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| - | [[Image:2g5q.gif|left|200px]] | + | #REDIRECT [[4p20]] This PDB entry is obsolete and replaced by 4p20 |
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| - | {{Structure
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| - | |PDB= 2g5q |SIZE=350|CAPTION= <scene name='initialview01'>2g5q</scene>, resolution 2.700Å
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| - | |SITE=
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| - | |LIGAND= <scene name='pdbligand=AKN:(2R)-4-AMINO-N-{(1R,2S,3S,4R,5S)-5-AMINO-2-[(3-AMINO-3-DEOXY-ALPHA-D-GLUCOPYRANOSYL)OXY]-4-[(6-AMINO-6-DEOXY-ALPHA-D-GLUCOPYRANOSYL)OXY]-3-HYDROXYCYCLOHEXYL}-2-HYDROXYBUTANAMIDE'>AKN</scene>
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| - | |ACTIVITY=
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| - | |GENE=
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| - | }}
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| - | '''Crystal structures of the bacterial ribosomal decoding site complexed with amikacin.'''
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| - | ==Overview==
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| - | Amikacin is the 4,6-linked aminoglycoside modified at position N1 of the 2-deoxystreptamine ring (ring II) by the L-haba group. In the present study, the crystal structure of a complex between oligonucleotide containing the bacterial ribosomal A site and amikacin has been solved at 2.7 A resolution. Amikacin specifically binds to the A site in practically the same way as its parent compound kanamycin. In addition, the L-haba group interacts with the upper side of the A site through two direct contacts, O2*...H-N4(C1496) and N4*-H...O6(G1497). The present crystal structure shows how the introduction of the L-haba group on ring II of aminoglycoside is an effective mutation for obtaining a higher affinity to the bacterial A site.
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| - | ==About this Structure==
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| - | 2G5Q is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/ ]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2G5Q OCA].
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| - | ==Reference==
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| - | Crystal structure of the bacterial ribosomal decoding site complexed with amikacin containing the gamma-amino-alpha-hydroxybutyryl (haba) group., Kondo J, Francois B, Russell RJ, Murray JB, Westhof E, Biochimie. 2006 Aug;88(8):1027-31. Epub 2006 Jun 13. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/16806634 16806634]
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| - | [[Category: Protein complex]]
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| - | [[Category: Francois, B.]]
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| - | [[Category: Kondo, J.]]
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| - | [[Category: Murray, J B.]]
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| - | [[Category: Russell, R J.M.]]
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| - | [[Category: Westhof, E.]]
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| - | [[Category: AKN]]
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| - | [[Category: amikacin]]
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| - | [[Category: aminoglycoside]]
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| - | [[Category: haba group]]
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| - | [[Category: ribosomal decoding site]]
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| - | [[Category: rna]]
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| - | [[Category: x-ray analysis]]
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| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 17:00:44 2008''
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