2fj7

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[[Image:2fj7.gif|left|200px]]
 
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{{Structure
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==Crystal structure of Nucleosome Core Particle Containing a Poly (dA.dT) Sequence Element==
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|PDB= 2fj7 |SIZE=350|CAPTION= <scene name='initialview01'>2fj7</scene>, resolution 3.2&Aring;
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<StructureSection load='2fj7' size='340' side='right'caption='[[2fj7]], [[Resolution|resolution]] 3.20&Aring;' scene=''>
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|SITE=
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== Structural highlights ==
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|LIGAND=
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<table><tr><td colspan='2'>[[2fj7]] is a 10 chain structure with sequence from [https://en.wikipedia.org/wiki/Xenopus_laevis Xenopus laevis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2FJ7 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2FJ7 FirstGlance]. <br>
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|ACTIVITY=
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3.2&#8491;</td></tr>
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|GENE=
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2fj7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2fj7 OCA], [https://pdbe.org/2fj7 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2fj7 RCSB], [https://www.ebi.ac.uk/pdbsum/2fj7 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2fj7 ProSAT]</span></td></tr>
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}}
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/H32_XENLA H32_XENLA] Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/fj/2fj7_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2fj7 ConSurf].
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<div style="clear:both"></div>
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'''Crystal structure of Nucleosome Core Particle Containing a Poly (dA.dT) Sequence Element'''
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==See Also==
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*[[Histone 3D structures|Histone 3D structures]]
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__TOC__
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==Overview==
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</StructureSection>
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Poly(dA.dT) DNA sequence elements are thought to promote transcription by either excluding nucleosomes or by altering their structural or dynamic properties. Here, the stability and structure of a defined nucleosome core particle containing a 16 base-pair poly(dA.dT) element (A16 NCP) was investigated. The A16 NCP requires a significantly higher temperature for histone octamer sliding in vitro compared to comparable nucleosomes that do not contain a poly(dA.dT) element. Fluorescence resonance energy transfer showed that the interactions between the nucleosomal DNA ends and the histone octamer were destabilized in A16 NCP. The crystal structure of A16 NCP was determined to a resolution of 3.2 A. The overall structure was maintained except for local deviations in DNA conformation. These results are consistent with previous in vivo and in vitro observations that poly(dA.dT) elements cause only modest changes in DNA accessibility and modest increases in steady-state transcription levels.
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[[Category: Large Structures]]
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==About this Structure==
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2FJ7 is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/Xenopus_laevis Xenopus laevis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2FJ7 OCA].
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==Reference==
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Nucleosome core particles containing a poly(dA.dT) sequence element exhibit a locally distorted DNA structure., Bao Y, White CL, Luger K, J Mol Biol. 2006 Aug 25;361(4):617-24. Epub 2006 Jul 5. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/16860337 16860337]
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[[Category: Protein complex]]
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[[Category: Xenopus laevis]]
[[Category: Xenopus laevis]]
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[[Category: Bao, Y.]]
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[[Category: Bao Y]]
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[[Category: Luger, K.]]
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[[Category: Luger K]]
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[[Category: White, C L.]]
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[[Category: White CL]]
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[[Category: narrow minor groove]]
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[[Category: protein-dna complex]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 16:53:03 2008''
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Current revision

Crystal structure of Nucleosome Core Particle Containing a Poly (dA.dT) Sequence Element

PDB ID 2fj7

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