2fgq

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[[Image:2fgq.gif|left|200px]]
 
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{{Structure
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==High resolution X-ray structure of Omp32 in complex with malate==
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|PDB= 2fgq |SIZE=350|CAPTION= <scene name='initialview01'>2fgq</scene>, resolution 1.450&Aring;
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<StructureSection load='2fgq' size='340' side='right'caption='[[2fgq]], [[Resolution|resolution]] 1.45&Aring;' scene=''>
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|SITE=
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== Structural highlights ==
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|LIGAND= <scene name='pdbligand=BOG:B-OCTYLGLUCOSIDE'>BOG</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene> and <scene name='pdbligand=MLT:MALATE ION'>MLT</scene>
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<table><tr><td colspan='2'>[[2fgq]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Delftia_acidovorans Delftia acidovorans]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2FGQ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2FGQ FirstGlance]. <br>
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|ACTIVITY=
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.45&#8491;</td></tr>
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|GENE=
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BOG:B-OCTYLGLUCOSIDE'>BOG</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=MLT:D-MALATE'>MLT</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
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}}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2fgq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2fgq OCA], [https://pdbe.org/2fgq PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2fgq RCSB], [https://www.ebi.ac.uk/pdbsum/2fgq PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2fgq ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/OMP32_DELAC OMP32_DELAC]
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/fg/2fgq_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2fgq ConSurf].
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<div style="clear:both"></div>
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'''High resolution X-ray structure of Omp32 in complex with malate'''
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==See Also==
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*[[Porin 3D structures|Porin 3D structures]]
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__TOC__
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==Overview==
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</StructureSection>
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The porin Omp32 is the major outer membrane protein of the bacterium Delftia acidovorans. The crystal structures of the strongly anion-selective porin alone and in complex with the substrate malate were solved at 1.5 and 1.45 A resolution, respectively, and revealed a malate-binding motif adjacent to the channel constriction zone. Binding is mediated by interaction with a cluster of two arginine residues and two threonines. This binding site is specific for Omp32 and reflects the physiological adaptation of the organism to organic acids. Structural studies are combined with a 7-ns unbiased molecular dynamics simulation of the trimeric channel in a model membrane. Molecular dynamics trajectories show how malate ions are efficiently captured from the surrounding bulk solution by the electrostatic potential of the channel, translocated to the binding site region, and immobilized in the constriction zone. In accordance with these results, conductance measurements with Omp32 inserted in planar lipid membranes revealed binding of malate. The anion-selective channel Omp32 is the first reported example of a porin with a 16-stranded beta-barrel and proven substrate specificity. This finding suggests a new view on the correlation of porin structure with substrate binding in specific channels.
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==About this Structure==
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2FGQ is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Delftia_acidovorans Delftia acidovorans]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2FGQ OCA].
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==Reference==
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High resolution crystal structures and molecular dynamics studies reveal substrate binding in the porin Omp32., Zachariae U, Kluhspies T, De S, Engelhardt H, Zeth K, J Biol Chem. 2006 Mar 17;281(11):7413-20. Epub 2006 Jan 23. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/16434398 16434398]
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[[Category: Delftia acidovorans]]
[[Category: Delftia acidovorans]]
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[[Category: Single protein]]
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[[Category: Large Structures]]
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[[Category: Engelhardt, H.]]
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[[Category: Engelhardt H]]
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[[Category: Zeth, K.]]
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[[Category: Zeth K]]
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[[Category: BOG]]
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[[Category: CA]]
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[[Category: MLT]]
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[[Category: SO4]]
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[[Category: malate]]
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[[Category: outer membrane protein]]
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[[Category: porin]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 16:52:14 2008''
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Current revision

High resolution X-ray structure of Omp32 in complex with malate

PDB ID 2fgq

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