2fi7

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[[Image:2fi7.gif|left|200px]]
 
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{{Structure
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==Crystal Structure of PilF : Functional implication in the type 4 pilus biogenesis in Pseudomonas aeruginosa==
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|PDB= 2fi7 |SIZE=350|CAPTION= <scene name='initialview01'>2fi7</scene>, resolution 2.2&Aring;
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<StructureSection load='2fi7' size='340' side='right'caption='[[2fi7]], [[Resolution|resolution]] 2.20&Aring;' scene=''>
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|SITE=
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== Structural highlights ==
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|LIGAND=
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<table><tr><td colspan='2'>[[2fi7]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Pseudomonas_aeruginosa_PAO1 Pseudomonas aeruginosa PAO1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2FI7 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2FI7 FirstGlance]. <br>
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|ACTIVITY=
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.2&#8491;</td></tr>
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|GENE=
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2fi7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2fi7 OCA], [https://pdbe.org/2fi7 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2fi7 RCSB], [https://www.ebi.ac.uk/pdbsum/2fi7 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2fi7 ProSAT]</span></td></tr>
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}}
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</table>
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== Function ==
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'''Crystal Structure of PilF : Functional implication in the type 4 pilus biogenesis in Pseudomonas aeruginosa'''
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[https://www.uniprot.org/uniprot/PILF_PSEAE PILF_PSEAE] Essential component of the type IV pilus (T4P) that plays a role in surface and host cell adhesion, colonization, biofilm maturation, virulence, and twitching, a form of surface-associated motility facilitated by cycles of extension, adhesion, and retraction of T4P fibers (PubMed:8973346). Plays an essential role in the outer membrane localization and assembly of PilQ into secretins which are dodecamers of PilQ (PubMed:23547883, PubMed:18776008).<ref>PMID:18776008</ref> <ref>PMID:23547883</ref> <ref>PMID:8973346</ref>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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==Overview==
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Check<jmol>
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PilF is a requisite protein involved in the type 4 pilus biogenesis system from the Gram-negative human pathogenic bacteria, Pseudomonas aeruginosa. We determined the PilF structure at a 2.2A resolution; this includes six tandem tetratrico peptide repeat (TPR) units forming right-handed superhelix. PilF structure was similar to the heat shock protein organizing protein, which interacts with the C-terminal peptide of Hsp90 and Hsp70 via a concave Asn ladder in the inner groove of TPR superhelix. After simulated screening, the C-terminal pentapeptides of PilG, PilU, PilY, and PilZ proved to be a likely candidate binding to PilF, which are ones of 25 necessary components involved in the type 4 pilus biogenesis system. We proposed that PilF would be critical as a bridgehead in protein-protein interaction and thereby, PilF may bind a necessary molecule in type 4 pilus biogenesis system such as PilG, PilU, PilY, and PilZ.
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/fi/2fi7_consurf.spt"</scriptWhenChecked>
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==About this Structure==
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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2FI7 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Pseudomonas_aeruginosa Pseudomonas aeruginosa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2FI7 OCA].
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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==Reference==
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2fi7 ConSurf].
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Crystal structure of PilF: functional implication in the type 4 pilus biogenesis in Pseudomonas aeruginosa., Kim K, Oh J, Han D, Kim EE, Lee B, Kim Y, Biochem Biophys Res Commun. 2006 Feb 24;340(4):1028-38. Epub 2005 Dec 27. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/16403447 16403447]
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<div style="clear:both"></div>
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[[Category: Pseudomonas aeruginosa]]
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== References ==
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[[Category: Single protein]]
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<references/>
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[[Category: Kim, K.]]
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__TOC__
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[[Category: crystal structure]]
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</StructureSection>
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[[Category: pilf]]
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[[Category: Large Structures]]
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[[Category: pseudomonas aeruginosa]]
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[[Category: Pseudomonas aeruginosa PAO1]]
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[[Category: tpr]]
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[[Category: Kim K]]
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[[Category: type 4 pilus]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 16:52:41 2008''
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Current revision

Crystal Structure of PilF : Functional implication in the type 4 pilus biogenesis in Pseudomonas aeruginosa

PDB ID 2fi7

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