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| | ==Crystal complex of SYS-1/POP-1 at 2.5A resolution== | | ==Crystal complex of SYS-1/POP-1 at 2.5A resolution== |
| - | <StructureSection load='3c2g' size='340' side='right' caption='[[3c2g]], [[Resolution|resolution]] 2.50Å' scene=''> | + | <StructureSection load='3c2g' size='340' side='right'caption='[[3c2g]], [[Resolution|resolution]] 2.50Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[3c2g]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Caenorhabditis_elegans Caenorhabditis elegans]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3C2G OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3C2G FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[3c2g]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Caenorhabditis_elegans Caenorhabditis elegans]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3C2G OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3C2G FirstGlance]. <br> |
| - | </td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.5Å</td></tr> |
| - | <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[3c2h|3c2h]]</td></tr>
| + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">SYS-1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=6239 Caenorhabditis elegans]), POP-1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=6239 Caenorhabditis elegans])</td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3c2g FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3c2g OCA], [https://pdbe.org/3c2g PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3c2g RCSB], [https://www.ebi.ac.uk/pdbsum/3c2g PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3c2g ProSAT]</span></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3c2g FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3c2g OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3c2g RCSB], [http://www.ebi.ac.uk/pdbsum/3c2g PDBsum]</span></td></tr> | + | |
| | </table> | | </table> |
| | == Function == | | == Function == |
| - | [[http://www.uniprot.org/uniprot/POP1_CAEEL POP1_CAEEL]] Part of the Wnt signaling pathway essential for the specification of the mesodermal cell fate in early embryos. Required for asymmetrical division of somatic gonadal precursor descendants which initiate axis formation required to control organ shape. Represses expression of target genes via its interaction with hda-1 histone deacetylase.<ref>PMID:11742996</ref> <ref>PMID:11807036</ref> | + | [https://www.uniprot.org/uniprot/CTNH1_CAEEL CTNH1_CAEEL] Transcription coregulator (PubMed:26073017). Part of the Wnt signaling asymmetry pathway, probably acting downstream of putative frizzled ligand mom-2, Wnt/frizzled receptors lin-17 and mom-5, and dishevelled homolog dsh-2 (PubMed:15020416, PubMed:17296929, PubMed:17567664, PubMed:19386265, PubMed:26073017). Activates or represses target gene expression, depending on upstream Wnt signals and interactions with transcription factors, such as pop-1 (PubMed:26073017, PubMed:35660370). Required for the activation of Wnt-responsive genes in the E blastomere; thereby leading to a role in endoderm specification and gut development (PubMed:17296929, PubMed:17567664). Reciprocal distribution patterns of sys-1 and pop-1/TCF in the daughters of anterior-posterior cell divisions functions in specifying cell fate; a higher sys-1 to pop-1 ratio promotes the posterior cell fate, whereas a low sys-1 to pop-1 ratio promotes the anterior fate (PubMed:17296929, PubMed:17567664). Represses expression of homeobox ttx-3 in neuroblasts of the SIAD/SIBV lineage, perhaps acting by blocking its transcriptional activation by a complex consisting of ref-2 and pop-1 (PubMed:26073017). Required for early organization of the hermaphrodite, but not the male, gonad; involved in generation of regulatory cells, known as the distal tip cells (DTC), and in formation of the somatic gonadal primordium (PubMed:11161562, PubMed:15020416, PubMed:17296929, PubMed:35196020). Involved in regulating asymmetric divisions of the somatic gonadal precursor cells (SGP), Z1 and Z4 (PubMed:15020416).<ref>PMID:11161562</ref> <ref>PMID:15020416</ref> <ref>PMID:17296929</ref> <ref>PMID:17567664</ref> <ref>PMID:19386265</ref> <ref>PMID:26073017</ref> <ref>PMID:35196020</ref> <ref>PMID:35660370</ref> |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> |
| | </div> | | </div> |
| | + | <div class="pdbe-citations 3c2g" style="background-color:#fffaf0;"></div> |
| | == References == | | == References == |
| | <references/> | | <references/> |
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| | </StructureSection> | | </StructureSection> |
| | [[Category: Caenorhabditis elegans]] | | [[Category: Caenorhabditis elegans]] |
| - | [[Category: Amaya, M F]] | + | [[Category: Large Structures]] |
| - | [[Category: Kimble, J]] | + | [[Category: Amaya MF]] |
| - | [[Category: Liu, J]] | + | [[Category: Kimble J]] |
| - | [[Category: Phillips, B T]] | + | [[Category: Liu J]] |
| - | [[Category: Xu, W]] | + | [[Category: Phillips BT]] |
| - | [[Category: Beta-catenin]]
| + | [[Category: Xu W]] |
| - | [[Category: Caenorhabditis elegan]]
| + | |
| - | [[Category: Cell adhesion-transcription complex]]
| + | |
| - | [[Category: Developmental protein]]
| + | |
| - | [[Category: Dna-binding]]
