract from PubMed ==
@@ Line 18: / Line 17: @@
 From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
 </div>
+<div class="pdbe-citations 4gex" style="background-color:#fffaf0;"></div>
 == References ==
 <references/>
@@ Line 23: / Line 23: @@
 </StructureSection>
 [[Category: Caenorhabditis elegans]]
-[[Category: Erat, M C]]
+[[Category: Large Structures]]
-[[Category: Vakonakis, I]]
+[[Category: Erat MC]]
-[[Category: Alpha-beta protein]]
+[[Category: Vakonakis I]]
-[[Category: Beta-sandwich]]
-[[Category: Central tube]]
-[[Category: Centriolar]]
-[[Category: Cytoplasmic]]
-[[Category: Structural protein]]

4gex

From Proteopedia

Current revision

Structure of a stabilised ceSAS-6 dimer, second crystal form

Structural highlights

Function

Publication Abstract from PubMed

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

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