2fbd

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[[Image:2fbd.jpg|left|200px]]
 
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{{Structure
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==The crystallographic structure of the digestive lysozyme 1 from Musca domestica at 1.90 Ang.==
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|PDB= 2fbd |SIZE=350|CAPTION= <scene name='initialview01'>2fbd</scene>, resolution 1.90&Aring;
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<StructureSection load='2fbd' size='340' side='right'caption='[[2fbd]], [[Resolution|resolution]] 1.90&Aring;' scene=''>
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|SITE=
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== Structural highlights ==
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|LIGAND= <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene> and <scene name='pdbligand=PEG:DI(HYDROXYETHYL)ETHER'>PEG</scene>
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<table><tr><td colspan='2'>[[2fbd]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Musca_domestica Musca domestica]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2FBD OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2FBD FirstGlance]. <br>
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|ACTIVITY= [http://en.wikipedia.org/wiki/Lysozyme Lysozyme], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.17 3.2.1.17]
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.9&#8491;</td></tr>
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|GENE= AY344589 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=7370 Musca domestica])
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=PEG:DI(HYDROXYETHYL)ETHER'>PEG</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
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}}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2fbd FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2fbd OCA], [https://pdbe.org/2fbd PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2fbd RCSB], [https://www.ebi.ac.uk/pdbsum/2fbd PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2fbd ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/LYS1_MUSDO LYS1_MUSDO] May not function as a self-defense protein, but as a digestive enzyme, probably in the gut of the insect body. Inactive towards Micrococcus luteus. Active toward glycol chitin.
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/fb/2fbd_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2fbd ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Lysozymes are mostly known for their defensive role against bacteria, but in several animals lysozymes have a digestive function. Here, the initial crystallographic characterization of two digestive lysozymes from Musca domestica are presented. The proteins were crystallized using the sitting-drop vapour-diffusion method in the presence of ammonium sulfate or PEG/2-propanol as the precipitant. X-ray diffraction data were collected to a maximum resolution of 1.9 angstroms using synchrotron radiation. The lysozyme 1 and 2 crystals belong to the monoclinic space group P2(1) (unit-cell parameters a = 36.52, b = 79.44, c = 45.20 angstroms, beta = 102.97 degrees) and the orthorhombic space group P2(1)2(1)2 (unit-cell parameters a = 73.90, b = 96.40, c = 33.27 angstroms), respectively. The crystal structures were solved by molecular replacement and structure refinement is in progress.
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'''The crystallographic structure of the digestive lysozyme 1 from Musca domestica at 1.90 Ang.'''
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Crystallization, data collection and phasing of two digestive lysozymes from Musca domestica.,Marana SR, Cancado FC, Valerio AA, Ferreira C, Terra WR, Barbosa JA Acta Crystallogr Sect F Struct Biol Cryst Commun. 2006 Aug 1;62(Pt, 8):750-2. Epub 2006 Jul 24. PMID:16880547<ref>PMID:16880547</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 2fbd" style="background-color:#fffaf0;"></div>
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==Overview==
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==See Also==
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Lysozymes are mostly known for their defensive role against bacteria, but in several animals lysozymes have a digestive function. Here, the initial crystallographic characterization of two digestive lysozymes from Musca domestica are presented. The proteins were crystallized using the sitting-drop vapour-diffusion method in the presence of ammonium sulfate or PEG/2-propanol as the precipitant. X-ray diffraction data were collected to a maximum resolution of 1.9 angstroms using synchrotron radiation. The lysozyme 1 and 2 crystals belong to the monoclinic space group P2(1) (unit-cell parameters a = 36.52, b = 79.44, c = 45.20 angstroms, beta = 102.97 degrees) and the orthorhombic space group P2(1)2(1)2 (unit-cell parameters a = 73.90, b = 96.40, c = 33.27 angstroms), respectively. The crystal structures were solved by molecular replacement and structure refinement is in progress.
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*[[Lysozyme 3D structures|Lysozyme 3D structures]]
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== References ==
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==About this Structure==
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<references/>
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2FBD is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Musca_domestica Musca domestica]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2FBD OCA].
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__TOC__
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</StructureSection>
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==Reference==
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[[Category: Large Structures]]
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Crystallization, data collection and phasing of two digestive lysozymes from Musca domestica., Marana SR, Cancado FC, Valerio AA, Ferreira C, Terra WR, Barbosa JA, Acta Crystallogr Sect F Struct Biol Cryst Commun. 2006 Aug 1;62(Pt, 8):750-2. Epub 2006 Jul 24. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/16880547 16880547]
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[[Category: Lysozyme]]
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[[Category: Musca domestica]]
[[Category: Musca domestica]]
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[[Category: Single protein]]
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[[Category: Barbosa JARG]]
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[[Category: Barbosa, J A.R G.]]
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[[Category: Cancado FC]]
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[[Category: Cancado, F C.]]
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[[Category: Marana SR]]
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[[Category: Marana, S R.]]
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[[Category: PEG]]
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[[Category: SO4]]
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[[Category: digestive lysozime]]
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[[Category: musca domestica]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 16:50:21 2008''
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Current revision

The crystallographic structure of the digestive lysozyme 1 from Musca domestica at 1.90 Ang.

PDB ID 2fbd

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