| + | |
| - | [[Category: Nucleus]]
| + | |
| - | [[Category: Phylogeny]]
| + | |
| - | [[Category: Pop-1]]
| + | |
| - | [[Category: Sys-1]]
| + | |
| Structural highlights
Function
CTNH1_CAEEL Transcription coregulator (PubMed:26073017). Part of the Wnt signaling asymmetry pathway, probably acting downstream of putative frizzled ligand mom-2, Wnt/frizzled receptors lin-17 and mom-5, and dishevelled homolog dsh-2 (PubMed:15020416, PubMed:17296929, PubMed:17567664, PubMed:19386265, PubMed:26073017). Activates or represses target gene expression, depending on upstream Wnt signals and interactions with transcription factors, such as pop-1 (PubMed:26073017, PubMed:35660370). Required for the activation of Wnt-responsive genes in the E blastomere; thereby leading to a role in endoderm specification and gut development (PubMed:17296929, PubMed:17567664). Reciprocal distribution patterns of sys-1 and pop-1/TCF in the daughters of anterior-posterior cell divisions functions in specifying cell fate; a higher sys-1 to pop-1 ratio promotes the posterior cell fate, whereas a low sys-1 to pop-1 ratio promotes the anterior fate (PubMed:17296929, PubMed:17567664). Represses expression of homeobox ttx-3 in neuroblasts of the SIAD/SIBV lineage, perhaps acting by blocking its transcriptional activation by a complex consisting of ref-2 and pop-1 (PubMed:26073017). Required for early organization of the hermaphrodite, but not the male, gonad; involved in generation of regulatory cells, known as the distal tip cells (DTC), and in formation of the somatic gonadal primordium (PubMed:11161562, PubMed:15020416, PubMed:17296929, PubMed:35196020). Involved in regulating asymmetric divisions of the somatic gonadal precursor cells (SGP), Z1 and Z4 (PubMed:15020416).[1] [2] [3] [4] [5] [6] [7] [8]
Publication Abstract from PubMed
C. elegans SYS-1 has key functional characteristics of a canonical beta-catenin, but no significant sequence similarity. Here, we report the SYS-1 crystal structure, both on its own and in a complex with POP-1, the C. elegans TCF homolog. The two structures possess signature features of canonical beta-catenin and the beta-catenin/TCF complex that could not be predicted by sequence. Most importantly, SYS-1 bears 12 armadillo repeats and the SYS-1/POP-1 interface is anchored by a conserved salt-bridge, the "charged button." We also modeled structures for three other C. elegans beta-catenins to predict the molecular basis of their distinct binding properties. Finally, we generated a phylogenetic tree, using the region of highest structural similarity between SYS-1 and beta-catenin, and found that SYS-1 clusters robustly within the beta-catenin clade. We conclude that the SYS-1 protein belongs to the beta-catenin family and suggest that additional divergent beta-catenins await discovery.
The C. elegans SYS-1 protein is a bona fide beta-catenin.,Liu J, Phillips BT, Amaya MF, Kimble J, Xu W Dev Cell. 2008 May;14(5):751-61. PMID:18477457[9]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Miskowski J, Li Y, Kimble J. The sys-1 gene and sexual dimorphism during gonadogenesis in Caenorhabditis elegans. Dev Biol. 2001 Feb 1;230(1):61-73. PMID:11161562 doi:10.1006/dbio.2000.9998
- ↑ Siegfried KR, Kidd AR 3rd, Chesney MA, Kimble J. The sys-1 and sys-3 genes cooperate with Wnt signaling to establish the proximal-distal axis of the Caenorhabditis elegans gonad. Genetics. 2004 Jan;166(1):171-86. PMID:15020416 doi:10.1534/genetics.166.1.171
- ↑ Phillips BT, Kidd AR 3rd, King R, Hardin J, Kimble J. Reciprocal asymmetry of SYS-1/beta-catenin and POP-1/TCF controls asymmetric divisions in Caenorhabditis elegans. Proc Natl Acad Sci U S A. 2007 Feb 27;104(9):3231-6. PMID:17296929 doi:10.1073/pnas.0611507104
- ↑ Huang S, Shetty P, Robertson SM, Lin R. Binary cell fate specification during C. elegans embryogenesis driven by reiterated reciprocal asymmetry of TCF POP-1 and its coactivator beta-catenin SYS-1. Development. 2007 Jul;134(14):2685-95. PMID:17567664 doi:10.1242/dev.008268
- ↑ Bertrand V, Hobert O. Linking asymmetric cell division to the terminal differentiation program of postmitotic neurons in C. elegans. Dev Cell. 2009 Apr;16(4):563-75. PMID:19386265 doi:10.1016/j.devcel.2009.02.011
- ↑ Murgan S, Kari W, Rothbächer U, Iché-Torres M, Mélénec P, Hobert O, Bertrand V. Atypical Transcriptional Activation by TCF via a Zic Transcription Factor in C. elegans Neuronal Precursors. Dev Cell. 2015 Jun 22;33(6):737-45. PMID:26073017 doi:10.1016/j.devcel.2015.04.018
- ↑ Thompson JW, Michel MFV, Phillips BT. Centrosomal enrichment and proteasomal degradation of SYS-1/β-catenin requires the microtubule motor dynein. Mol Biol Cell. 2022 May 1;33(5):ar42. PMID:35196020 doi:10.1091/mbc.E22-02-0031
- ↑ Rumley JD, Preston EA, Cook D, Peng FL, Zacharias AL, Wu L, Jileaeva I, Murray JI. pop-1/TCF, ref-2/ZIC and T-box factors regulate the development of anterior cells in the C. elegans embryo. Dev Biol. 2022 Sep;489:34-46. PMID:35660370 doi:10.1016/j.ydbio.2022.05.019
- ↑ Liu J, Phillips BT, Amaya MF, Kimble J, Xu W. The C. elegans SYS-1 protein is a bona fide beta-catenin. Dev Cell. 2008 May;14(5):751-61. PMID:18477457 doi:http://dx.doi.org/S1534-5807(08)00109-3
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