2jr5
From Proteopedia
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==Solution structure of UPF0350 protein VC_2471. Northeast Structural Genomics Target VcR36== | ==Solution structure of UPF0350 protein VC_2471. Northeast Structural Genomics Target VcR36== | ||
| - | <StructureSection load='2jr5' size='340' side='right' caption='[[2jr5 | + | <StructureSection load='2jr5' size='340' side='right'caption='[[2jr5]]' scene=''> |
== Structural highlights == | == Structural highlights == | ||
| - | <table><tr><td colspan='2'>[[2jr5]] is a 1 chain structure with sequence from [ | + | <table><tr><td colspan='2'>[[2jr5]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Vibrio_cholerae Vibrio cholerae]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2JR5 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2JR5 FirstGlance]. <br> |
| - | </td></tr><tr id=' | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[ | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2jr5 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2jr5 OCA], [https://pdbe.org/2jr5 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2jr5 RCSB], [https://www.ebi.ac.uk/pdbsum/2jr5 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2jr5 ProSAT]</span></td></tr> |
</table> | </table> | ||
| + | == Function == | ||
| + | [https://www.uniprot.org/uniprot/SDHE_VIBCH SDHE_VIBCH] An FAD assembly protein, which accelerates covalent attachment of the cofactor into other proteins. Plays an essential role in the assembly of succinate dehydrogenase (SDH, respiratory complex II), an enzyme complex that is a component of both the tricarboxylic acid cycle and the electron transport chain, and which couples the oxidation of succinate to fumarate with the reduction of ubiquinone (coenzyme Q) to ubiquinol. Required for flavinylation (covalent attachment of FAD) of the flavoprotein subunit SdhA of SDH and other flavinylated proteins as well.[UniProtKB:G4V4G2] | ||
== Evolutionary Conservation == | == Evolutionary Conservation == | ||
[[Image:Consurf_key_small.gif|200px|right]] | [[Image:Consurf_key_small.gif|200px|right]] | ||
Check<jmol> | Check<jmol> | ||
<jmolCheckbox> | <jmolCheckbox> | ||
| - | <scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/jr/2jr5_consurf.spt"</scriptWhenChecked> | + | <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/jr/2jr5_consurf.spt"</scriptWhenChecked> |
<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked> | <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked> | ||
<text>to colour the structure by Evolutionary Conservation</text> | <text>to colour the structure by Evolutionary Conservation</text> | ||
</jmolCheckbox> | </jmolCheckbox> | ||
| - | </jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/ | + | </jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2jr5 ConSurf]. |
<div style="clear:both"></div> | <div style="clear:both"></div> | ||
| - | <div style="background-color:#fffaf0;"> | ||
| - | == Publication Abstract from PubMed == | ||
| - | The yeast mitochondrial protein Sdh5 is required for the covalent attachment of flavin adenine dinucleotide (FAD) to protein Sdh1, a subunit of the heterotetrameric enzyme succinate dehydrogenase. The NMR structure of Sdh5 represents the first eukaryotic structure of Pfam family PF03937 and reveals a conserved surface region, which likely represents a putative Sdh1-Sdh5 interaction interface. Point mutations in this region result in the loss of covalent flavinylation of Sdh1. Moreover, chemical shift perturbation measurements showed that Sdh5 does not bind FAD in vitro, indicating that it is not a simple cofactor transporter in vivo. | ||
| - | |||
| - | Solution NMR Structure of Yeast Succinate Dehydrogenase Flavinylation Factor Sdh5 Reveals a Putative Sdh1 Binding Site.,Eletsky A, Jeong MY, Kim H, Lee HW, Xiao R, Pagliarini DJ, Prestegard JH, Winge DR, Montelione GT, Szyperski T Biochemistry. 2012 Oct 19. PMID:23062074<ref>PMID:23062074</ref> | ||
| - | |||
| - | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
| - | </div> | ||
| - | == References == | ||
| - | <references/> | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
| + | [[Category: Large Structures]] | ||
[[Category: Vibrio cholerae]] | [[Category: Vibrio cholerae]] | ||
| - | [[Category: Acton | + | [[Category: Acton TB]] |
| - | [[Category: Baran | + | [[Category: Baran MC]] |
| - | [[Category: Eletski | + | [[Category: Eletski A]] |
| - | [[Category: Liu | + | [[Category: Liu J]] |
| - | [[Category: Maglaqui | + | [[Category: Maglaqui M]] |
| - | [[Category: Montelione | + | [[Category: Montelione GT]] |
| - | + | [[Category: Nwosu C]] | |
| - | [[Category: Nwosu | + | [[Category: Parish D]] |
| - | [[Category: Parish | + | [[Category: Rost B]] |
| - | [[Category: Rost | + | [[Category: Shastry R]] |
| - | [[Category: Shastry | + | [[Category: Singarapu KK]] |
| - | [[Category: Singarapu | + | [[Category: Sukumaran D]] |
| - | [[Category: Sukumaran | + | [[Category: Swapna G]] |
| - | [[Category: Swapna | + | [[Category: Szyperski T]] |
| - | [[Category: Szyperski | + | [[Category: Wu Y]] |
| - | [[Category: Wu | + | [[Category: Xiao R]] |
| - | [[Category: Xiao | + | |
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Current revision
Solution structure of UPF0350 protein VC_2471. Northeast Structural Genomics Target VcR36
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Categories: Large Structures | Vibrio cholerae | Acton TB | Baran MC | Eletski A | Liu J | Maglaqui M | Montelione GT | Nwosu C | Parish D | Rost B | Shastry R | Singarapu KK | Sukumaran D | Swapna G | Szyperski T | Wu Y | Xiao R
