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3zqe

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PrgI-SipD from Salmonella typhimurium in complex with deoxycholate

Structural highlights

3zqe is a 2 chain structure with sequence from Salmonella enterica subsp. enterica serovar Typhimurium str. SL1344. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:	X-ray diffraction, Resolution 2.19Å
Ligands:	,
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

SIPD_SALTY Required for translocation of effector proteins via the type III secretion system SPI-1, which is essential for an efficient bacterial internalization. Probably acts by modulating the secretion of SipA, SipB, and SipC.^[1] ^[2] PRGI_SALTY Required for invasion of epithelial cells.

Publication Abstract from PubMed

Many infectious gram-negative bacteria, including Salmonella typhimurium, require a Type Three Secretion System (T3SS) to translocate virulence factors into host cells. The T3SS consists of a membrane protein complex and an extracellular needle together that form a continuous channel. Regulated secretion of virulence factors requires the presence of SipD at the T3SS needle tip in S. typhimurium. Here we report three-dimensional structures of individual SipD, SipD in fusion with the needle subunit PrgI, and of SipD:PrgI in complex with the bile salt, deoxycholate. Assembly of the complex involves major conformational changes in both SipD and PrgI. This rearrangement is mediated via a pi bulge in the central SipD helix and is stabilized by conserved amino acids that may allow for specificity in the assembly and composition of the tip proteins. Five copies each of the needle subunit PrgI and SipD form the T3SS needle tip complex. Using surface plasmon resonance spectroscopy and crystal structure analysis we found that the T3SS needle tip complex binds deoxycholate with micromolar affinity via a cleft formed at the SipD:PrgI interface. In the structure-based three-dimensional model of the T3SS needle tip, the bound deoxycholate faces the host membrane. Recently, binding of SipD with bile salts present in the gut was shown to impede bacterial infection. Binding of bile salts to the SipD:PrgI interface in this particular arrangement may thus inhibit the T3SS function. The structures presented in this study provide insight into the open state of the T3SS needle tip. Our findings present the atomic details of the T3SS arrangement occurring at the pathogen-host interface.

Crystal structure of PrgI-SipD: insight into a secretion competent state of the type three secretion system needle tip and its interaction with host ligands.,Lunelli M, Hurwitz R, Lambers J, Kolbe M PLoS Pathog. 2011 Aug;7(8):e1002163. Epub 2011 Aug 4. PMID:21829362^[3]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Kaniga K, Trollinger D, Galan JE. Identification of two targets of the type III protein secretion system encoded by the inv and spa loci of Salmonella typhimurium that have homology to the Shigella IpaD and IpaA proteins. J Bacteriol. 1995 Dec;177(24):7078-85. PMID:8522512
↑ Collazo CM, Galan JE. The invasion-associated type III system of Salmonella typhimurium directs the translocation of Sip proteins into the host cell. Mol Microbiol. 1997 May;24(4):747-56. PMID:9194702
↑ Lunelli M, Hurwitz R, Lambers J, Kolbe M. Crystal structure of PrgI-SipD: insight into a secretion competent state of the type three secretion system needle tip and its interaction with host ligands. PLoS Pathog. 2011 Aug;7(8):e1002163. Epub 2011 Aug 4. PMID:21829362 doi:10.1371/journal.ppat.1002163

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/3zqe"

Categories: Large Structures | Salmonella enterica subsp. enterica serovar Typhimurium str. SL1344 | Kolbe M | Lunelli M

@@ Line 1: / Line 1: @@
 ==PrgI-SipD from Salmonella typhimurium in complex with deoxycholate==
-<StructureSection load='3zqe' size='340' side='right' caption='[[3zqe]], [[Resolution|resolution]] 2.19&Aring;' scene=''>
+<StructureSection load='3zqe' size='340' side='right'caption='[[3zqe]], [[Resolution|resolution]] 2.19&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[3zqe]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Salmonella_enterica_subsp._enterica_serovar_typhimurium Salmonella enterica subsp. enterica serovar typhimurium]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3ZQE OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3ZQE FirstGlance]. <br>
+<table><tr><td colspan='2'>[[3zqe]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Salmonella_enterica_subsp._enterica_serovar_Typhimurium_str._SL1344 Salmonella enterica subsp. enterica serovar Typhimurium str. SL1344]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3ZQE OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3ZQE FirstGlance]. <br>
-</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=DXC:(3ALPHA,5BETA,12ALPHA)-3,12-DIHYDROXYCHOLAN-24-OIC+ACID'>DXC</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.19&#8491;</td></tr>
-<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[2ym9|2ym9]], [[2ym0|2ym0]], [[2x9c|2x9c]], [[3zqb|3zqb]]</td></tr>
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=DXC:(3ALPHA,5BETA,12ALPHA)-3,12-DIHYDROXYCHOLAN-24-OIC+ACID'>DXC</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3zqe FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3zqe OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3zqe RCSB], [http://www.ebi.ac.uk/pdbsum/3zqe PDBsum]</span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3zqe FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3zqe OCA], [https://pdbe.org/3zqe PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3zqe RCSB], [https://www.ebi.ac.uk/pdbsum/3zqe PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3zqe ProSAT]</span></td></tr>
 </table>
 == Function ==
-[[http://www.uniprot.org/uniprot/PRGI_SALTY PRGI_SALTY]] Required for invasion of epithelial cells.
+[https://www.uniprot.org/uniprot/SIPD_SALTY SIPD_SALTY] Required for translocation of effector proteins via the type III secretion system SPI-1, which is essential for an efficient bacterial internalization. Probably acts by modulating the secretion of SipA, SipB, and SipC.<ref>PMID:8522512</ref> <ref>PMID:9194702</ref> [https://www.uniprot.org/uniprot/PRGI_SALTY PRGI_SALTY] Required for invasion of epithelial cells.
 <div style="background-color:#fffaf0;">
 == Publication Abstract from PubMed ==
@@ Line 17: / Line 18: @@
 From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
 </div>
+<div class="pdbe-citations 3zqe" style="background-color:#fffaf0;"></div>
 == References ==
 <references/>
 __TOC__
 </StructureSection>
-[[Category: Salmonella enterica subsp. enterica serovar typhimurium]]
+[[Category: Large Structures]]
-[[Category: Kolbe, M]]
+[[Category: Salmonella enterica subsp. enterica serovar Typhimurium str. SL1344]]
-[[Category: Lunelli, M]]
+[[Category: Kolbe M]]
-[[Category: Bacterial pathogenesis]]
+[[Category: Lunelli M]]
-[[Category: Bile salt]]
-[[Category: Cell invasion]]
-[[Category: Cell invasion complex]]
-[[Category: Host pathogen interaction]]
-[[Category: T3ss]]
-[[Category: Tip complex]]
-[[Category: Type iii secretion]]

3zqe

From Proteopedia

Current revision

PrgI-SipD from Salmonella typhimurium in complex with deoxycholate

Structural highlights

Function

Publication Abstract from PubMed

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

Views

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