2fyh
From Proteopedia
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| - | [[Image:2fyh.jpg|left|200px]] | ||
| - | + | ==Solution structure of the 2'-5' RNA ligase-like protein from Pyrococcus furiosus== | |
| - | + | <StructureSection load='2fyh' size='340' side='right'caption='[[2fyh]]' scene=''> | |
| - | + | == Structural highlights == | |
| - | + | <table><tr><td colspan='2'>[[2fyh]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Pyrococcus_furiosus_DSM_3638 Pyrococcus furiosus DSM 3638]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2FYH OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2FYH FirstGlance]. <br> | |
| - | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr> | |
| - | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2fyh FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2fyh OCA], [https://pdbe.org/2fyh PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2fyh RCSB], [https://www.ebi.ac.uk/pdbsum/2fyh PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2fyh ProSAT], [https://www.topsan.org/Proteins/RSGI/2fyh TOPSAN]</span></td></tr> | |
| - | + | </table> | |
| - | + | == Function == | |
| - | + | [https://www.uniprot.org/uniprot/THPR_PYRFU THPR_PYRFU] Hydrolyzes RNA 2',3'-cyclic phosphodiester to an RNA 2'-phosphomonoester. In vitro, ligates 5' and 3' half-tRNA molecules with 2',3'-cyclic phosphate and 5'-hydroxyl termini, respectively, to the product containing the 2'-5' phosphodiester linkage. Ligase activity requires GTP, but GTP hydrolysis is not required for the reaction, which is reversible. Ligase activity is weak compared to the phosphodiesterase activity.<ref>PMID:19155324</ref> | |
| - | + | == Evolutionary Conservation == | |
| - | + | [[Image:Consurf_key_small.gif|200px|right]] | |
| - | == | + | Check<jmol> |
| - | + | <jmolCheckbox> | |
| - | [ | + | <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/fy/2fyh_consurf.spt"</scriptWhenChecked> |
| - | [[ | + | <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked> |
| - | [ | + | <text>to colour the structure by Evolutionary Conservation</text> |
| - | [[ | + | </jmolCheckbox> |
| - | [ | + | </jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2fyh ConSurf]. |
| - | [ | + | <div style="clear:both"></div> |
| - | [[ | + | == References == |
| - | [ | + | <references/> |
| - | [[ | + | __TOC__ |
| - | + | </StructureSection> | |
| - | [[Category: | + | [[Category: Large Structures]] |
| - | [[Category: | + | [[Category: Pyrococcus furiosus DSM 3638]] |
| - | [[Category: | + | [[Category: Kanai A]] |
| - | [[Category: | + | [[Category: Kawai G]] |
| - | [[Category: | + | [[Category: Matsuda T]] |
| - | [[Category: | + | [[Category: Muto Y]] |
| - | [[Category: | + | [[Category: Okada K]] |
| - | [[Category: | + | [[Category: Sakamoto T]] |
| - | [[Category: | + | [[Category: Yokoyama S]] |
| - | + | ||
| - | + | ||
Current revision
Solution structure of the 2'-5' RNA ligase-like protein from Pyrococcus furiosus
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