2fwv

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[[Image:2fwv.gif|left|200px]]
 
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{{Structure
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==Crystal Structure of Rv0813==
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|PDB= 2fwv |SIZE=350|CAPTION= <scene name='initialview01'>2fwv</scene>, resolution 1.700&Aring;
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<StructureSection load='2fwv' size='340' side='right'caption='[[2fwv]], [[Resolution|resolution]] 1.70&Aring;' scene=''>
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|SITE=
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== Structural highlights ==
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|LIGAND= <scene name='pdbligand=DIO:1,4-DIETHYLENE+DIOXIDE'>DIO</scene> and <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>
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<table><tr><td colspan='2'>[[2fwv]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Mycobacterium_tuberculosis_H37Rv Mycobacterium tuberculosis H37Rv]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2FWV OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2FWV FirstGlance]. <br>
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|ACTIVITY=
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.7&#8491;</td></tr>
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|GENE= rv0813c ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1773 Mycobacterium tuberculosis])
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=DIO:1,4-DIETHYLENE+DIOXIDE'>DIO</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr>
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}}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2fwv FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2fwv OCA], [https://pdbe.org/2fwv PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2fwv RCSB], [https://www.ebi.ac.uk/pdbsum/2fwv PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2fwv ProSAT]</span></td></tr>
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</table>
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'''Crystal Structure of Rv0813'''
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== Function ==
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[https://www.uniprot.org/uniprot/NB2_MYCTU NB2_MYCTU] Heme-binding protein able to scavenge peroxynitrite and to protect free L-tyrosine against peroxynitrite-mediated nitration, by acting as a peroxynitrite isomerase that converts peroxynitrite to nitrate. Therefore, this protein likely plays a role in peroxynitrite sensing and in the detoxification of reactive nitrogen and oxygen species (RNS and ROS, respectively). Is able to bind nitric oxide (NO) in vitro, but may act as a sensor of peroxynitrite levels in vivo.[HAMAP-Rule:MF_01297]
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== Evolutionary Conservation ==
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==Overview==
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[[Image:Consurf_key_small.gif|200px|right]]
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The gene Rv0813c from Mycobacterium tuberculosis, which codes for a hypothetical protein of unknown function, is conserved within the order Actinomycetales but absent elsewhere. The crystal structure of Rv0813c reveals a new family of proteins that resemble the fatty acid-binding proteins (FABPs) found in eukaryotes. Rv0813c adopts the 10-stranded beta-barrel fold typical of FABPs but lacks the double-helix insert that covers the entry to the binding site in the eukaryotic proteins. The barrel encloses a deep cavity, at the bottom of which a small cyclic ligand was found to bind to the hydroxyl group of Tyr192. This residue is part of a conserved Arg-X-Tyr motif much like the triad that binds the carboxylate group of fatty acids in FABPs. Most of the residues forming the internal surface of the cavity are conserved in homologous protein sequences found in CG-rich prokaryotes, strongly suggesting that Rv0813c is a member of a new family of bacterial FABP-like proteins that may have roles in the recognition, transport, and/or storage of small molecules in the bacterial cytosol.
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Check<jmol>
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<jmolCheckbox>
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==About this Structure==
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/fw/2fwv_consurf.spt"</scriptWhenChecked>
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2FWV is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Mycobacterium_tuberculosis Mycobacterium tuberculosis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2FWV OCA].
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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==Reference==
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</jmolCheckbox>
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The crystal structure of Rv0813c from Mycobacterium tuberculosis reveals a new family of fatty acid-binding protein-like proteins in bacteria., Shepard W, Haouz A, Grana M, Buschiazzo A, Betton JM, Cole ST, Alzari PM, J Bacteriol. 2007 Mar;189(5):1899-904. Epub 2006 Dec 15. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/17172346 17172346]
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2fwv ConSurf].
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[[Category: Mycobacterium tuberculosis]]
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<div style="clear:both"></div>
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[[Category: Single protein]]
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__TOC__
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[[Category: Alzari, P M.]]
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</StructureSection>
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[[Category: Betton, J M.]]
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[[Category: Large Structures]]
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[[Category: Buschiazzo, A.]]
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[[Category: Mycobacterium tuberculosis H37Rv]]
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[[Category: Cole, S T.]]
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[[Category: Alzari PM]]
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[[Category: Grana, M.]]
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[[Category: Betton JM]]
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[[Category: Haouz, A.]]
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[[Category: Buschiazzo A]]
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[[Category: SPINE, Structural Proteomics in Europe.]]
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[[Category: Cole ST]]
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[[Category: Shepard, W.]]
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[[Category: Grana M]]
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[[Category: DIO]]
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[[Category: Haouz A]]
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[[Category: GOL]]
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[[Category: Shepard W]]
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[[Category: conserved hypothetical]]
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[[Category: fatty acid binding protein like]]
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[[Category: restricted to actinomycete]]
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[[Category: spine]]
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[[Category: structural genomic]]
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[[Category: structural proteomics in europe]]
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[[Category: unknown function]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 16:57:43 2008''
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Current revision

Crystal Structure of Rv0813

PDB ID 2fwv

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