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2fco

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[[Image:2fco.gif|left|200px]]
 
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{{Structure
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==Crystal Structure of Bacillus stearothermophilus PrfA-Holliday Junction Resolvase==
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|PDB= 2fco |SIZE=350|CAPTION= <scene name='initialview01'>2fco</scene>, resolution 1.40&Aring;
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<StructureSection load='2fco' size='340' side='right'caption='[[2fco]], [[Resolution|resolution]] 1.40&Aring;' scene=''>
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|SITE=
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== Structural highlights ==
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|LIGAND= <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene> and <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>
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<table><tr><td colspan='2'>[[2fco]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Geobacillus_kaustophilus_HTA426 Geobacillus kaustophilus HTA426]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2FCO OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2FCO FirstGlance]. <br>
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|ACTIVITY= [http://en.wikipedia.org/wiki/Crossover_junction_endodeoxyribonuclease Crossover junction endodeoxyribonuclease], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.22.4 3.1.22.4]
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.4&#8491;</td></tr>
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|GENE= RecU/PrfA ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1462 Geobacillus kaustophilus])
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
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}}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2fco FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2fco OCA], [https://pdbe.org/2fco PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2fco RCSB], [https://www.ebi.ac.uk/pdbsum/2fco PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2fco ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/RECU_GEOKA RECU_GEOKA] Endonuclease that resolves Holliday junction intermediates in genetic recombination. Cleaves mobile four-strand junctions by introducing symmetrical nicks in paired strands. Promotes annealing of linear ssDNA with homologous dsDNA. Required for DNA repair, homologous recombination and chromosome segregation.[HAMAP-Rule:MF_00130]<ref>PMID:12237459</ref>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/fc/2fco_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2fco ConSurf].
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<div style="clear:both"></div>
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'''Crystal Structure of Bacillus stearothermophilus PrfA-Holliday Junction Resolvase'''
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==See Also==
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*[[Resolvase 3D structures|Resolvase 3D structures]]
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== References ==
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==Overview==
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<references/>
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Here we report a high resolution structure of RecU-Holliday junction resolvase from Bacillus stearothermophilus. The functional unit of RecU is a homodimer that contains a "mushroom" like structure with a rigid cap and two highly flexible loops extending outwards. These loops appear to be highly flexible/dynamic, and presumably are directly involved in DNA binding and holding it for catalysis. Structural modifications of both the protein and DNA upon their interaction are essential for catalysis. An Mg2+ ion is present in each of the two active sites in this homodimeric enzyme, and two water molecules are coordinated with each Mg2+ ion. Our data are consistent with one of these water molecules acting as a nucleophile and the other as a general acid. The identities of the general base and general acid involved in catalysis and the Lewis acid that stabilizes the pentacovalent transition state phosphate ion are proposed. A model for the RecU-Holliday junction DNA complex is also proposed and discussed in the context of DNA binding and cleavage.
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__TOC__
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</StructureSection>
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==About this Structure==
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[[Category: Geobacillus kaustophilus HTA426]]
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2FCO is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Geobacillus_kaustophilus Geobacillus kaustophilus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2FCO OCA].
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[[Category: Large Structures]]
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[[Category: Jedrzejas MJ]]
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==Reference==
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[[Category: Li J]]
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Structure, flexibility, and mechanism of the Bacillus stearothermophilus RecU Holliday junction resolvase., Kelly SJ, Li J, Setlow P, Jedrzejas MJ, Proteins. 2007 Sep 1;68(4):961-71. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/17557334 17557334]
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[[Category: Crossover junction endodeoxyribonuclease]]
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[[Category: Geobacillus kaustophilus]]
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[[Category: Single protein]]
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[[Category: Jedrzejas, M J.]]
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[[Category: Li, J.]]
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[[Category: EDO]]
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[[Category: MG]]
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[[Category: crystal structure]]
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[[Category: flexibility]]
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[[Category: hydrolase]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 16:50:49 2008''
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Current revision

Crystal Structure of Bacillus stearothermophilus PrfA-Holliday Junction Resolvase

PDB ID 2fco

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