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1rcx

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(New page: 200px<br /> <applet load="1rcx" size="450" color="white" frame="true" align="right" spinBox="true" caption="1rcx, resolution 2.4&Aring;" /> '''NON-ACTIVATED SPINAC...)
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[[Image:1rcx.gif|left|200px]]<br />
 
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<applet load="1rcx" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1rcx, resolution 2.4&Aring;" />
 
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'''NON-ACTIVATED SPINACH RUBISCO IN COMPLEX WITH ITS SUBSTRATE RIBULOSE-1,5-BISPHOSPHATE'''<br />
 
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==Overview==
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==NON-ACTIVATED SPINACH RUBISCO IN COMPLEX WITH ITS SUBSTRATE RIBULOSE-1,5-BISPHOSPHATE==
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The three-dimensional structure of the complex of ribulose, 1,5-bisphosphate carboxylase/oxygenase (rubisco; EC 4.1.1.39) from spinach, with its natural substrate ribulose 1,5-bisphosphate (RuBP) has been, determined both under activating and non-activating conditions by X-ray, crystallography to a resolution of 2.1 A and 2.4 A, respectively. Under, activating conditions, the use of calcium instead of magnesium as the, activator metal ion enabled us to trap the substrate in a stable complex, for crystallographic analysis. Comparison of the structure of the, activated and the non-activated RuBP complexes shows a tighter binding for, the substrate in the non-activated form of the enzyme, in line with, previous solution studies. In the non-activated complex, the substrate, triggers isolation ... [[http://ispc.weizmann.ac.il/pmbin/getpm?9034362 (full description)]]
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<StructureSection load='1rcx' size='340' side='right'caption='[[1rcx]], [[Resolution|resolution]] 2.40&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[1rcx]] is a 16 chain structure with sequence from [https://en.wikipedia.org/wiki/Spinacia_oleracea Spinacia oleracea]. The November 2000 RCSB PDB [https://pdb.rcsb.org/pdb/static.do?p=education_discussion/molecule_of_the_month/index.html Molecule of the Month] feature on ''Rubisco'' by David S. Goodsell is [https://dx.doi.org/10.2210/rcsb_pdb/mom_2000_11 10.2210/rcsb_pdb/mom_2000_11]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1RCX OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1RCX FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.4&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=RUB:RIBULOSE-1,5-DIPHOSPHATE'>RUB</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1rcx FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1rcx OCA], [https://pdbe.org/1rcx PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1rcx RCSB], [https://www.ebi.ac.uk/pdbsum/1rcx PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1rcx ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/RBL_SPIOL RBL_SPIOL] RuBisCO catalyzes two reactions: the carboxylation of D-ribulose 1,5-bisphosphate, the primary event in carbon dioxide fixation, as well as the oxidative fragmentation of the pentose substrate in the photorespiration process. Both reactions occur simultaneously and in competition at the same active site.[HAMAP-Rule:MF_01338]
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/rc/1rcx_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1rcx ConSurf].
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<div style="clear:both"></div>
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==About this Structure==
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==See Also==
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1RCX is a [[http://en.wikipedia.org/wiki/Protein_complex Protein complex]] structure of sequences from [[http://en.wikipedia.org/wiki/Spinacia_oleracea Spinacia oleracea]] with RUB as [[http://en.wikipedia.org/wiki/ligand ligand]]. The following page contains interesting information on the relation of 1RCX with [[http://pdb.rcsb.org/pdb/static.do?p=education_discussion/molecule_of_the_month/pdb11_1.html Rubisco]]. Active as [[http://en.wikipedia.org/wiki/ ]], with EC number [[http://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.1.1.39 4.1.1.39]]. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1RCX OCA]].
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*[[RuBisCO|RuBisCO]]
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*[[RuBisCO 3D structures|RuBisCO 3D structures]]
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==Reference==
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__TOC__
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The structure of the complex between rubisco and its natural substrate ribulose 1,5-bisphosphate., Taylor TC, Andersson I, J Mol Biol. 1997 Jan 31;265(4):432-44. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=9034362 9034362]
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</StructureSection>
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[[Category: Protein complex]]
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[[Category: Large Structures]]
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[[Category: RCSB PDB Molecule of the Month]]
[[Category: Rubisco]]
[[Category: Rubisco]]
[[Category: Spinacia oleracea]]
[[Category: Spinacia oleracea]]
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[[Category: Andersson, I.]]
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[[Category: Andersson I]]
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[[Category: Taylor, T.C.]]
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[[Category: Taylor TC]]
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[[Category: RUB]]
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[[Category: lyase (carbon-carbon)]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Mon Oct 29 16:57:50 2007''
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Current revision

NON-ACTIVATED SPINACH RUBISCO IN COMPLEX WITH ITS SUBSTRATE RIBULOSE-1,5-BISPHOSPHATE

PDB ID 1rcx

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