2fz4

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
Current revision (09:25, 14 February 2024) (edit) (undo)
 
(12 intermediate revisions not shown.)
Line 1: Line 1:
-
[[Image:2fz4.gif|left|200px]]
 
-
{{Structure
+
==Crystal Structure of the N-terminal half of Archaeoglobus Fulgidus XPB==
-
|PDB= 2fz4 |SIZE=350|CAPTION= <scene name='initialview01'>2fz4</scene>, resolution 2.40&Aring;
+
<StructureSection load='2fz4' size='340' side='right'caption='[[2fz4]], [[Resolution|resolution]] 2.40&Aring;' scene=''>
-
|SITE=
+
== Structural highlights ==
-
|LIGAND=
+
<table><tr><td colspan='2'>[[2fz4]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Archaeoglobus_fulgidus_DSM_4304 Archaeoglobus fulgidus DSM 4304]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2FZ4 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2FZ4 FirstGlance]. <br>
-
|ACTIVITY=
+
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.4&#8491;</td></tr>
-
|GENE= RAD25 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=224325 Archaeoglobus fulgidus DSM 4304])
+
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2fz4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2fz4 OCA], [https://pdbe.org/2fz4 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2fz4 RCSB], [https://www.ebi.ac.uk/pdbsum/2fz4 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2fz4 ProSAT]</span></td></tr>
-
}}
+
</table>
-
 
+
== Function ==
-
'''Crystal Structure of the N-terminal half of Archaeoglobus Fulgidus XPB'''
+
[https://www.uniprot.org/uniprot/O29889_ARCFU O29889_ARCFU]
-
 
+
== Evolutionary Conservation ==
-
 
+
[[Image:Consurf_key_small.gif|200px|right]]
-
==Overview==
+
Check<jmol>
-
The human xeroderma pigmentosum group B (XPB) helicase is essential for transcription, nucleotide excision repair, and TFIIH functional assembly. Here, we determined crystal structures of an Archaeoglobus fulgidus XPB homolog (AfXPB) that characterize two RecA-like XPB helicase domains and discover a DNA damage recognition domain (DRD), a unique RED motif, a flexible thumb motif (ThM), and implied conformational changes within a conserved functional core. RED motif mutations dramatically reduce helicase activity, and the DRD and ThM, which flank the RED motif, appear structurally as well as functionally analogous to the MutS mismatch recognition and DNA polymerase thumb domains. Substrate specificity is altered by DNA damage, such that AfXPB unwinds dsDNA with 3' extensions, but not blunt-ended dsDNA, unless it contains a lesion, as shown for CPD or (6-4) photoproducts. Together, these results provide an unexpected mechanism of DNA unwinding with implications for XPB damage verification in nucleotide excision repair.
+
<jmolCheckbox>
-
 
+
<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/fz/2fz4_consurf.spt"</scriptWhenChecked>
-
==About this Structure==
+
<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
-
2FZ4 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Archaeoglobus_fulgidus_dsm_4304 Archaeoglobus fulgidus dsm 4304]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2FZ4 OCA].
+
<text>to colour the structure by Evolutionary Conservation</text>
-
 
+
</jmolCheckbox>
-
==Reference==
+
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2fz4 ConSurf].
-
Conserved XPB core structure and motifs for DNA unwinding: implications for pathway selection of transcription or excision repair., Fan L, Arvai AS, Cooper PK, Iwai S, Hanaoka F, Tainer JA, Mol Cell. 2006 Apr 7;22(1):27-37. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/16600867 16600867]
+
<div style="clear:both"></div>
-
[[Category: Archaeoglobus fulgidus dsm 4304]]
+
__TOC__
-
[[Category: Single protein]]
+
</StructureSection>
-
[[Category: Fan, L.]]
+
[[Category: Archaeoglobus fulgidus DSM 4304]]
-
[[Category: Tainer, J A.]]
+
[[Category: Large Structures]]
-
[[Category: dna damage recognition domain]]
+
[[Category: Fan L]]
-
[[Category: reca-like domain]]
+
[[Category: Tainer JA]]
-
 
+
-
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 16:58:32 2008''
+

Current revision

Crystal Structure of the N-terminal half of Archaeoglobus Fulgidus XPB

PDB ID 2fz4

Drag the structure with the mouse to rotate

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/2fz4"
Personal tools